Süt Tozu-Yumurta Sarısı Sulandırıcısına Balık Yağı İlavesinin Dondurulmuş-Çözdürülmüş Bali Boğa Spermatozoonlarının Kalitesi Üzerine Etkisi

Malik, Abdul; Syarifdjaya, Muhammad; Gunawan, Aam; Erlina, Siti; Jaelani, Achmad; Wibowo, Dadang Budi

doi:10.9775/kvfd.2017.17556

Cited by 2 publications

(3 citation statements)

References 20 publications

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“…The microscopic analysis included analyses of sperm concentration, viability, motility and plasma membrane integrity. The basic extender used in this research contained skim milk, glucose, egg yolks, glucose, penicillin or streptomycin, and glycerol adopted from Malik et al (2017). Honey was added inextender with concentrations of 0% (control), 2%, 4%, 6% and 8% modification with .…”

Section: Animalsmentioning

confidence: 99%

“…The volume of semen was measured inmicroliters using a micropipette. The motility of the sperm was evaluated by placing a drop of semen sample on a pre-warmed (37°C) glass slide under a light microscope (400×) using the methodology of Malik et al (2017). The percentage of sperm motility was subjectively assessed on a scale ranging from 0 to 100%.…”

Section: Semen Qualitymentioning

confidence: 99%

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Cryopreservative Effect of Adding a Honey Solution to Native Chicken Spermatozoa

Malik¹,

Indah²,

Z̲ākir³

et al. 2019

Adv. Anim. Vet. Sci.

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The current study was conducted to determine the quality of semen a native chicken Kalimantan breed. A total of four cocks were used in this study. Semen was collected twice per week via the dorsal-abdominal massage method. Semen qualities were very important to the success of fertility in the chicken. The semen was then assessed macroscopic and microscopic to determine viability, motility, integrity of the membrane plasma and to look for abnormalities. There were no significant differences (P>0.05) in sperm viability, abnormality or integrity of the membrane plasma between treatments and the control before freezing. However, sperm motility before freezing was significantly different (P<0.05) between the added 4% of honey (P4 treatment) semen and the controls. Semen qualities post-thawing such as viability, motility, abnormalities and integrity of the membrane plasma were not significantly different (P>0.05) between the controls and all treatments. The motility of spermatozoa before freezing for the P4 treatment was significantly lower than that control and all treatments, and the addition of honey with 2, 4, 6, 8% has no effect on semen qualities in post-thawed chicken spermatozoa.

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Section: Animalsmentioning

confidence: 99%

Section: Semen Qualitymentioning

confidence: 99%

Cryopreservative Effect of Adding a Honey Solution to Native Chicken Spermatozoa

Malik¹,

Indah²,

Z̲ākir³

et al. 2019

Adv. Anim. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…Artificial insemination is one method that could be utilized to increase the population of cattle and buffalo. Artificial insemination (AI) serves as an essential and effective tool for genetic enhancement in various livestock species, including buffalo, horses, cattle, goats, sheep, and poultry (Malik et al, 2017). One crucial factor influencing the success of artificial insemination (AI) is the quality of post-thawed spermatozoa, which is controlled by the composition of the cryopreservation medium.…”

Section: Introductionmentioning

confidence: 99%

Frozen Semen Quality of Kalang Buffalo Using Lycopene

Elvania,

Rostini,

Sakiman

et al. 2024

AAVS

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An important factor to successful of artificial insemination (AI) in buffalo was the quality of spermatozoa post-thawed. Therefore, research was conducted on the use of lycopene as an effort to improve the quality of spermatozoa post-thawing. This study aimed to assess the impact of incorporating lycopene into both the skim milk and egg yolk extender at varying doses on pre-freeze and post-thawed Kalang buffalo semen. Four Kalang buffalo, aged between 6 to 7 years and weighing 500-550 kg, were involved in this investigation. Semen collection was conducted weekly a 12-week period using an artificial vagina. Lycopene was added to the skim milk-egg yolk diluent at concentrations of 1%, 2%, 3%, and 4%, while the control group received no lycopene substitution. The freshly collected semen underwent macroscopic and microscopic evaluations. Subsequently, the semen was assessed pre-freezing and post-thaw with parameters such as viability, motility, abnormality, and plasma membrane integrity. The findings revealed that the inclusion of 1% and 2% lycopene in the diluent before freezing exhibited significantly higher (P < 0.05) spermatozoa motility. Moreover, the viability and motility of post-thawed semen significantly improved (P < 0.05) at lycopene doses of 1% and 2%. The addition of 1% to 2% lycopene in the extender demonstrated a positive effect on the sperm qualities, particularly enhancing the viability and motility of post-thaw semen from Kalang buffalo.

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Süt Tozu-Yumurta Sarısı Sulandırıcısına Balık Yağı İlavesinin Dondurulmuş-Çözdürülmüş Bali Boğa Spermatozoonlarının Kalitesi Üzerine Etkisi

Cited by 2 publications

References 20 publications

Cryopreservative Effect of Adding a Honey Solution to Native Chicken Spermatozoa

Cryopreservative Effect of Adding a Honey Solution to Native Chicken Spermatozoa

Frozen Semen Quality of Kalang Buffalo Using Lycopene

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