Sustainable release of vancomycin, gentamicin and lidocaine from novel electrospun sandwich-structured PLGA/collagen nanofibrous membranes

Chen, Dave W.; Hsu, Yung‐Heng; Liao, Junyi; Liu, Shih‐Jung; Chen, Jan-Kan; Ueng, Steve Wen–Neng

doi:10.1016/j.ijpharm.2012.04.010

Cited by 108 publications

(49 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among various polymeric materials available for a local release system, PLGA is a promising biodegradable material that allows sustained delivery of high antibiotic concentrations at the target site. [9][10][11][12][13] The copolymer has also been approved for clinical applications owing to its nontoxicity, negligible inflammatory reaction, and biocompatible hydrolysis products (lactic and glycolic acids) after biodegradation. 10,14,15 To prepare biodegradable sheath-core nanofibers, predetermined weight percentages of PLGA, vancomycin, and ceftazidime were dissolved in hexafluoroisopropyl alcohol (HFIP), and rhBMP-2 was dissolved in phosphate-buffered solution.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers

Hsu

Lin²,

et al. 2016

IJN

Self Cite

View full text Add to dashboard Cite

In this study, we developed biodegradable sheath-core-structured drug-eluting nanofibers for sustainable delivery of antibiotics (vancomycin and ceftazidime) and recombinant human bone morphogenetic protein (rhBMP-2) via electrospinning. To prepare the biodegradable sheath-core nanofibers, we first prepared solutions of poly( d , l )-lactide- co -glycolide, vancomycin, and ceftazidime in 1,1,1,3,3,3-hexafluoro-2-propanol and rhBMP-2 in phosphate-buffered solution. The poly( d , l )-lactide- co -glycolide/antibiotics and rhBMP-2 solutions were then fed into two different capillary tubes controlled by two independent pumps for coaxial electrospinning. The electrospun nanofiber morphology was observed under a scanning electron microscope. We further characterized the in vitro antibiotic release from the nanofibers via high-performance liquid chromatography and that of rhBMP-2 via enzyme-linked immunosorbent assay and alkaline phosphatase activity. We showed that the biodegradable coaxially electrospun nanofibers could release high vancomycin/ceftazidime concentrations (well above the minimum inhibition concentration [MIC] 90 ) and rhBMP-2 for >4 weeks. These experimental results demonstrate that novel biodegradable nanofibers can be constructed with various pharmaceuticals and proteins for long-term drug deliveries.

show abstract

Section: Introductionmentioning

confidence: 99%

“…After the electrospinning process, the experimental results also showed high level of bioactivities for the released vancomycin and ceftazidime. 8,10 Furthermore, the experimental results demonstrated that the fabrication process for the biodegradable sheath-corestructured nanofiber membranes was appropriate.…”

mentioning

confidence: 95%

Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers

Hsu

Lin²,

et al. 2016

IJN

Self Cite

View full text Add to dashboard Cite

show abstract

“…Natural polymers are more capable of mimicking an extracellular matrix, whereas the synthetic polymers loaded with drugs can be easily electrospun ( Figure 6) [72]. Chen et al [73] synthesized sandwiched drug eluting membranes made up of an outer layer of PLGA/collagen and core layers of PLGA/Vancomycin, gentamicin and lidocaine. The drug release for vancomycin and gentamicin was found to last four and three weeks respectively.…”

Section: Polymer-based Nanofibers For Drug Deliverymentioning

confidence: 99%

Multifunctional Nanofibers towards Active Biomedical Therapeutics

et al. 2015

View full text Add to dashboard Cite

One-dimensional (1-D) nanostructures have attracted enormous research interest due to their unique physicochemical properties and wide application potential. These 1-D nanofibers are being increasingly applied to biomedical fields owing to their high surface area-to-volume ratio, high porosity, and the ease of tuning their structures, functionalities, and properties. Many biomedical nanofiber reviews have focused on tissue engineering and drug delivery applications but have very rarely discussed their use as wound dressings. However, nanofibers have enormous potential as wound dressings and other clinical applications that could have wide impacts on the treatment of wounds. Herein, the authors review the main fabrication methods of nanofibers as well as requirements, strategies, and recent applications of nanofibers, and provide perspectives of the challenges and opportunities that face multifunctional nanofibers for active therapeutic applications.

show abstract

“…[6][7][8] PLGA is a promising biodegradable material that has been used for implantable and injectable controlled-release drug-delivery systems. 9,10 The copolymer has also been approved for clinical applications because it is nontoxic, instigates a minimal inflammatory response, and is biodegradable through the hydrolysis of its ester linkage to transform biocompatible lactic and glycolic acids.…”

Section: Introductionmentioning

confidence: 99%

“…Various types of carrier materials have been used based on their ability to achieve sustained release of bactericidal concentrations of antibiotics. [6][7][8] Nonbiodegradable PMMA antibiotic-impregnated beads are most commonly used in the treatment of osteomyelitis. 4,17 However, when the drug is completely released, the PMMA beads act as a biomaterial surface to which bacteria adhere and grow, which might potentially cause the development of antibiotic resistance.…”

mentioning

confidence: 99%

Biodegradable drug-eluting nanofiber-enveloped implants for sustained release of high bactericidal concentrations of vancomycin and ceftazidime: in vitro and in vivo studies

Hsu¹,

Chen²,

Tai³

et al. 2014

IJN

Self Cite

View full text Add to dashboard Cite

We developed biodegradable drug-eluting nanofiber-enveloped implants that provided sustained release of vancomycin and ceftazidime. To prepare the biodegradable nanofibrous membranes, poly(D,L)-lactide-co-glycolide and the antibiotics were first dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol. They were electrospun into biodegradable drug-eluting membranes, which were then enveloped on the surface of stainless plates. An elution method and a high-performance liquid chromatography assay were employed to characterize the in vivo and in vitro release rates of the antibiotics from the nanofiber-enveloped plates. The results showed that the biodegradable nanofiber-enveloped plates released high concentrations of vancomycin and ceftazidime (well above the minimum inhibitory concentration) for more than 3 and 8 weeks in vitro and in vivo, respectively. A bacterial inhibition test was carried out to determine the relative activity of the released antibiotics. The bioactivity ranged from 25% to 100%. In addition, the serum creatinine level remained within the normal range, suggesting that the high vancomycin concentration did not affect renal function. By adopting the electrospinning technique, we will be able to manufacture biodegradable drug-eluting implants for the long-term drug delivery of different antibiotics.

show abstract

Sustainable release of vancomycin, gentamicin and lidocaine from novel electrospun sandwich-structured PLGA/collagen nanofibrous membranes

Cited by 108 publications

References 26 publications

Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers

Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers

Multifunctional Nanofibers towards Active Biomedical Therapeutics

Biodegradable drug-eluting nanofiber-enveloped implants for sustained release of high bactericidal concentrations of vancomycin and ceftazidime: in vitro and in vivo studies

Contact Info

Product

Resources

About