Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers

Abstract

In this study, we developed biodegradable sheath-core-structured drug-eluting nanofibers for sustainable delivery of antibiotics (vancomycin and ceftazidime) and recombinant human bone morphogenetic protein (rhBMP-2) via electrospinning. To prepare the biodegradable sheath-core nanofibers, we first prepared solutions of poly( d , l )-lactide- co -glycolide, vancomycin, and ceftazidime in 1,1,1,3,3,3-hexafluoro-2-propanol and rhBMP-2 in phosphate-buffered solution. The poly( d , l )-lactide- co -glycolide/antibiotics and rhBMP-2 solutions were then fed into two different capillary tubes controlled by two independent pumps for coaxial electrospinning. The electrospun nanofiber morphology was observed under a scanning electron microscope. We further characterized the in vitro antibiotic release from the nanofibers via high-performance liquid chromatography and that of rhBMP-2 via enzyme-linked immunosorbent assay and alkaline phosphatase activity. We showed that the biodegradable coaxially electrospun nanofibers could release high vancomycin/ceftazidime concentrations (well above the minimum inhibition concentration [MIC] 90 ) and rhBMP-2 for >4 weeks. These experimental results demonstrate that novel biodegradable nanofibers can be constructed with various pharmaceuticals and proteins for long-term drug deliveries.

Synthesis of Chitosan-Based Hydrogels as a Novel Drug Release Device for Wound Healing

“…PVA-CS-PCL hydrogels bear high content of hydroxyl groups that are accessible to form hydrogen bonds with ceftazidime and ketoprofen molecules. The results confirmed the successful incorporation of the drug molecules in the hydrogel structure [25].…”

Fabrication of Antibacterial Nanofibrous Membrane Infused with Essential Oil Extracted from Tea Tree for Packaging Applications

“…The histogram of fiber diameter was drawn by measuring more than 50 nanofibers randomly from the SEM images using Image J program. The mechanical properties of the nanofiber were obtained using a universal test machine (UT-020E, MTDI INC, Daejeon, Korea) using a dog bone specimen according to the ASTM D638 protocol [21][22][23][24]. The functional groups and crystalline of surface chemistry were examined by Fourier transform infrared spectrometer (Frontier, Perkin Elmer Co., Waltham, The Commonwealth of Massachusetts, USA) and multi-purpose high performance X-ray diffractometer (X'pert Pro Powder, PANalyrical, Almelo, The Netherlands) to compare PU nanofibers with PU-5% TT oil sample.…”

Protein delivery nanosystem of six-arm copolymer poly(ε-caprolactone)–poly(ethylene glycol) for long-term sustained release

“…25,27,49,50 The time of sustainable protein release in this study was approximately 1 month, which was longer than in other reports. 8,51,52 The reason for this could be that PVA increases the viscosity of the external water phase and results in increased difficulty for the OVA aqueous solution to diffuse out. 53 Overall, the NPs were effective protein delivery carriers, with suitable size, loading capacity, stability, and sustained-release properties.…”