Sustainable Agriculture and Food Security

Umesha, S.; Manukumar, H.M.; Chandrasekhar, B.

doi:10.1016/b978-0-12-812160-3.00003-9

Cited by 54 publications

(42 citation statements)

References 16 publications

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“…In summary, the genomic DNA products obtained with our genomic DNA extraction method are completely suitable for PCR-based applications. The method described in this study is significantly uncomplicated in terms of execution and considerably more secure, since it does not employ toxic chemicals such as phenol and chloroform like other DNA extraction methods [17,[28][29][30]. Our method further provides higher DNA concentrations as compared to the simple method reported by Cenis (1992) [10], and even the DNA concentrations are 10 times higher in comparison to genomic DNA concentrations obtained from the phenol-chloroform method employed by Umesha (2016) [30].…”

Section: Fig 3 Extraction Of Genomic Dna From Different Molds (A)mentioning

confidence: 81%

A simple, efficient and universal method for the extraction of genomic DNA from bacteria, yeasts, molds and microalgae suitable for PCR-based applications

Loc¹,

Tran²,

Nguyen³

et al. 2017

VJSTE

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The extraction of genomic DNA from microbial cells plays a significant role in PCR-based applications such as molecular diagnosis, microbial taxonomy, screening of genetically engineered microorganisms, and other such PCRbased applications. Currently, many methods for extraction of genomic DNA from microorganisms have been developed. However, these methods either require hazardous chemicals or consist of time-consuming steps for effective execution. In this study, we have established a simple and universal genomic DNA extraction method for different microorganisms including bacteria, yeasts, molds, and microalgae. Our method does not require harmful reagents such as phenol and chloroform for the extraction process to minimize the generation of hazardous wastes. The obtained genomic DNA products displayed high concentrations and represented a good purity level with the average 260 nm/280 nm absorbance ratios (A 260/280) that range from 1.6 to 2.0. The DNA molecules further remained considerably intact when analyzed on agarose gels. More importantly, these DNA products were qualified through successful PCR amplifications of 16S rRNA gene, rDNA internal transcribed spacer (ITS), or 18S rRNA gene from genomes of bacteria, fungi, and microalgae respectively. Furthermore, with the extracted genomic DNA products, the processes of the identification of the haploid and diploid states of the Saccharomyces yeast strains or detection of putative strains of Aspergillus oryzae and Aspergillus flavus that have been isolated from infected food materials through PCR analyses are facilitated. The genomic DNA extraction method established in this study is easy to manage, time saving and costeffective, and environmentally friendly.

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Section: Fig 3 Extraction Of Genomic Dna From Different Molds (A)mentioning

confidence: 81%

A simple, efficient and universal method for the extraction of genomic DNA from bacteria, yeasts, molds and microalgae suitable for PCR-based applications

Loc¹,

Tran²,

Nguyen³

et al. 2017

VJSTE

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“…a nasal swab), real time or quantitative PCR (qPCR) has been able to address some of the issues associated with culture-based methods (although some PCRbased methodologies require broth enrichment). Key developments in qPCR have resulted in signicant diagnostic performance advantages over conventional PCR; for example, qPCR methods require small quantities of sample and provide rapid results, 43,44 and also require minimal sample manipulation (thus further reducing the time required and the risk of contamination) due to the measurement of changes in the uorescence signal from the start of the PCR process.…”

Section: Nucleic Acidsmentioning

confidence: 99%

Novel diagnostics for point-of-care bacterial detection and identification

et al. 2019

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“…The consumption of regional fruit, taking advantage of all its parts, can be a strategy to improve the state of nutrition of families and promote a sustainable agriculture, valuing the local climate, because it favors less intervention for fruit production, and also valuing the regional culture of food (Costa et al, 2016;Umesha et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of cookies using passion fruit from the caatinga (Passiflora cincinnata Mast.)

et al. 2022

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The objective of this study was to use the passion fruit peel P. cincinnata Mast., as a viable alternative to produce food (flour and cookies) that is rich in fiber and environmentally sustainable. For this, fruit peels were obtained, which were hygienized, dehydrated and ground in a knife mill in order to develop the flour, which was then used in different concentrations in three formulations of chip cookies (using 10%, 20% and 30% of the flour developed). Analyzes physicochemical and microbiological composition were performed for both flour and for the cookies developed and a sensorial acceptance test was performed for the cookies. It was possible to observe that the flour had an adequate centesimal composition, when it was about quality and safety, as well as health benefits. As for the physicochemical composition, the flour had 5.79 ± 0.11% of ashes; 78.23 ± 0.84% of total carbohydrates; 5.78 ± 0.11% moisture; 4.83 ± 0.00% acidity; 3.74 ± 0.04 pH; 1.66 ± 0.06% lipids; 8.59 ± 0.82% protein and 58,3 g/100g dietary fiber. The study demonstrates its nutritional quality by reducing the lipid content of the final product, by comparing the formulation with 30% of the flour developed to the standard formulation, as well as increasing the ash content and acidity and reducing its pH when compared to the standard formulation, promoting the safety and quality of food. Flour presented promising capacity in the development of functional foods, health promoters, and environmental conservation by avoiding its inappropriate disposal.

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Sustainable Agriculture and Food Security

Cited by 54 publications

References 16 publications

A simple, efficient and universal method for the extraction of genomic DNA from bacteria, yeasts, molds and microalgae suitable for PCR-based applications

A simple, efficient and universal method for the extraction of genomic DNA from bacteria, yeasts, molds and microalgae suitable for PCR-based applications

Novel diagnostics for point-of-care bacterial detection and identification

Development and characterization of cookies using passion fruit from the caatinga (Passiflora cincinnata Mast.)

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