Susceptibility to insecticides and resistance mechanisms in Aedes aegypti from the Colombian Caribbean Region

Maestre‐Serrano, Ronald; Gómez‐Camargo, Doris; Ponce‐García, Gustavo; Flores, Adriana E.

doi:10.1016/j.pestbp.2014.09.014

Cited by 48 publications

(127 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…En lo que respecta a Colombia, en poblaciones de A. aegypti de los municipios de Puerto Colombia, Soledad, Valledupar, San Juan del Cesar, Sincelejo, Montería y Ciénaga, y los distritos de Barranquilla y Cartagena en la región Caribe, se encontró la mutación kdr Val1016Ile, la cual se ha relacionado con resistencia a los piretroides deltametrina, ciflutrín y permetrina, pero no a lambdacialotrina (15), en mosquitos que portaban la mutación en condición homocigota.…”

Section: Discussionunclassified

“…El efecto fenotípico de estas mutaciones es la resistencia al "derribo" o kdr (knock-down resistance), mecanismo que confiere resistencia cruzada entre los insecticidas piretroides y el DDT (12 (14). En el 2014, Maestre, et al (15), reportaron en la población de A. aegypti de Sincelejo poca resistencia a la permetrina y el ciflutrín, resistencia moderada a la deltametrina y el metil-pirimifós y alta resistencia a la lambdacialotrina y el DDT. En este estudio también se confirmó la sensibilidad al malatión y el fenitrotión.…”

unclassified

See 1 more Smart Citation

Primer reporte de la mutación F1534C, asociada con resistencia cruzada a DDT y piretroides, en Aedes aegypti de Colombia

et al. 2016

View full text Add to dashboard Cite

Introducción. La principal estrategia para el control de Aedes aegypti, vector de los virus dengue, chikungunya y Zika, se basa en la utilización de insecticidas con el fin de disminuir las poblaciones del vector. Sin embargo, su uso ha conllevado a que el insecto desarrolle resistencia a estos químicos.Objetivo. Determinar la presencia de la mutación F1534C, asociada con resistencia cruzada a DDT y piretroides, en mosquitos de la especie Ae. aegypti de la ciudad de Sincelejo, Colombia.Materiales y métodos. El estudio se desarrolló con nueve ejemplares de Ae. aegypti que mostraron resistencia a lambdacialotrina en bioensayos desarrollados por la Secretaría de Salud de Sucre. Se realizó una PCR semi-anidada, según la la metodología descrita por Harris y colaboradores (2010), con el fin de amplificar el exón 31 del gen para del canal de sodio dependiente de voltaje de Ae. aegypti. Los productos de PCR se secuenciaron, editaron y analizaron con el programa MEGA 5.Resultados. En todos los mosquitos evaluados se detectó la presencia del alelo silvestre y mutante del exón 31. En la secuencia nucleotídica del alelo mutante, se observó una sustitución de timina por guanina, que produce el cambio del codón UUC por UGC y conlleva al reemplazo del aminoácido fenilalanina por cisteína en el residuo 1534 de la proteína.Conclusión. Los nueve mosquitos analizados presentaron un genotipo heterocigoto para la mutación F1534C, cuyo efecto fenotípico es la resistencia al derribo (kdr) por los insecticidas DDT y piretroides.

show abstract

Section: Discussionunclassified

unclassified

Primer reporte de la mutación F1534C, asociada con resistencia cruzada a DDT y piretroides, en Aedes aegypti de Colombia

et al. 2016

View full text Add to dashboard Cite

show abstract

“…We included nine populations of Aedes aegypti collected between 2010 and 2011 in the Colombian Caribbean region and whose levels of resistance to permethrin, deltamethrin, λ‐cyhalothrin and cyfluthrin were previously reported and are also included in Table . The locations of the sampling sites were Barranquilla (10° 57′ 50″ N, 74° 47′ 47″ W), Puerto Colombia (10° 59′ 16″ N, 74° 57′ 17″ W) and Soledad (10° 55′ 06″ N, 74° 45′ 53″ W) in the state of Atlantico; Valledupar (10° 27′ 47″ N, 73° 15′ 12″ W) in the state of Cesar; San Juan del Cesar (10° 46′ 16″ N, 73° 00′ 11″ W) in the state of La Guajira; Sincelejo (09° 18′ 17″ N, 75° 23′ 52″ W) in the state of Sucre; Monteria (08° 47′ 05″ N, 75° 52′ 53″ W) in the state of Cordoba; Cienaga (11° 00′ 25″ N, 74° 14′ 52″ W) in the state of Magdalena; and Cartagena (10° 23′ 59″ N, 75° 30′ 52″ W) in the state of Bolivar (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Aedes aegypti is the main vector of dengue, chikungunya and Zika viruses, of public health importance in Colombia. For the control of A. aegypti and in the absence of an effective vaccine for the prevention of diseases it causes, insecticides including organochlorines, organophosphates, carbamates and pyrethroids have been used; however, the continuous use of these chemicals has generated resistance in populations of this species in Colombia, mainly to DDT and pyrethroids . Knockdown resistance to these insecticides is associated with the presence of non‐synonymous mutations that reduce pyrethroids binding to voltage‐dependent sodium channels .…”

Section: Introductionmentioning

confidence: 99%

Co‐occurrence of V1016I and F1534C mutations in the voltage‐gated sodium channel and resistance to pyrethroids in Aedes aegypti (L.) from the Colombian Caribbean region

Maestre‐Serrano

Pareja-Loaiza

Camargo

et al. 2019

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND Knockdown resistance is conferred primarily by non‐synonymous mutations that reduce pyrethroids binding to voltage‐gated sodium channels. In 2014, kdr mutation V1016I in Aedes aegypti populations resistant to pyrethroids was reported for the first time in Colombiα, in 2016 another kdr mutation, F1534C, and in 2018 the mutation V419L. Nine populations of A. aegypti, previously characterized as being resistant to λ‐cyhalothrin, deltamethrin, cyfluthrin and permethrin, were used for this study. Genomic DNA was used to determine genotypes by allele‐specific PCR for mutations V1016I and F1534C, and to determine their association with pyrethroid resistance. RESULTS All the populations analyzed showed both mutations, with allelic frequencies of 0.07–0.35 for I1016 and 0.47–0.88 for C1534. A percentage of co‐occurrence of mutant homozygotes I1016/C1534 of 5.3% was detected. A significant positive correlation was found between the frequency of the genotype I1016 and the resistance to permethrin, λ‐cyhalothrin and cyfluthrin, but not to deltamethrin; on the other hand, the correlation was not significant for the C1534 genotype and the four pyrethroids evaluated. No significant correlation was found between the frequencies of the mutations V1016I and F1534C. CONCLUSIONS Both mutations V1016I and F1534C are present in A. aegypti populations of the Colombian Caribbean, and although the frequency of F1534C exceeds V1016I, the latter was correlated to resistance to pyrethroid insecticides. © 2018 Society of Chemical Industry

show abstract

“…Subsequent studies reported the presence of kdr conferring mutations in Ae. aegypti collections from Brazil (Martins et al, 2009a(Martins et al, , 2009bLima et al, 2011;Belinato, Martin and Valle, 2012), Mexico (Ponce-Garcia et al, 2009;Siller et al, 2011;Aponte et al, 2013) and the Caribbean (Marcombe et al, 2009(Marcombe et al, , 2012(Marcombe et al, , 2013Harris, Rajatileka and Ranson, 2010;Bariami et al, 2012;McAllister, Godsey and Scott, 2012;Maestre-Serrano et al, 2014;Alvarez et al, 2015).…”

Section: Target-site Resistancementioning

confidence: 99%

Abbreviations and acronyms

2018

Safety Assessment of Transgenic Organisms in the Environment, Volume 8

View full text Add to dashboard Cite

This work is published under the responsibility of the Secretary-General of the OECD. The opinions expressed and arguments employed herein do not necessarily reflect the official views of OECD member countries.This document, as well as any data and any map included herein, are without prejudice to the status of or sovereignty over any territory, to the delimitation of international frontiers and boundaries and to the name of any territory, city or area.Please cite this publication as: OECD (2018) The statistical data for Israel are supplied by and under the responsibility of the relevant Israeli authorities. The use of such data by the OECD is without prejudice to the status of the Golan Heights, East Jerusalem and Israeli settlements in the West Bank under the terms of international law.Photo credits: Cover © Maks Ershov.Corrigenda to OECD publications may be found on line at: www.oecd.org/about/publishing/corrigenda.htm. © OECD 2018You can copy, download or print OECD content for your own use, and you can include excerpts from OECD publications, databases and multimedia products in your own documents, presentations, blogs, websites and teaching materials, provided that suitable acknowledgment of the source and copyright owner(s) is given. All requests for public or commercial use and translation rights should be submitted to rights@oecd.org. Requests for permission to photocopy portions of this material for public or commercial use shall be addressed directly to the Copyright Clearance Center (CCC) at info@copyright.com or the Centre francais d'exploitation du droit de copie (CFC) at contact@cfcopies.com. FOREWORD │ 3SAFETY ASSESSMENTOF TRANSGENIC ORGANISMSIN THE ENVIRONMENT, VOLUME 8 © OECD 2018 ForewordModern biotechnologies are applied to plants species (crops, flowers, trees), animals and micro-organisms. The safety of the resulting transgenic organisms when released in the environment for their use in agriculture, forestry, the food and feed industry or for other applications represents a challenging issue. Genetically engineered products are rigorously assessed by their developers during their elaboration, and by governments when ready for release, to ensure high safety standards. This remains essential with new biotechnology developments using insects to fight against disease outbreaks: engineered mosquitoes need to be evaluated through a scientifically sound approach to risk/safety assessment that will inform biosafety regulators and support the decision concerning the release of these novel organisms in the environment.The OECD offers a long-standing recognised expertise in biosafety and contributes to facilitating an harmonised approach. The OECD's Working Group on Harmonisation of Regulatory Oversight in Biotechnology (WG-HROB) was established in 1995. The WG-HROB gathers national authorities responsible for the environmental risk/safety assessment of products of modern biotechnology in OECD countries and other economies. International organisations and experts involved in biosafety activities are a...

show abstract

Susceptibility to insecticides and resistance mechanisms in Aedes aegypti from the Colombian Caribbean Region

Cited by 48 publications

References 36 publications

Primer reporte de la mutación F1534C, asociada con resistencia cruzada a DDT y piretroides, en Aedes aegypti de Colombia

Primer reporte de la mutación F1534C, asociada con resistencia cruzada a DDT y piretroides, en Aedes aegypti de Colombia

Co‐occurrence of V1016I and F1534C mutations in the voltage‐gated sodium channel and resistance to pyrethroids in Aedes aegypti (L.) from the Colombian Caribbean region

Abbreviations and acronyms

Contact Info

Product

Resources

About