Primer reporte de la mutación F1534C, asociada con resistencia cruzada a DDT y piretroides, en Aedes aegypti de Colombia

Atencia, María Claudia; Pérez, María de Jesús; Jaramillo, María Cristina; Caldera, Sandy Milena; Cochero, Suljey; Bejarano, Eduar Elías

doi:10.7705/biomedica.v36i3.2834

Cited by 15 publications

(18 citation statements)

References 20 publications

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“…Our findings of resistance to λ-cyhalothrin in populations of Ae. aegypti in the Caribbean region of Colombia are consistent with those reported by Maestre et al [13] for the populations of Barranquilla and Montería, Granada et al [15] for the population of Riohacha, and Atencia et al [31] for the population of Sincelejo. Likewise, Maestre et al [13] reported resistance to λ-cyhalothrin in Valledupar and moderate resistance in Cartagena, while in the present work we detected resistance using the WHO bioassay but susceptibility using the CDC bioassay in both populations.…”

Section: Plos Onesupporting

confidence: 88%

“…The F1534C mutation was first detected in Colombia in the department of Sincelejo (Sucre), in the Caribbean region [31]. It had also previously been reported in Ae.…”

Section: Plos Onementioning

confidence: 82%

“…in the enzymatic activity of glutathione-S-transferases, alpha-esterases and mixed function oxidases [13]. Additionally, the kdr mutations V1016I [13,30], F1534C [31,32] and V410L [15] have been detected.…”

Section: Plos Onementioning

confidence: 99%

“…Moreover, increased activity levels of insecticide-degrading enzymes, such as nonspecific esterases, mixed-function oxidases (MFOs), glutathione S-transferases (GSTs), and insensitive acetylcholinesterase (iAChE), have been observed in resistant populations [9][10][11][12][13]24]. In addition, the kdr mutations V1016I [13,30], F1534C [31], and V410L [15] associated with pyrethroid resistance have recently been detected.…”

Section: Introductionmentioning

confidence: 99%

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Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia

et al. 2020

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Aedes aegypti is the main vector of dengue, chikungunya, and Zika viruses, which are of great public health importance in Colombia. Aedes control strategies in Colombia rely heavily on the use of organophosphate and pyrethroid insecticides, providing constant selection pressure and the emergence of resistant populations. In recent years, insecticide use has increased due to the increased incidence of dengue and recent introductions of chikungunya and Zika. In the present study, pyrethroid resistance was studied across six populations of Ae. aegypti from the Caribbean coast of Colombia. Susceptibility to λ-cyhalothrin, deltamethrin, and permethrin was assessed, and resistance intensity was determined. Activity levels of enzymes associated with resistance were measured, and the frequencies of three kdr alleles (V1016I, F1534C, V410L) were calculated. Results showed variations in pyrethroid susceptibility across Ae. aegypti populations and altered enzyme activity levels were detected. The kdr alleles were detected in all populations, with high variations in frequencies: V1016I (frequency ranging from 0.15-0.70), F1534C (range 0.94-1.00), and V410L (range 0.05-0.72). In assays of phenotyped individuals, associations were observed between the presence of V1016I, F1534C, and V410L alleles and resistance to the evaluated pyrethroids, as well as between the VI 1016 /CC 1534 /VL 410 tri-locus genotype and λ-cyhalothrin and permethrin resistance. The results of the present study contribute to the knowledge of the mechanisms underlying the resistance to key pyrethroids used to control Ae. aegypti along the Caribbean coast of Colombia.

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Section: Plos Onesupporting

confidence: 88%

“…The F1534C mutation was first detected in Colombia in the department of Sincelejo (Sucre), in the Caribbean region [31]. It had also previously been reported in Ae.…”

Section: Plos Onementioning

confidence: 82%

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia

et al. 2020

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“…aegypti populations from Latin America [ 9 ]. Also, the F1534C substitution in domain III-S6 was discovered in Vietnam and Thailand [ 10 , 11 , 12 ], and more recently in Brazil [ 13 ], Venezuela [ 14 ], and Colombia [ 15 ]. Finally, two additional mutations, S989P and D1763Y, were found in Asiatic countries [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

A Point Mutation V419L in the Sodium Channel Gene from Natural Populations of Aedes aegypti Is Involved in Resistance to λ-Cyhalothrin in Colombia

Granada

Mejía-Jaramillo

Strode

et al. 2018

Insects

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Resistance to pyrethroids in mosquitoes is mainly caused by target site insensitivity known as knockdown resistance (kdr). In this work, we examined the point mutations present in portions of domains I, II, III, and IV of the sodium channel gene in Aedes aegypti mosquitoes from three Colombian municipalities. A partial region coding for the sodium channel gene from resistant mosquitoes was sequenced, and a simple allele-specific PCR-based assay (AS-PCR) was used to analyze mutations at the population level. The previously reported mutations, V1016I and F1534C, were found with frequencies ranging from 0.04 to 0.41, and 0.56 to 0.71, respectively, in the three cities. Moreover, a novel mutation, at 419 codon (V419L), was found in Ae. aegypti populations from Bello, Riohacha and Villavicencio cities with allelic frequencies of 0.06, 0.36, and 0.46, respectively. Interestingly, the insecticide susceptibility assays showed that mosquitoes from Bello were susceptible to λ-cyhalothrin pyrethroid whilst those from Riohacha and Villavicencio were resistant. A positive association between V419L and V1016I mutations with λ-cyhalothrin resistance was established in Riohacha and Villavicencio. The frequency of the F1534C was high in the three populations, suggesting that this mutation could be conferring resistance to insecticides other than λ-cyhalothrin, particularly type I pyrethroids. Further studies are required to confirm this hypothesis.

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Chronology of sodium channel mutations associated with pyrethroid resistance in Aedes aegypti

Chen

Du²,

Nomura

et al. 2020

Arch Insect Biochem Physiol

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Aedes aegypti is the primary mosquito vector of dengue, yellow fever, Zika and chikungunya. Current strategies to control Ae. aegypti rely heavily on insecticide interventions. Pyrethroids are a major class of insecticides used for mosquito control because of their fast acting, highly insecticidal activities and low mammalian toxicity. However, Ae. aegypti populations around the world have begun to develop resistance to pyrethroids. So far, more than a dozen mutations in the sodium channel gene have been reported to be associated with pyrethroid resistance in Ae. aegypti. Co‐occurrence of resistance‐associated mutations is common in pyrethroid‐resistant Ae. aegypti populations. As global use of pyrethroids in mosquito control continues, new pyrethroid‐resistant mutations keep emerging. In this microreview, we compile pyrethroid resistance‐associated mutations in Ae. aegypti in a chronological order, as they were reported, and summarize findings from functional evaluation of these mutations in an in vitro sodium channel expression system. We hope that the information will be useful for tracing possible evolution of pyrethroid resistance in this important human disease vector, in addition to the development of methods for global monitoring and management of pyrethroid resistance in Ae. aegypti.

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Primer reporte de la mutación F1534C, asociada con resistencia cruzada a DDT y piretroides, en Aedes aegypti de Colombia

Cited by 15 publications

References 20 publications

Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia

Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia

A Point Mutation V419L in the Sodium Channel Gene from Natural Populations of Aedes aegypti Is Involved in Resistance to λ-Cyhalothrin in Colombia

Chronology of sodium channel mutations associated with pyrethroid resistance in Aedes aegypti

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