Chronology of sodium channel mutations associated with pyrethroid resistance in <i>Aedes aegypti</i>

Chen, Mengli; Du, Yuzhe; Nomura, Yasuo; Zhorov, Boris S.; Dong, Ke

doi:10.1002/arch.21686

Cited by 33 publications

(46 citation statements)

References 106 publications

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“…aegypti control is their impact on mosquito populations with high frequencies of alleles that confer resistance to conventional pyrethroids. This is a global challenge [56] that affects many insecticide-based vector control operations [5]. Resistance to pyrethroids is mediated by a range of mechanisms that involve point mutations in specific genes or upregulation of metabolic enzymatic pathways [8].…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

The entomological impact of passive metofluthrin emanators against indoor Aedes aegypti: A randomized field trial

et al. 2021

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Background In the absence of vaccines or drugs, insecticides are the mainstay of Aedes-borne disease control. Their utility is challenged by the slow deployment of resources, poor community compliance and inadequate household coverage. Novel application methods are required. Methodology and principal findings A 10% w/w metofluthrin “emanator” that passively disseminates insecticide from an impregnated net was evaluated in a randomized trial of 200 houses in Mexico. The devices were introduced at a rate of 1 per room and replaced at 3-week intervals. During each of 7 consecutive deployment cycles, indoor resting mosquitoes were sampled using aspirator collections. Assessments of mosquito landing behaviours were made in a subset of houses. Pre-treatment, there were no differences in Aedes aegypti indices between houses recruited to the control and treatment arms. Immediately after metofluthrin deployment, the entomological indices between the trial arms diverged. Averaged across the trial, there were significant reductions in Abundance Rate Ratios for total Ae. aegypti, female abundance and females that contained blood meals (2.5, 2.4 and 2.3-times fewer mosquitoes respectively; P<0.001). Average efficacy was 60.2% for total adults, 58.3% for females, and 57.2% for blood-fed females. The emanators also reduced mosquito landings by 90% from 12.5 to 1.2 per 10-minute sampling period (P<0.05). Homozygous forms of the pyrethroid resistant kdr alleles V410L, V1016L and F1534C were common in the target mosquito population; found in 39%, 24% and 95% of mosquitoes collected during the trial. Conclusions/Significance This is the first randomized control trial to evaluate the entomological impact of any volatile pyrethroid on urban Ae. aegypti. It demonstrates that volatile pyrethroids can have a sustained impact on Ae. aegypti population densities and human-vector contact indoors. These effects occur despite the presence of pyrethroid-resistant alleles in the target population. Formulations like these may have considerable utility for public health vector control responses.

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Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

The entomological impact of passive metofluthrin emanators against indoor Aedes aegypti: A randomized field trial

et al. 2021

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“…5 Aedes aegypti, the yellow fever mosquito, is a remarkably effective vector for numerous important human arbovirus diseases, including dengue fever, yellow fever, Chikungunya, and Zika. In this species, the amino-acid variation associated with pyrethroid resistance include V410L, G923V, L982W, S989P, A1007G, I1011V/M, V1016G/I, T1520I, F1534C/L, and D1763Y (amino-acid positions corresponding to the Musca domestica VGSC model) (see references in Fan et al, 6 Du et al, 7 and Chen et al 8 ). In Latin America, F1534C is the kdr substitution most frequently reported to date.…”

Section: Introductionmentioning

confidence: 99%

A first, naturally occurring substitution at the second pyrethroid receptor of voltage‐gated sodium channel of Aedes aegypti

Itokawa

Furutani

Takaoka

et al. 2021

Pest Management Science

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BACKGROUND Aedes aegypti is a remarkably effective mosquito vector of epidemiologically important arboviral diseases including dengue fever, yellow fever and Zika. The present spread of resistance against pyrethroids, the primary insecticides used for mosquito control, in global populations of this species is of great concern. The voltage‐gated sodium channel (VGSC) in the nervous system is the known target site of pyrethroids in insects. Past studies have revealed several amino‐acid substitutions in this channel that confer pyrethroid resistance, which are known as knockdown resistance (kdr) mutations. RESULTS This study investigated a laboratory colony of Ae. aegypti, MCNaeg, established from larvae collected in Rio de Janeiro, Brazil in 2016. The MCNaeg colony showed strong resistance against pyrethroids without laboratory selection. Of the two VGSC gene haplotypes present within this colony, one harbored three known kdr mutations, V410L, V1016I, and F1534C, and the other harbored only the known F1534C mutation. In latter haplotype, we also found novel amino‐acid substations including V253F. Previous molecular modeling and electrophysiological studies suggest that this residue serves a pyrethroid‐sensing site in the second receptor, PyR2. Our genetical analysis showed that the haplotype harboring V253F and F1534C is associated with equal or slightly stronger resistance than the other triple kdr haplotype to both Type I and Type II pyrethroids. CONCLUSION The novel substitution V253F is potentially involved in pyrethroid resistance in Ae. aegypti. Further studies are needed to elucidate the role of this substitution in the pyrethroid susceptibility of VGSC. © 2021 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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“…Na v s, the targets of pyrethroids, have been characterized in many species 8–10 . In 1989, Loughney et al .…”

Section: Introductionmentioning

confidence: 99%

Unique post‐translational modifications diversify the sodium channels in peach aphid (Myzus persicae [Sulzer])

Wang

Duan

Gao

et al. 2021

Pest Management Science

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BACKGROUND Myzus persicae (Sulzer), a worldwide pest, has caused remarkable damage to agriculture. Among the various control methods, chemical control (especially pyrethroids) is most commonly used. The targets of pyrethroids are voltage‐gated sodium channels (Navs). Unlike those of other insects, all Navs of aphids (including two genes), such as Myzus persicae, are unique. RESULTS In this study, three interlock patterns, I(918)‐F(1014), L(918)‐L(1014), and T(918)‐F(1014), were found at sites 918 and 1014 in the sensitive Myzus persicae strain. Similar to that of other aphids, the Nav of Myzus persicae (MpNav) consisted of two parts, that is MpNav‐I and MpNav‐II, which were embedded with an atypical ‘DENS’ ion selectivity filter and a conventional ‘MFM’ inactivation gate, respectively. MpNav had 11 alternative exons, including two mutually exclusive exons (k and l) and three exons (w, x, and t), which were located in domains I and III, respectively. In addition, various RNA editing events, A503T and V588A, appearing between the connection of domains I and II and the S3 of domain IV, respectively, had been described. CONCLUSION Overall, MpNav was characterized by unique post‐translational regulation mode, 918 and 1014 interlocks, and unusually alternative exons. Our research provides a new perspective on the evolution and variation of insect Navs. © 2021 Society of Chemical Industry

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Chronology of sodium channel mutations associated with pyrethroid resistance in Aedes aegypti

Cited by 33 publications

References 106 publications

The entomological impact of passive metofluthrin emanators against indoor Aedes aegypti: A randomized field trial

The entomological impact of passive metofluthrin emanators against indoor Aedes aegypti: A randomized field trial

A first, naturally occurring substitution at the second pyrethroid receptor of voltage‐gated sodium channel of Aedes aegypti

Unique post‐translational modifications diversify the sodium channels in peach aphid (Myzus persicae [Sulzer])

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