Unique post‐translational modifications diversify the sodium channels in peach aphid (Myzus persicae [Sulzer])

Abstract

BACKGROUND Myzus persicae (Sulzer), a worldwide pest, has caused remarkable damage to agriculture. Among the various control methods, chemical control (especially pyrethroids) is most commonly used. The targets of pyrethroids are voltage‐gated sodium channels (Navs). Unlike those of other insects, all Navs of aphids (including two genes), such as Myzus persicae, are unique. RESULTS In this study, three interlock patterns, I(918)‐F(1014), L(918)‐L(1014), and T(918)‐F(1014), were found at sites 918 and 1014 in the sensitive Myzus persicae strain. Similar to that of other aphids, the Nav of Myzus persicae (MpNav) consisted of two parts, that is MpNav‐I and MpNav‐II, which were embedded with an atypical ‘DENS’ ion selectivity filter and a conventional ‘MFM’ inactivation gate, respectively. MpNav had 11 alternative exons, including two mutually exclusive exons (k and l) and three exons (w, x, and t), which were located in domains I and III, respectively. In addition, various RNA editing events, A503T and V588A, appearing between the connection of domains I and II and the S3 of domain IV, respectively, had been described. CONCLUSION Overall, MpNav was characterized by unique post‐translational regulation mode, 918 and 1014 interlocks, and unusually alternative exons. Our research provides a new perspective on the evolution and variation of insect Navs. © 2021 Society of Chemical Industry

Functional diversity of sodium channel variants in common eastern bumblebee, Bombus impatiens

“…Exon w is located between S2 and S3 in domain I of BiNa v , and exon w has high frequency (92.85%). The same exon has been reported in aphids, Toxoptera citricida and Myzus persicae, the frequency is 92.00% and 80.65%, respectively (Jiang et al, 2017;Wang et al, 2021). The genomic structure of exon w is highly conserved within insects, but not in aphis and bees.…”

Functional Characterization of Double Mutations T929I/K1774N in the Voltage-Gated Sodium Channel of Megalurothrips usitatus (Bagnall) Related to Pyrethroid Resistance

“…Sodium channels from Drosophila melanogaster, Blattella germanica, and Bombus impatiens have all been successfully cloned and functionally expressed in Xenopus oocytes, which promote the study of the mechanism of insecticide resistance. − Previous studies on voltage-gated sodium channel have focused on changes in channel properties by gene alternative splicing, RNA editing, or genes by mutations, which lead to resistance to chemicals by the organisms. − Meanwhile, two pyrethroid binding domains were also predicted: pyrethroid binding site I: domain IIS4–S5 linker, IIS5 and IIIS6; and pyrethroid binding site II: domain IS4–S5 linker, IS5 and IIS6. Pyrethroid binding site I and binding site II are symmetrical in space, whereas the binding directions of the two sites are opposite. , Generally, double or multiple mutations usually cause sodium channels to be less sensitive to pyrethroids than a single mutation.…”

Anti-Aphid Polyketides from Streptomyces sp. SA61

“…The green peach aphid, Myzus persicae (Sulzer), is a polyphagous arthropod pest detrimental to more than 500 plant species from around the world. − M. persicae can cause damage to plants not only through feeding on plant sap, which results in the yellowing and curling of leaves, but also by excreting large amounts of honeydew, which promotes sooty blotch on leaves. The aphid can also transmit about 100 to 120 different plant viruses causing disease. ,− Its characteristics of wide host range, strong reproductive ability, high-speed propagation, and the increase in pesticide resistance lead to severe economic loss and has made M. persicae a global agricultural pest that threatens crop yield and quality of plants. , …”