Susceptibility of Japanese Cyprininae fish species to cyprinid herpesvirus 2 (CyHV-2)

Ito, Takafumi; Maeno, Yukio

doi:10.1016/j.vetmic.2014.01.002

Cited by 26 publications

(29 citation statements)

References 16 publications

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“…Recently, it has been detected in many main culture regions of gibel carp [74] and caused huge economic loss. The experimental infection of indigenous Cyprininae species in Japan, such as ginbuna C. auratus langsdorfii , nagabuna C. auratus buergeri , nigorobuna C. auratus grandoculis and common carp, suggested that Japanese Carassius fish species possess different ability to depress the replication of CyHV-2 [82]. To obtain gynogenetic clones with strong resistance to Ca HV, we evaluated the susceptibilities of a leading variety A + in China, a candidate variety F and wild clones in responsive to Ca HV.…”

Section: Discussionmentioning

confidence: 99%

Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes

et al. 2017

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BackgroundGibel carp is an important aquaculture species in China, and a herpesvirus, called as Carassius auratus herpesvirus (CaHV), has hampered the aquaculture development. Diverse gynogenetic clones of gibel carp have been identified or created, and some of them have been used as aquaculture varieties, but their resistances to herpesvirus and the underlying mechanism remain unknown.ResultsTo reveal their susceptibility differences, we firstly performed herpesvirus challenge experiments in three gynogenetic clones of gibel carp, including the leading variety clone A+, candidate variety clone F and wild clone H. Three clones showed distinct resistances to CaHV. Moreover, 8772, 8679 and 10,982 differentially expressed unigenes (DEUs) were identified from comparative transcriptomes between diseased individuals and control individuals of clone A+, F and H, respectively. Comprehensive analysis of the shared DEUs in all three clones displayed common defense pathways to the herpesvirus infection, activating IFN system and suppressing complements. KEGG pathway analysis of specifically changed DEUs in respective clones revealed distinct immune responses to the herpesvirus infection. The DEU numbers identified from clone H in KEGG immune-related pathways, such as “chemokine signaling pathway”, “Toll-like receptor signaling pathway” and others, were remarkably much more than those from clone A+ and F. Several IFN-related genes, including Mx1, viperin, PKR and others, showed higher increases in the resistant clone H than that in the others. IFNphi3, IFI44-like and Gig2 displayed the highest expression in clone F and IRF1 uniquely increased in susceptible clone A+. In contrast to strong immune defense in resistant clone H, susceptible clone A+ showed remarkable up-regulation of genes related to apoptosis or death, indicating that clone A+ failed to resist virus offensive and evidently induced apoptosis or death.ConclusionsOur study is the first attempt to screen distinct resistances and immune responses of three gynogenetic gibel carp clones to herpesvirus infection by comprehensive transcriptomes. These differential DEUs, immune-related pathways and IFN system genes identified from susceptible and resistant clones will be beneficial to marker-assisted selection (MAS) breeding or molecular module-based resistance breeding in gibel carp.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3945-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes

et al. 2017

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“…) and Prussian carp (Xu et al . ), whereas in a study conducted on Japanese Cyprininae fish species, no mortality was observed in some subspecies of goldfish ( C. auratus langsdorfii , C. auratus buergeri , C. auratus grandoculis ) and in common carp, Cyprinus carpio L. (Ito & Maeno ). Likewise in CEFAS infection trials, CyHV‐2 was not pathogenic for crucian carp (Jeffery et al .…”

Section: Virological Examination Of Eight Crucian Carp and Five Commomentioning

confidence: 91%

New detection of Cyprinid herpesvirus 2 in mass mortality event of Carassius carassius (L.), in Italy

Fichi

Susini

Cocumelli

et al. 2016

Journal of Fish Diseases

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“…Subsequently, reported on the potential efficacy of vaccines derived from formalininactivated cell culture supernatant of CyHV-2-infected GFF cells against HVHN in goldfish. Furthermore, we demonstrated that sensitivity of indigenous Cyprininae fish species in Japan to CyHV-2 is much lower than that of goldfish (Ito & Maeno 2014).…”

Section: Introductionmentioning

confidence: 87%

Effects of experimentally induced infections of goldfish Carassius auratus with cyprinid herpesvirus 2 (CyHV-2) at various water temperatures

Ito

Maeno²

2014

Dis. Aquat. Org.

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In this study, we examined the influence of water temperature on the development of herpesviral haematopoietic necrosis (HVHN) in goldfish Carassius auratus after experimentally induced infection with cyprinid herpesvirus 2 (CyHV-2). In Expt 1, Ryukin goldfish were infected with CyHV-2 by intraperitoneal injection and maintained at 4 different water temperatures. Cumulative mortalities of the 15, 20, 25 and 30°C groups were 10, 90, 90 and 60%, respectively. Therefore, the temperature range of 20-25°C is considered highly permissive for HVHN. One of 6 surviving fish of the 15°C group died after a rapid temperature increase to 25°C at 30 d post infection. All 3 Edonishiki goldfish, co-reared with the surviving Ryukin in tanks where the water temperature was increased from 15 to 25°C, died. In Expt 2, Edonishiki goldfish were exposed to CyHV-2 by bath immersion at 13 or 24°C, resulting in cumulative mortalities of 0 and 87%, respectively, at 28 d post-exposure. No mortality of the surviving Edonishiki in the 13°C treatment was observed when the water temperature was increased to 24°C. In addition, in Expt 2, no mortality was observed in any Ranchu co-reared with CyHV-2-immersed Edonishiki in the group where water temperature was increased from 13 to 24°C, even after re-immersion challenge with CyHV-2. It is interesting to note that CyHV-2 DNA was detected in the kidneys of 4 of the 5 surviving Ranchu co-reared with the CyHV-2-immersed Edonishiki group where the water temperature was increased from 13 to 24°C. Therefore, it is likely that the surviving Edonishiki of the 13°C group were virus carriers. This study indicates that most fish infected with CyHV-2 at 13-15°C acquire resistance to HVHN, but as carriers they are able to infect naïve fish.

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Susceptibility of Japanese Cyprininae fish species to cyprinid herpesvirus 2 (CyHV-2)

Cited by 26 publications

References 16 publications

Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes

Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes

New detection of Cyprinid herpesvirus 2 in mass mortality event of Carassius carassius (L.), in Italy

Effects of experimentally induced infections of goldfish Carassius auratus with cyprinid herpesvirus 2 (CyHV-2) at various water temperatures

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