Survey of Shiga toxigenic Escherichia coli O157 and drug-resistant coliform bacteria from in-line milk filters on dairy farms in the Czech Republic

Čížek, Alois; Dolejská, Monika; Novotná, R; Haas, Doris; Vyskočil, M.

doi:10.1111/j.1365-2672.2007.03602.x

Cited by 17 publications

(4 citation statements)

References 55 publications

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“…These values show the higher probability of antibiotic resistance genes to be spread among animals. The acquisition and spread of antibiotic resistance genes among bacteria that are intimately associated with humans and their domesticated animals are well reported [12]. The dissemination of antibiotic resistance genes among bacterial strains is an increasing problem in infectious diseases.…”

Section: Resultsmentioning

confidence: 99%

Detection of Integrons from Escherichia coli Isolates Obtained from Some Selected Animals in Republic of Korea

Hasan

Tinni

2011

Indian J Microbiol

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Section: Resultsmentioning

confidence: 99%

Detection of Integrons from Escherichia coli Isolates Obtained from Some Selected Animals in Republic of Korea

Hasan

Tinni

2011

Indian J Microbiol

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“…Several studies have documented the drug resistant E. coli and other coliforms in vegetables 29 , poultry 18 , egg 4 , milk 9 and raw meat 35 .…”

Section: Discussionmentioning

confidence: 99%

ANTIMICROBIAL DRUG RESISTANCE IN STRAINS OF Escherichia coli ISOLATED FROM FOOD SOURCES

Rasheed

Thajuddin

Ahamed

et al. 2014

Rev. Inst. Med. trop. S. Paulo

174

106

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A variety of foods and environmental sources harbor bacteria that are resistant to one or more antimicrobial drugs used in medicine and agriculture. Antibiotic resistance in Escherichia coli is of particular concern because it is the most common Gram-negative pathogen in humans. Hence this study was conducted to determine the antibiotic sensitivity pattern of E. coli isolated from different types of food items collected randomly from twelve localities of Hyderabad, India. A total of 150 samples comprising; vegetable salad, raw egg-surface, raw chicken, unpasteurized milk, and raw meat were processed microbiologically to isolate E. coli and to study their antibiotic susceptibility pattern by the Kirby-Bauer method. The highest percentages of drug resistance in isolates of E. coli were detected from raw chicken (23.3%) followed by vegetable salad (20%), raw meat (13.3%), raw egg-surface (10%) and unpasteurized milk (6.7%). The overall incidence of drug resistant E. coli was 14.7%. A total of six (4%) Extended Spectrum β-Lactamase (ESBL) producers were detected, two each from vegetable salads and raw chicken, and one each from raw egg-surface and raw meat. Multidrug resistant strains of E. coli are a matter of concern as resistance genes are easily transferable to other strains. Pathogen cycling through food is very common and might pose a potential health risk to the consumer. Therefore, in order to avoid this, good hygienic practices are necessary in the abattoirs to prevent contamination of cattle and poultry products with intestinal content as well as forbidding the use of untreated sewage in irrigating vegetables.

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“…EHEC isolates belonged to serogroups O26, O103, O157, O128, and O54, the former three being isolated from HUS patients in our study. Čížek et al [79] studied the occurence of EHEC O157 in diary farms in the Czech Republic. EHEC O157 strains harboring stx 1 , stx 2 , eae , and EHEC- hlyA genes were detected in four of 192 in-line filters examined.…”

Section: Discussionmentioning

confidence: 99%

Enterohemorrhagic Escherichia coli as Causes of Hemolytic Uremic Syndrome in the Czech Republic

et al. 2013

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BackgroundEnterohemorrhagic Escherichia coli (EHEC) cause diarrhea-associated hemolytic uremic syndrome (D+ HUS) worldwide, but no systematic study of EHEC as the causative agents of HUS was performed in the Czech Republic. We analyzed stools of all patients with D+ HUS in the Czech Republic between 1998 and 2012 for evidence of EHEC infection. We determined virulence profiles, phenotypes, antimicrobial susceptibilities and phylogeny of the EHEC isolates.Methodology/Principal FindingsVirulence loci were identified using PCR, phenotypes and antimicrobial susceptibilities were determined using standard procedures, and phylogeny was assessed using multilocus sequence typing. During the 15-year period, EHEC were isolated from stools of 39 (69.4%) of 56 patients. The strains belonged to serotypes [fliC types] O157:H7/NM[fliC H7] (50% of which were sorbitol-fermenting; SF), O26:H11/NM[fliC H11], O55:NM[fliC H7], O111:NM[fliC H8], O145:H28[fliC H28], O172:NM[fliC H25], and Orough:NM[fliC H25]. O26:H11/NM[fliC H11] was the most common serotype associated with HUS (41% isolates). Five stx genotypes were identified, the most frequent being stx 2a (71.1% isolates). Most strains contained EHEC-hlyA encoding EHEC hemolysin, and a subset (all SF O157:NM and one O157:H7) harbored cdt-V encoding cytolethal distending toxin. espPα encoding serine protease EspPα was found in EHEC O157:H7, O26:H11/NM, and O145:H28, whereas O172:NM and Orough:NM strains contained espPγ. All isolates contained eae encoding adhesin intimin, which belonged to subtypes β (O26), γ (O55, O145, O157), γ2/θ (O111), and ε (O172, Orough). Loci encoding other adhesins (efa1, lpfA O26, lpfA O157OI-141, lpfA O157OI-154, iha) were usually associated with particular serotypes. Phylogenetic analysis demonstrated nine sequence types (STs) which correlated with serotypes. Of these, two STs (ST660 and ST1595) were not found in HUS-associated EHEC before.Conclusions/SignificanceEHEC strains, including O157:H7 and non-O157:H7, are frequent causes of D+ HUS in the Czech Republic. Identification of unusual EHEC serotypes/STs causing HUS calls for establishment of an European collection of HUS-associated EHEC, enabling to study properties and evolution of these important pathogens.

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Survey of Shiga toxigenic Escherichia coli O157 and drug-resistant coliform bacteria from in-line milk filters on dairy farms in the Czech Republic

Cited by 17 publications

References 55 publications

Detection of Integrons from Escherichia coli Isolates Obtained from Some Selected Animals in Republic of Korea

Detection of Integrons from Escherichia coli Isolates Obtained from Some Selected Animals in Republic of Korea

ANTIMICROBIAL DRUG RESISTANCE IN STRAINS OF Escherichia coli ISOLATED FROM FOOD SOURCES

Enterohemorrhagic Escherichia coli as Causes of Hemolytic Uremic Syndrome in the Czech Republic

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