Survey of clenbuterol in bovine muscle and liver in Ecuador

Espinoza, Wania; Jentzsch, Paul Vargas; Gualpa, Fernando; Andrade, P.; Moreno, Carla; Vaca, I.; Betancourt, Rommel; Medina, Lorena Lorena Valdivia; Enríquez, Dominique; Guijarro-Fuertes, Michelle; Garrido, Patricia; Bravo, Juan; Ulic, Sonia E.; Montalvo, Gemma; Ortega, Fernando; Stolker, Linda; Ramos, Luis A.

doi:10.1080/19393210.2020.1735534

Cited by 6 publications

(6 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is important to note that the clenbuterol molecules showed stability during the cooking process (at 70 C), as some authors had shown previously. 33 In Figure 2 In several countries, including Mexico, 20 China, 27,30 Ecuador, 9 and Italy, 22 beef contaminated with clenbuterol in local markets has been detected, so parts of those contaminated animals may have been used in sausage production. The World Health Organization 11 and European Commission 37 define contaminants as any substance not intentionally added to food or feed for food-producing animals.…”

Section: Detection Of Clenbuterol In Beef Sausage Samplesmentioning

confidence: 99%

“…Substitute species, whether trace adventitious contaminants or products of economically motivated adulteration (EMA), can introduce toxins, pathogens, veterinary residues, or allergens into products. The raw material used in sausage production is usually obtained from registered slaughterhouses where animals are examined by veterinarians 9 . Only raw material from animals whose tissues have been approved for human consumption can be used to elaborate meat products.…”

Section: Introductionmentioning

confidence: 99%

“…The raw material used in sausage production is usually obtained from registered slaughterhouses where animals are examined by veterinarians. 9 Only raw material from animals whose tissues have been approved for human consumption can be used to elaborate meat products.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of clenbuterol residues in beef sausages and its enantiomeric analysis using UHPLC–MS/MS: A risk of unintentional doping in sport field

Velasco-Bejarano

Velasco-Carrillo

Camacho-Frias

et al. 2022

Drug Testing and Analysis

View full text Add to dashboard Cite

Clenbuterol (Clb) can be present in Mexico often but not all over the world in animal tissues and organs, therefore, potentially is derived from animal sources as well. The aims of this study were to develop and validate a method for detecting traces of clenbuterol in beef sausages. A calibration curve showed linearity in the range of 20-500 pg ml À1 . The limit of detection (LOD) and lower limit of quantification (LLOQ) were 5 and 10 pg g À1 , respectively. The analyte recovery was from 95.70% to 100.40% with an intraday relative standard deviation (RSD%) of 0.99%-2.10% and an interday RSD% of 0.54%-2.34%, R 2 = 0.9998. The methodology developed was applied successfully in 15 samples of beef sausage, and 73.3% of the samples tested contained racemic clenbuterol in concentrations between 30 and 471 pg g À1 .The UHPLC-MS/MS method developed combines high sensitivity with good selectivity and short chromatographic run time. Additionally, the enantiomeric analysis of clenbuterol performed in beef sausages showed a 59% for R-(À)-Clb and 41% for S-(+)-Clb. The presence of clenbuterol in beef sausages could represent a risk of unintentional doping in sport field, because the clenbuterol is a banned substance included in the World Anti-Doping Agency's (WADA) list of prohibited substances.

show abstract

Section: Detection Of Clenbuterol In Beef Sausage Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of clenbuterol residues in beef sausages and its enantiomeric analysis using UHPLC–MS/MS: A risk of unintentional doping in sport field

Velasco-Bejarano

Velasco-Carrillo

Camacho-Frias

et al. 2022

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…To the best of our knowledge, clenbuterol hydrochloride (CLB) is usually used as a kind of β-receptor agonist drug. However, on account of its function for reducing muscle fat and increasing muscle protein content, sometimes CLB is added to the feed livestock of undesirable breeders by unscrupulous traders, making it a well-known “lean meat powder.” , …”

Section: Resultsmentioning

confidence: 99%

“…However, on account of its function for reducing muscle fat and increasing muscle protein content, sometimes CLB is added to the feed livestock of undesirable breeders by unscrupulous traders, making it a wellknown "lean meat powder." 48,49 In view of the high stability and excellent luminescence properties of compound 1, luminescence detection explorations toward CLB have been performed. Then, sample 1 and CLB solid were ground adequately and were dispersed in deionized water into a 1 mg/mL suspension separately.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Ultrasensitive Eu-Based MOF Luminescence Sensor for Clenbuterol Visible Recognition

Zhang,

Ding,

Jiao

et al. 2024

Inorg. Chem.

View full text Add to dashboard Cite

Clenbuterol (CLB) as an illegal feed additive may cause a great security risk to food safety. However, convenient and efficient detection means for CLB in practical application remain a formidable challenge. Herein, a stable Eu-based organic framework {[H2N(CH3)2]2[Eu2(ttca)2]·H2O}n (compound 1) (H4ttca = [1,1′:2′,1″-terphenyl]-4,4′,4″,5′-tetracarboxylic acid) has been harvested, exhibiting excellent chemical stability and thermal stability. Luminescence investigation reveals that compound 1 can sensitively and selectively detect CLB without being affected by different components from simulated serum and urine (limit detection: 22.7 nM). Furthermore, sensor 1 can also be applicable to CLB recognition in real swine feeds, presenting excellent anti-interference performance. The good cyclicity of compound 1 endows CLB determination with many advantages: low cost, high stability, and simplicity. Importantly, in view of the indication of the luminescence color (red to blue), test membranes were fabricated and employed for convenient and fast CLB detection, providing a valuable scheme for the visual monitoring of CLB in meat products. This work enriches rare earth metal compounds and luminescence sensor portfolios and breaks the concentration record (nM) for detecting CLB compared with reported complex materials, providing an effective monitoring platform for CLB visually.

show abstract

A novel fluorescence internal filtration immunoassay for the detection of clenbuterol

Peng

Sun

et al. 2021

Eur Food Res Technol

View full text Add to dashboard Cite

Survey of clenbuterol in bovine muscle and liver in Ecuador

Cited by 6 publications

References 35 publications

Detection of clenbuterol residues in beef sausages and its enantiomeric analysis using UHPLC–MS/MS: A risk of unintentional doping in sport field

Detection of clenbuterol residues in beef sausages and its enantiomeric analysis using UHPLC–MS/MS: A risk of unintentional doping in sport field

Ultrasensitive Eu-Based MOF Luminescence Sensor for Clenbuterol Visible Recognition

A novel fluorescence internal filtration immunoassay for the detection of clenbuterol

Contact Info

Product

Resources

About