Foodborne diseases caused by pathogenic bacteria pose a serious threat to human health. Early and rapid detection of foodborne pathogens is urgently needed. The use of biosensors to identify and detect pathogenic bacteria has attracted ample attention because of its high sensitivity, near real-time quantification without enrichment, on-site detection, simple operation and more. As a promising alternative recognition element in biosensors, lectin has been widely studied in bacterial detection because of its high stability and low cost. In...
Au-4MBA@Ag with a strong Raman signal was successfully synthesized, and combination with magnetic separation technology achieved sensitive detection of cTnI.
Sepsis is a systemic inflammatory response syndrome caused by infection. The mortality rate is as high as 30%−50%. Early diagnosis and treatment can significantly improve the mortality of patients with sepsis. Therefore, we have developed a SERS-based magnetic immunoassay method that uses the principle of sandwich method to quantitatively detect Interleukin 6 (IL-6) and Procalcitonin (PCT). In this article, two different Raman reporter molecules are embedded in the middle of the Au@Ag shell and coupled with the tracer antibody to form a SERS immunoprobe. Biotin was coupled with capture antibody to form a sandwich structure when participating in the immune response. Streptavidin and biotin systems have extremely high binding affinity. The sandwich structure is quickly captured by SA magnetic beads and then applied with a magnetic field to enrich the magnetic beads. Finally, simultaneous quantitative detection is achieved by the intensity of the two Raman reporter characteristic peaks on the solution magnetic beads. IL-6 and PCT showed a good relationship between 0−1000 pg ml−1 and 0−20 ng ml−1, respectively, and the limits of detection were 0.54 pg ml−1 and 0.042 ng ml−1, respectively. The recovery rate was between 89.8% and 104.2%, both intra-assay and inter-assay CV were ≤20%. No cross-reaction with C-reactive protein (100 μg ml−1), showing good specificity. This method provides a new technical reference for the clinical detection of sepsis biomarkers.
Acute myocardial infarction (AMI) is the single leading cause of mortality and morbidity worldwide. Heart-type fatty acid-binding protein (H-FABP) and Cardiac troponin I (cTnI), as biomarkers emerging at different stages...
Successfully synthesized Au-4MBA@Ag and Au-XP013@Ag. By detecting the Raman signal on the magnetic beads, the ketamine and amphetamine simultaneous detection was finally realized.
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