A novel fluorescence internal filtration immunoassay for the detection of clenbuterol

Peng, Fei; Li, Beibei; Sun, Shijiao; Mi, Fang; Wang, Ying; Hu, Cunming; Geng, Pengfei; Pang, Lin; Li, Jiutong; Guan, Ming

doi:10.1007/s00217-021-03886-9

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…Separations 2023, 10, 429 2 of 15 So far, various assay approaches, such as high-performance liquid chromatography (HPLC) [8,9], liquid chromatography-mass spectrometry (LC-MS), liquid chromatographytandem mass spectrometry (LC-MS/MS) [10,11], gas chromatography-mass spectrometry (GC-MS) [12,13], electrochemical detection (EC) [14,15], colorimetric ELISA [16,17], and other analytical methods [18,19], have been well applied in detecting β 2 -agonists [1], and it should be quite evident that GC-MS has the comparative advantages of high sensitivity, strong separation ability, and fast real-time analysis among these approaches [20].…”

Section: Introductionmentioning

confidence: 99%

Determination of β2-Agonist Residues in Meat Samples by Gas Chromatography-Mass Spectrometry with N-Doped Carbon Dots in Molecular Sieves

Zhu,

Mou,

Zheng

et al. 2023

Separations

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A simple, effective, and highly sensitive analytical approach was created and applied in this study for the accurate measurement of three β2-agonist residues (clenbuterol, salbutamol, and ractopamine) in meat samples. In the course of the experiment, new adsorbent molecular sieves (ZMS)@nitrogen-doped carbon quantum dots (N-CQDs) composite materials were synthesized with the aid of hydrothermal synthesis. The composite adsorbent materials were prepared and characterized through scanning electron microscopy, transmission electron microscope, X-ray photoelectron spectroscopy, fluorescence, and zeta potential. Four determinants affecting the extraction and elution’s efficiency, such as the amount of adsorbent, the extraction time, desorption time, and the amount of extraction salt, were substantially optimized. The analytes were quantified by gas chromatography–mass spectrometry. Final results of the methodological validation reflected that the ZMS@N-CQDs composite materials were able to adsorb three β2-agonist residues well and had good reproducibility. In the meantime, all analytes indicated good linearity with coefficient of determination R2 ≥ 0.9908. The limit of detection was 0.7–2.0 ng·g−1, the limit of quantification varied from 2.4 to 5.0 ng·g−1, the precision was lower than 11.9%, and the spiked recoveries were in the range of 79.5–97.8%. To sum up, the proposed approach was quite effective, reliable, and convenient for the simultaneous analysis of multiple β2-agonist residues. Consequently, this kind of approach was successfully applied for the analysis of such compounds in meat samples.

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