Surface tailored zein as a novel delivery system for hypericin: Application in photodynamic therapy

Abdelsalam, Ahmed M.; Somaida, Ahmed; Ambreen, Ghazala; Ayoub, Abdallah Mohamed; Tariq, Imran; Engelhardt, Konrad; Garidel, Patrick; Fawaz, Ibrahim; Amin, Muhammed U.; Wojcik, Matthias; Bakowsky, Udo

doi:10.1016/j.msec.2021.112420

Cited by 17 publications

(7 citation statements)

References 74 publications

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“…This suggests that MPPa was homogeneously loaded in entire lipid matrix of SLN. In this regard, MPPa release was dominated by diffusion and dissolution 48 , 49 .…”

Section: Resultsmentioning

confidence: 99%

Improved anticancer efficacy of methyl pyropheophorbide-a–incorporated solid lipid nanoparticles in photodynamic therapy

Yeo

Lee²,

Kim

et al. 2023

Sci Rep

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Photodynamic therapy (PDT) is a promising anticancer treatment because it is patient-friendly and non-invasive. Methyl pyropheophorbide-a (MPPa), one of the chlorin class photosensitizers, is a drug with poor aqueous solubility. The purpose of this study was to synthesize MPPa and develop MPPa-loaded solid lipid nanoparticles (SLNs) with improved solubility and PDT efficacy. The synthesized MPPa was confirmed 1H nuclear magnetic resonance (1H-NMR) spectroscopy and UV–Vis spectroscopy. MPPa was encapsulated in SLN via a hot homogenization with sonication. Particle characterization was performed using particle size and zeta potential measurements. The pharmacological effect of MPPa was evaluated using the 1,3-diphenylisobenzofuran (DPBF) assay and anti-cancer effect against HeLa and A549 cell lines. The particle size and zeta potential ranged from 231.37 to 424.07 nm and − 17.37 to − 24.20 mV, respectively. MPPa showed sustained release from MPPa-loaded SLNs. All formulations improved the photostability of MPPa. The DPBF assay showed that SLNs enhanced the 1O2 generation from MPPa. In the photocytotoxicity analysis, MPPa-loaded SLNs demonstrated cytotoxicity upon photoirradiation but not in the dark. The PDT efficacy of MPPa improved following its entrapment in SLNs. This observation suggests that MPPa-loaded SLNs are suitable for the enhanced permeability and retention effect. Together, these results demonstrate that the developed MPPa-loaded SLNs are promising candidates for cancer treatment using PDT.

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“…This suggests that MPPa was homogeneously loaded in entire lipid matrix of SLN. In this regard, MPPa release was dominated by diffusion and dissolution 48 , 49 .…”

Section: Resultsmentioning

confidence: 99%

Improved anticancer efficacy of methyl pyropheophorbide-a–incorporated solid lipid nanoparticles in photodynamic therapy

Yeo

Lee²,

Kim

et al. 2023

Sci Rep

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“…H. perforatum extract encapsulated in poly(lactic-co-glycolic acid) (PLGA) nanoparticles can successfully and specifically target esophageal squamous carcinoma cells (Amjadi et al 2019). The cellular uptake and phototoxicity of hypericin was enhanced by encapsulation into surface coated zein formulations (Abdelsalam et al 2021). The formation of the inclusion complexes between β-cyclodextrin and constituents of ethanolic extracts of H. perforatum was also confirmed (Kalogeropoulos et al 2008).…”

Section: Introductionmentioning

confidence: 93%

Antimicrobial polyhydroxybutyrate submicron fiber mat loaded with extract of Hypericum perforatum

et al. 2022

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The aim of this work was to prepare a new biodegradable polyhydroxybutyrate (PHB) submicron fiber mat loaded with hypericin-rich Hypericum perforatum raw extract by centrifugal spinning technology, an alternative approach to the traditional method of electrospinning to fabricate nanofibers or microfibers from solutions at high speed and low cost. Hypericins in methanol/acetone extract of H. perforatum were determined by UHPLC-MS/MS and HPLC/PDA. Submicron fiber mats composed of pure PHB or PHB enriched with H. perforatum extract were prepared using a pilot plant demonstrator for the centrifugal spinning technology and characterized by SEM. Singlet oxygen production was quantified by the 1,3-diphenylisobenzofuran (DPIBF) method in hexane. The results proved a significant production of singlet oxygen by the prepared submicron fiber mat. We also found a significant antibacterial activity against the bacterial strain Escherichia coli CCM 5417 by a method in accordance with JIS Z 2801/ISO 22196 standards. The H. perforatum extract-enriched PHB submicron fiber mats showed potential for the development of self-cleaning and antimicrobial air filters.

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“…Half of the amino acid residues are hydrophobic in this peptide. Zein was PEGylated to form NP and further chemical modifications were carried out to increase biocompatibility and stability to use it as a photosensitizer in photodynamic therapy using HepG2 cells [ 47 ]. Two potent apoptotic genes, namely TRAIL and phosphatase and tensin homolog ( PTEN ), were loaded onto a Zein nanoparticle (ZNP) by a phase separation technique to check anti-tumor efficacy in HepG2 cells in vitro and in DEN-treated rats in vivo.…”

Section: Gene Therapy Approaches For Hccmentioning

confidence: 99%

Emerging Therapies for Hepatocellular Carcinoma (HCC)

Chakraborty

Sarkar

2022

Cancers

112

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Hepatocellular carcinoma (HCC) arises from hepatocytes and accounts for 90% of primary liver cancer. According to Global Cancer Incidence, Mortality and Prevalence (GLOBOCAN) 2020, globally HCC is the sixth most common cancer and the third most common cause of cancer-related deaths. Reasons for HCC prognosis remaining dismal are that HCC is asymptomatic in its early stages, leading to late diagnosis, and it is markedly resistant to conventional chemo- and radiotherapy. Liver transplantation is the treatment of choice in early stages, while surgical resection, radiofrequency ablation (RFA) and trans arterial chemoembolization (TACE) are Food and Drug Administration (FDA)-approved treatments for advanced HCC. Additional first line therapy for advanced HCC includes broad-spectrum tyrosine kinase inhibitors (TKIs), such as sorafenib and lenvatinib, as well as a combination of immunotherapy and anti-angiogenesis therapy, namely atezolizumab and bevacizumab. However, these strategies provide nominal extension in the survival curve, cause broad spectrum toxic side effects, and patients eventually develop therapy resistance. Some common mutations in HCC, such as in telomerase reverse transcriptase (TERT), catenin beta 1 (CTNNB1) and tumor protein p53 (TP53) genes, are still considered to be undruggable. In this context, identification of appropriate gene targets and specific gene delivery approaches create the potential of gene- and immune-based therapies for the safe and effective treatment of HCC. This review elaborates on the current status of HCC treatment by focusing on potential gene targets and advanced techniques, such as oncolytic viral vectors, nanoparticles, chimeric antigen receptor (CAR)-T cells, immunotherapy, and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9), and describes future prospects in HCC treatment.

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Surface tailored zein as a novel delivery system for hypericin: Application in photodynamic therapy

Cited by 17 publications

References 74 publications

Improved anticancer efficacy of methyl pyropheophorbide-a–incorporated solid lipid nanoparticles in photodynamic therapy

Improved anticancer efficacy of methyl pyropheophorbide-a–incorporated solid lipid nanoparticles in photodynamic therapy

Antimicrobial polyhydroxybutyrate submicron fiber mat loaded with extract of Hypericum perforatum

Emerging Therapies for Hepatocellular Carcinoma (HCC)

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