Antimicrobial polyhydroxybutyrate submicron fiber mat loaded with extract of <i>Hypericum perforatum</i>

Beran, M.; Horna, Aleš; Voříšek, Viktor; Berkova, Eliska; Korinkova, Radka; Trousil, Vojtěch; Hrubanova, Marketa

doi:10.5010/jpb.2022.49.3.257

Cited by 3 publications

(2 citation statements)

References 77 publications

(62 reference statements)

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“…perforatum, the best-known species of the genus Hypericum, is widely used in traditional medicine for its sedative, analgesic, anthelmintic, anti-inflammatory, and antimicrobial properties [65,66]. The literature data frequently refer to the bactericidal potential of both extracts and essential oils of T. perforatum, which are mainly effective against Grampositive bacteria [65,67,68], but not exclusively [69]. In our study, the pure extract of H. perforatum or embedded in chitosan membranes induced very similar antimicrobial activity against Gram-negative (E. coli and P. aeruginosa) and Gram-positive (S. aureus, MRSA) species.…”

Section: Antimicrobial Properties Of Active Chitosan Membranesmentioning

confidence: 99%

Chitosan Membranes Containing Plant Extracts: Preparation, Characterization and Antimicrobial Properties

Grădinaru¹,

Barbălată-Mândru²,

Enache

et al. 2023

IJMS

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The main strategy of this study was to combine the traditional perspective of using medicinal extracts with polymeric scaffolds manufactured by an engineering approach to fabricate a potential dressing product with antimicrobial properties. Thus, chitosan-based membranes containing S. officinalis and H. perforatum extracts were developed and their suitability as novel dressing materials was investigated. The morphology of the chitosan-based films was assessed by scanning electron microscopy (SEM) and the chemical structure characterization was performed via Fourier transform infrared spectroscopy (FTIR). The addition of the plant extracts increased the sorption capacity of the studied fluids, mainly at the membrane with S. officinalis extract. The membranes with 4% chitosan embedded with both plant extracts maintained their integrity after being immersed for 14 days in incubation media, especially in PBS. The antibacterial activities were determined by the modified Kirby–Bauer disk diffusion method for Gram-positive (S. aureus ATCC 25923, MRSA ATCC 43300) and Gram-negative (E. coli ATCC 25922, P. aeruginosa ATCC 27853) microorganisms. The antibacterial property was enhanced by incorporating the plant extracts into chitosan films. The outcome of the study reveals that the obtained chitosan-based membranes are promising candidates to be used as a wound dressing due to their good physico-chemical and antimicrobial properties.

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Section: Antimicrobial Properties Of Active Chitosan Membranesmentioning

confidence: 99%

Chitosan Membranes Containing Plant Extracts: Preparation, Characterization and Antimicrobial Properties

Grădinaru¹,

Barbălată-Mândru²,

Enache

et al. 2023

IJMS

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“…The results demonstrated strong antibacterial activity of the prepared mats against E. coli. The obtained novel hybrid mat possesses the potential to be used in self-cleaning and antibacterial air filters [123].…”

Section: Plant Extracts Incorporated By Electrospinningmentioning

confidence: 99%

Innovative Bioactive Nanofibrous Materials Combining Medicinal and Aromatic Plant Extracts and Electrospinning Method

Stoyanova,

Nachev,

Spasova

2023

Membranes

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Since antiquity, humans have known about plants as a medicinal cure. Recently, plant extracts are attracting more attention as a result of their natural origin and wide range of desirable features. Nanotechnology’s progress and innovations enable the production of novel materials with enhanced properties for a broad range of applications. Electrospinning is a cutting-edge, flexible and economical technique that allows the creation of continuous nano- and microfibrous membranes with tunable structure, characteristics and functionalities. Electrospun fibrous materials are used in drug delivery, tissue engineering, wound healing, cosmetics, food packaging, agriculture and other fields due to their useful properties such as a large surface area to volume ratio and high porosity with small pore size. By encapsulating plant extracts in a suitable polymer matrix, electrospinning can increase the medicinal potential of these extracts, thus improving their bioavailability and maintaining the required concentration of bioactive compounds at the target site. Moreover, the created hybrid fibrous materials could possess antimicrobial, antifungal, antitumor, anti-inflammatory and antioxidant properties that make the obtained structures attractive for biomedical and pharmaceutical applications. This review summarizes the known approaches that have been applied to fabricate fibrous materials loaded with diverse plant extracts by electrospinning. Some potential applications of the extract-containing micro- and nanofibers such as wound dressings, drug delivery systems, scaffolds for tissue engineering and active food packaging systems are discussed.

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Chitosan/Hyaluronate Complex-Coated Electrospun Poly(3-hydroxybutyrate) Materials Containing Extracts from Melissa officinalis and/or Hypericum perforatum with Various Biological Activities: Antioxidant, Antibacterial and In Vitro Anticancer Effects

Anastasova,

Ignatova,

Manolova

et al. 2024

Polymers

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The present study aimed to fabricate innovative fibrous materials with various biological activities from poly(3-hydroxybutyrate), sodium hyaluronate (HA), chitosan (Ch), Melissa officinalis (MO), Hypericum perforatum (HP) extract, or a combination of both extracts. Electrospinning or electrospinning followed by dip coating and the subsequent formation of a polyelectrolyte complex were the methods used to prepare these materials. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and attenuated total reflection–Fourier transform infrared spectroscopy (ATR–FTIR) were applied for investigating the morphology of materials, their thermal characteristics, and their surface chemical composition. The composition and design of the mats had an influence on the in vitro release behavior of the main bioactive compounds present in the MO and HP extracts incorporated in the materials. It was found that as-created materials comprising a combination of both extracts and a Ch/HA complex exerted higher antioxidant activity than that of (non-)coated MO-containing mats and Ch/HA-coated mats containing HP. The novel materials manifested antibacterial efficacy towards the pathogenic bacteria S. aureus and E. coli, as evidenced by the performed microbiological screening. Furthermore, the mats possessed a great growth inhibitory effect on HeLa cancer cells but had a less pronounced effect on the growth of normal mouse BALB/3T3 fibroblasts. The loading of both extracts in the mats and the formation of coating led to the enhancement of the in vitro anticancer and antibacterial activities of the materials. Thus, the novel materials have potential for use in local cancer therapy as well as for use as wound dressings.

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Antimicrobial polyhydroxybutyrate submicron fiber mat loaded with extract of Hypericum perforatum

Cited by 3 publications

References 77 publications

Chitosan Membranes Containing Plant Extracts: Preparation, Characterization and Antimicrobial Properties

Chitosan Membranes Containing Plant Extracts: Preparation, Characterization and Antimicrobial Properties

Innovative Bioactive Nanofibrous Materials Combining Medicinal and Aromatic Plant Extracts and Electrospinning Method

Chitosan/Hyaluronate Complex-Coated Electrospun Poly(3-hydroxybutyrate) Materials Containing Extracts from Melissa officinalis and/or Hypericum perforatum with Various Biological Activities: Antioxidant, Antibacterial and In Vitro Anticancer Effects

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