Surface reactions of 4-aminothiophenol with heterobifunctional crosslinkers bearing both succinimidyl ester and maleimide for biomolecular immobilization

Xiao, Shou‐Jun; Wieland, Marco; Brunner, Samuel

doi:10.1016/j.jcis.2005.04.014

Cited by 33 publications

(26 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the case of the polyelectrolyte route (cND-PAH particles), [27] the adsorption of PAH is supported by the presence of the 1571 and 1497 cm À1 bands, which correspond to the amides I and II, respectively. The IR spectrum after the introduction of the maleimide moiety [28,29] showed an intense peak at 1700 cm…”

Section: Resultsmentioning

confidence: 99%

Peptide‐Grafted Nanodiamonds: Preparation, Cytotoxicity and Uptake in Cells

et al. 2008

View full text Add to dashboard Cite

Nanodiamonds that were prepared by high pressure/high temperature were functionalized with biomolecules for biological applications. Nanodiamonds (NDs, < or =35 nm) that were coated by silanization or with polyelectrolyte layers were grafted with a fluorescent thiolated peptide via a maleimido function; this led to an aqueous colloidal suspension that was stable for months. These substituted NDs were not cytotoxic for CHO cells. Their capacity to enter mammalian cells, and their localisation inside were ascertained after labelling the nucleus and actin, by examining the cells by confocal, reflected light and fluorescence microscopy.

show abstract

Section: Resultsmentioning

confidence: 99%

Peptide‐Grafted Nanodiamonds: Preparation, Cytotoxicity and Uptake in Cells

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Thus, for label-free methods such as monitoring the intrinsic spontaneous Raman (SR) or stimulating the resonance Raman modes of the analyte (Figure 2), the biomarker must be a pure sample and be isolated from the complex biological sample using extraction techniques such as HPLC or an immunoassay, or the biomarker itself must exhibit vibrational modes that can be uniquely pulled out from the modes inherent in the background media [145]. Bringing these purification steps to the POC often requires immobilization of affinity ligands such as antibodies or DNA aptamers onto well plates, scintillation vials, or glass slides using basic click chemistry [146]. The downside of these ELISA or extraction kit-style designs is the complexity (i.e.…”

Section: Label-free Colloidal Sersmentioning

confidence: 99%

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

et al. 2017

View full text Add to dashboard Cite

Point-of-care (POC) device development is a growing field that aims to develop low-cost, rapid, sensitive in-vitro diagnostic testing platforms that are portable, selfcontained, and can be used anywhere -from modern clinics to remote and low resource areas. In this review, surface enhanced Raman spectroscopy (SERS) is discussed as a solution to facilitating the translation of bioanalytical sensing to the POC. The potential for SERS to meet the widely accepted "ASSURED" (Affordable, Sensitive, Specific, Userfriendly, Rapid, Equipment-free, and Deliverable) criterion provided by the World Health Organization is discussed based on recent advances in SERS in vitro assay development. As SERS provides attractive characteristics for multiplexed sensing at low concentration limits with a high degree of specificity, it holds great promise for enhancing current efforts in rapid diagnostic testing. In outlining the progression of SERS techniques over the past years combined with recent developments in smart nanomaterials, high-throughput microfluidics, and low-cost paper diagnostics, an extensive number of new possibilities show potential for translating SERS biosensors to the POC.

show abstract

“…SGGRGD) hence confirming the presence of the oligopeptides at the surface (Table 1, silane+peptide). Furthermore, the deconvolution of the C 1s spectrum for biofunctionalized surfaces showed an increase in both peaks (C2 /C3) corresponding to the C-N, C-OH and C = O groups derived from peptides and, also, the appearance extra peak due to the peptide characteristic guanadine and O=C-N (binding energy at 288.9 eV) bonds (see table 2, silane+peptide) [46,[52][53][54].…”

Section: X-ray Photoelectron Spectroscopy (Xps)mentioning

confidence: 99%

Study on the use of 3‐aminopropyltriethoxysilane and 3‐chloropropyltriethoxysilane to surface biochemical modification of a novel low elastic modulus Ti–Nb–Hf alloy

et al. 2014

View full text Add to dashboard Cite

A biocompatible new titanium alloy Ti–16Hf–25Nb with low elastic modulus (45 GPa) and the use of short bioadhesive peptides derived from the extracellular matrix have been studied. In terms of cell adhesion, a comparative study with mixtures of short peptides as RGD (Arg‐Gly‐Asp)/PHSRN (Pro‐His‐Ser‐Arg‐Asn) and RGD (Arg‐Gly‐Asp)/FHRRIKA (Phe‐His‐Arg‐Arg‐Ile‐Lys‐Ala) have been carried out with rat mesenchymal cells. The effect of these mixtures of short peptides have already been studied but there are no comparative studies between them. Despite the wide variety of silane precursors available for surface modification in pure titanium, the majority of studies have used aminosilanes, in particular 3‐minopropyltriethoxysilane (APTES). Nevertheless, the 3‐chloropropyltriethoxysilane (CPTES) is, recently, proposed by other authors. Unlike APTES, CPTES does not require an activation step and offers the potential to directly bind the nucleophilic groups present on the biomolecule (e.g., amines or thiols). Since the chemical surface composition of this new alloy could be different to that pure titanium, both organosilanes have been compared and characterized by means of a complete surface characterization using contact angle goniometry and X‐ray photoelectron spectroscopy. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B:495–502, 2015.

show abstract

Surface reactions of 4-aminothiophenol with heterobifunctional crosslinkers bearing both succinimidyl ester and maleimide for biomolecular immobilization

Cited by 33 publications

References 28 publications

Peptide‐Grafted Nanodiamonds: Preparation, Cytotoxicity and Uptake in Cells

Peptide‐Grafted Nanodiamonds: Preparation, Cytotoxicity and Uptake in Cells

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

Study on the use of 3‐aminopropyltriethoxysilane and 3‐chloropropyltriethoxysilane to surface biochemical modification of a novel low elastic modulus Ti–Nb–Hf alloy

Contact Info

Product

Resources

About