Study on the use of 3‐aminopropyltriethoxysilane and 3‐chloropropyltriethoxysilane to surface biochemical modification of a novel low elastic modulus Ti–Nb–Hf alloy

Paredes, Virginia; Salvagni, Emiliano; Rodríguez‐Castellón, Enrique; Gil, F.J.; Manero, J. M.

doi:10.1002/jbm.b.33226

Cited by 24 publications

(14 citation statements)

References 56 publications

(63 reference statements)

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“…Silane modifications have previously been proven to be a suitable approach to enrich a surface with a chemical group of interest, that can subsequently provide a level of control over cellular responses . To date the results from these experiments have been variable, this is due to a lack of knowledge concerning the different parameters namely chain length associated with specific silane chemistry, and the resulting properties of the silane monolayer which is presented to the cells, and a thorough investigation of the resultant material properties at the sub‐micron scale.…”

Section: Discussionmentioning

confidence: 99%

The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells

Chen

Hunt

Fawcett

et al. 2018

J Biomedical Materials Res

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Silane modification has been proposed as a powerful biomaterial surface modification tool. This is the first comprehensive investigation into the effect of silane chain length on the resultant properties of -NH silane monolayers and the associated osteoinductive properties of the surface. A range of -NH presenting silanes, chain length 3-11, were introduced to glass coverslips and characterized using water contact angles, atomic force microscopy, X-ray photoelectron spectroscopy, and Ninhydrin assays. The ability of the variation in chain length to form a homogenous layer across the entirety of the surfaces was also assessed. The osteoinductive potential of the resultant surfaces was evaluated by real-time polymerase chain reaction, immunocytochemistry, and von Kossa staining. Control of surface chemistry and topography was directly associated with changes in chain length. This resulted in the identification of a specific, chain length 11 (CL11) which significantly increased the osteoinductive properties of the modified materials. Only CL11 surfaces had a highly regular nano-topography/roughness which resulted in the formation of an appetite-like layer on the surface that induced a significantly enhanced osteoinductive response (increased expression of osteocalcin, CBFA1, sclerostin, and the production of a calcified matrix) across the entirety of the surface. © 2018 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1862-1877, 2018.

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Section: Discussionmentioning

confidence: 99%

The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells

Chen

Hunt

Fawcett

et al. 2018

J Biomedical Materials Res

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“…22,47 In this regard, APTES has been extensively used for surface functionalization purposes and demonstrated its suitability for the immobilization of bioactive molecules onto Ti 22 and TiNbHf alloy. 48 Moreover, in a recent study we demonstrated that the use of APTES provides a more homogenous layer of biomolecule attachment in both Ti and TiNbHf alloy compared to physisorption. 49 Hence, in the present work APTES was chosen as linker molecule to immobilize the different bioactive molecules onto a recently developed low modulus TiNbHf alloy.…”

Section: One Approach To Improve Osseointegration In Metallic Implantmentioning

confidence: 93%

Tuning Mesenchymal Stem Cell Response onto Titanium–Niobium–Hafnium Alloy by Recombinant Fibronectin Fragments

Herranz-Diez

Mas‐Moruno

Neubauer

et al. 2016

ACS Appl. Mater. Interfaces

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Since metallic biomaterials used for bone replacement possess low bioactivity, the use of cell adhesive moieties is a common strategy to improve cellular response onto these surfaces. In recent years the use of recombinant proteins has emerged as an alternative to native proteins and short peptides owing to the fact that they retain the biological potency of native proteins, while improving their stability. In the present study, we investigated the biological effect of

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“…Aminosilanes are used as precursors in this procedure because they are able to bond with hydroxyl groups which may be induced on the oxidized surface of metals [14,15] and, in particular, may be used to functionalize titanium and its alloys [16][17][18][19]. In turn, the resulting amino groups of the functionalized surface are capable of binding themselves to a wide range of biomolecules [20].…”

Section: Introductionmentioning

confidence: 99%

Development of a versatile procedure for the biofunctionalization of Ti-6Al-4V implants

Rezvanian

Arroyo-Hernández

Ramos

et al. 2016

Applied Surface Science

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HighlightsSurface of Ti-6Al-4V was functionalized by Activated Vapor Silanization (AVS). AVS is proven to be a reliable procedure for functionalizing Ti-6Al-4V samples. The functional layer was characterized by AFM and fluorescence microscopy. The cytocompatibility of the functionalized samples was assessed by cell cultures. The stability of the functional layer under physiological conditions was confirmed.3 Abstract Titanium (Ti) and titanium alloys are among the most-commonly used metallic materials for implantation in the human body for the purpose of replacing hard tissue. Although Ti and its alloys are widely used for such an aim, in implants of a long duration they exhibit some shortcomings due to the loosening of the very implant. This phenomenon is highly dependent on the interaction between the organic tissues and the surface of the implant. In this study, the authors introduce a surface treatment technique for functionalization of the surface of Ti-6Al-4V alloy with amino groups that could help to control this interaction. The functionalized layer was deposited by activated vapor silanization (AVS), which has been proven as a reliable and robust technique with other materials.The resulting biofunctional layers were characterized by atomic force microscopy and fluorescence microscopy, with the optimal conditions for the deposition of a homogeneous film with a high density of amino groups being determined. Additionally, the non-toxic nature and stability of the biofunctional layer were confirmed by cell culturing. The results show the formation of a homogeneous biofunctional amine layer on Ti-6Al-4V alloy that may be used as a platform for the subsequent covalent immobilization of proteins or other biomolecules.

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Study on the use of 3‐aminopropyltriethoxysilane and 3‐chloropropyltriethoxysilane to surface biochemical modification of a novel low elastic modulus Ti–Nb–Hf alloy

Cited by 24 publications

References 56 publications

The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells

The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells

Tuning Mesenchymal Stem Cell Response onto Titanium–Niobium–Hafnium Alloy by Recombinant Fibronectin Fragments

Development of a versatile procedure for the biofunctionalization of Ti-6Al-4V implants

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