Surface plasmon resonance sensor for lysozyme based on molecularly imprinted thin films

Matsunaga, Takateru; Hishiya, Takayuki; Takeuchi, Toshio

doi:10.1016/j.aca.2007.02.072

Cited by 79 publications

(48 citation statements)

References 31 publications

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“…Chen et al 163 also used a small amount of methacrylic acid as copolymer, alongside Nisopropylacrylamide as the major functional monomer, in order to synthesise hydrogels specific for lysozyme and cytochrome C. Matsunaga et al 203 similarly produced lysozyme-imprinted hydrogels films on a sensor surface using an acidic monomer, acrylic acid. In addition to the acidic monomer, the zwitterionic monomer, 2-methacryloylethyl phosphorylcholine, was included in their polymer composition in order to reduce non-specific binding to the hydrogels.…”

Section: Choice Of Functional Monomermentioning

confidence: 99%

“…121,[154][155][156]160,162,180,203 Surface-constrained templates, as required by contact imprinting 127,189,199 and surface confined epitope approaches 117 are probably the most readily adapted to incorporation with transducer surfaces. In these cases a planar thin film results from a casting process, polymer-forming components being confined as a thin film between surfaces, one treated to bind to the polymer (corresponding to the transducer surface) and the other, bearing the template structure, allowing easy release.…”

Section: Polymer Format For Sensing Applicationsmentioning

confidence: 99%

“…Examples of protein analytes detected using SPR sensors include lysozyme 203 and Hepatitis B surface antibody (HBsAb). 194 HbsAb is a biomarker for asymptomatic HBV infection.…”

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confidence: 99%

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The rational development of molecularly imprinted polymer-based sensors for protein detection

et al. 2011

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The detection of specific proteins, as biomarkers of disease, health status, environmental monitoring, food quality, control of fermenters and civil defence purposes means that biosensors for these targets will become increasing more important. Among the technologies used for building specific recognition properties, molecularly imprinted polymers, molecularly imprinted polymers (MIPs) are attracting much attention. In this critical review we describe many of methods used for imprinting recognition for protein targets in polymers and their incorporation with a number of transducer platforms with the aim of identifying the most promising approaches for the preparation of MIP-based protein sensors.2

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Section: Choice Of Functional Monomermentioning

confidence: 99%

Section: Polymer Format For Sensing Applicationsmentioning

confidence: 99%

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The rational development of molecularly imprinted polymer-based sensors for protein detection

et al. 2011

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“…[15] Given the diversity of proteins in terms of physical chemical properties it is unlikely to have the full range of interactions for high affinity binding covered by these simple MIPs. Therefore, the success rate of this probabilistic approach was increased in many cases by focusing on proteins with distinctive properties [8,23] that facilitates the likelihood of obtaining binding sites with both strong and selective interaction with the target. However, for "random"…”

Section: Introductionmentioning

confidence: 99%

Electrosynthesized molecularly imprinted polyscopoletin nanofilms for human serum albumin detection

Stojanović

Erdőssy

Keltai

et al. 2017

Analytica Chimica Acta

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Molecularly imprinted polymers (MIPs) rendered selective solely by the imprinting with protein templates lacking of distinctive properties to facilitate strong target-MIP interaction are likely to exhibit medium to low template binding affinities. While this prohibits the use of such MIPs for applications requiring the assessment of very low template concentrations, their implementation for the quantification of high-abundance proteins seems to have a clear niche in the analytical practice. We investigated this opportunity by developing a polyscopoletin-based MIP nanofilm for the electrochemical determination of elevated human serum albumin (HSA) in urine. As reference for low abundance protein ferritin-MIPs were 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 also prepared by the same procedure. Under optimal conditions, the imprinted sensors gave a linear response to HSA in the concentration range of 20-100 mg/dm 3 , and to ferritin in the range of 120-360 mg/dm 3 . While as expected the obtained limit of detection was not sufficient to determine endogenous ferritin in plasma, the HSA-sensor was successfully employed to analyse urine samples of patients with albuminuria. The results suggest that MIP-based sensors may be applicable for quantifying high abundance proteins in a clinical setting.

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“…В качестве планарных поверхностей используют стекло, слюду, кремний. Метод поверхностного плазмонного резонанса (SPR) был применен для анализа лизоцима [33]. Золотой чип был модифицирован полимером на основе полиакриламида с использование лизоцима в качестве молекулы-шаблона.…”

Section: области применения мип для анализа белков электрохимическиеunclassified

Molecularly imprinted polymers in electro analysis of proteins

Shumyantseva

Булко

Baychorov³

et al. 2015

BIOMED KHIM

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Surface plasmon resonance sensor for lysozyme based on molecularly imprinted thin films

Cited by 79 publications

References 31 publications

The rational development of molecularly imprinted polymer-based sensors for protein detection

The rational development of molecularly imprinted polymer-based sensors for protein detection

Electrosynthesized molecularly imprinted polyscopoletin nanofilms for human serum albumin detection

Molecularly imprinted polymers in electro analysis of proteins

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