Electrosynthesized molecularly imprinted polyscopoletin nanofilms for human serum albumin detection

Abstract

Molecularly imprinted polymers (MIPs) rendered selective solely by the imprinting with protein templates lacking of distinctive properties to facilitate strong target-MIP interaction are likely to exhibit medium to low template binding affinities. While this prohibits the use of such MIPs for applications requiring the assessment of very low template concentrations, their implementation for the quantification of high-abundance proteins seems to have a clear niche in the analytical practice. We investigated this opportunity by developing a polyscopoletin-based MIP nanofilm for the electrochemical determination of elevated human serum albumin (HSA) in urine. As reference for low abundance protein ferritin-MIPs were 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 also prepared by the same procedure. Under optimal conditions, the imprinted sensors gave a linear response to HSA in the concentration range of 20-100 mg/dm 3 , and to ferritin in the range of 120-360 mg/dm 3 . While as expected the obtained limit of detection was not sufficient to determine endogenous ferritin in plasma, the HSA-sensor was successfully employed to analyse urine samples of patients with albuminuria. The results suggest that MIP-based sensors may be applicable for quantifying high abundance proteins in a clinical setting.

Bio‐Electrosynthesis of Vectorially Imprinted Polymer Nanofilms for Cytochrome P450cam

“…Using the nanolithography mode of AFM we found that the thickness increased from 7.9±0.2 nm for the polyscopoletin layer (without Pdx peptide SAM) via 9.2±0.4 nm for the NIP (with underlying Pdx peptide SAM) to 10.4±0.5 nm for the VIP nanofilms (with underlying P450cam template anchored to the Pdx peptide SAM). These results support the nanometer‐scale control over the film thickness and they are close to the values of other electrosynthesized polyscopoletin films ,,…”

Electrosynthesized Molecularly Imprinted Polymer for Laccase Using the Inactivated Enzyme as the Target

“…Whilst thicker films lead to higher loading of the template molecule, thinner films are less stable. However, templates can be easily removed from the thin films …”

“…As a compromise between the lower stability of thin layers and the restriction of target accessibility with increasing film thickness, 10 cyclic voltammetry scans were chosen for the preparation of laccase‐imprinted sensor (Figure ) …”

Advancement in Analytical and Bioanalytical Techniques as a Boon to Medical Sciences

“…An example is the development of a polyscopoletinbased MIP nanofilm for the electrochemical determination of elevated human serum albumin (HSA) in urine. The results suggest that MIP-based sensors may be applicable for quantifying high-abundance proteins in a clinical setting [25].…”