Surface plasmon resonance based sensor for the detection of glycopeptide antibiotics in milk using rationally designed nanoMIPs

Abstract

Glycopeptide antibiotics are known as the last resort for the treatment of serious infections caused by Gram-positive bacteria. The use of milk products contaminated with these antibiotic residues leads to allergic reactions and sensitivity in human. Also, long-term consumption of milk products containing low levels of these antibiotics may cause the relevant bacteria to build up resistance to these last resort antibiotics. Sensitive, rapid and effective quantification and monitoring systems play a key role for their determination in milk products. Hence, molecularly imprinted nanostructures were rationally designed in this work to produce high affinity synthetic receptors to be coupled with a surface plasmon resonance sensor for the analysis of glycopeptide antibiotics in milk samples. The nanoMIP-SPR sensor enabled vancomycin quantification with the LODs of 4.1 ng mL−1 and 17.7 ng mL−1 using direct and competitive assays, respectively. The recoveries rates for two sensor methods ranged in 85–110% with RSDs below 7%. The affinity between the nanoMIP receptors and the target molecule (dissociation constant: 1.8 × 10−9 M) is mostly superior to natural receptors and other synthetic receptors. Unlike other methods commonly employed for the detection of milk contaminants this approach is extremely simple, fast and robust, and do not require pre-sample treatment.

Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella

“…The sensor could differentiate the lowest concentration of DNA (0.002) and negative control ( Figure 8 B) and showed a good reproducibility in a wide investigation range ( Figure 8 C). The LOD was verified based on the linear portion of the saturation graph by calculating three times the standard deviation of the blank response (0.4 × 3 = 1.2 nA) and extrapolating the sensor signal in the linear calibration curve to convert the value to concentration [ 34 ]. The limit of detection was determined to be 0.94 nM with a linear range from 2 to 20 nM, as shown in Figure 8 D.…”

MIPs for commercial application in low-cost sensors and assays – An overview of the current status quo

“…SPR can be considered as one of the most powerful, and therefore popular, readout technologies and has been combined with MIPs for the detection of microorganisms [ 174 , 175 ], amino acids, peptides and proteins [ [176] , [177] , [178] ], dangerous explosives [ [179] , [180] , [181] ], antibiotics [ [182] , [183] , [184] ] and other low-molecular weight compounds [ [185] , [186] , [187] , [188] , [189] ]. Detection upon rebinding of the target is based on changes in electron density at the surface of the sensor chip.…”

Applications of Graphene Quantum Dots in Biomedical Sensors

“…According to our statistical analysis, only a few studies have recently reported on photoelectrochemical (PEC) GQD sensors in comparison to optical and electrochemical sensors. At the same time, various studies have been carried out using SPR sensors for drug monitoring and disease detection [340][341][342][343], but surprisingly only two such studies have reported on the use of GQDs. Given this, despite SPR sensors being optical sensors, we have compiled them in this section.…”