Surface plasmon resonance aptasensor for Brucella detection in milk

Dursun, Ali Doğan; Borsa, Barış Ata; Bayramoğlu, Gülay; Arıca, M. Yakup; Özalp, Veli Cengiz

doi:10.1016/j.talanta.2021.123074

Cited by 24 publications

(15 citation statements)

References 39 publications

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“…The predominant issue with this method is that immobilizing a thiolated aptamer directly on the 2D gold chip surface limits movement and provides less area for aptamers to attach, as compared to a 3D dextran-coated surface which has a much larger number of available sites for aptamer attachment. Thus, the streptavidin–biotin capture technique was chosen to immobilize the aptamer; it is a common technique for immobilizing nucleic acids − since it does not require electrostatic preconcentration of the ligand at the chip surface, allows for less constrained movement of the aptamer to avoid steric hindrances during binding, and allows a higher density of immobilized aptamer due to the 3D dextran matrix which the streptavidin is attached to. While there is a commercial chip (Biacore CM7) available for working with small molecules, it was not used here as it has a higher degree of carboxylation which would increase repulsion of the aptamer from the chip surface due to their like charges.…”

Section: Resultsmentioning

confidence: 99%

Investigation of Charged Small Molecule–Aptamer Interactions with Surface Plasmon Resonance

Froehlich

Haynes

2023

Anal. Chem.

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Investigating the interactions between small, charged molecules and aptamers using surface plasmon resonance (SPR) is limited by the inherent low response of small molecules and difficulties with nonspecific electrostatic interactions between the aptamer, analyte, and sensor surface. However, aptamers are increasingly being used in sensors for small molecule detection in critical areas like healthcare and environmental safety. The ability to probe these interactions through simple, direct SPR assays would be greatly beneficial and allow for the development of improved sensors without the need for complicated signal enhancement. However, these assays are nearly nonexistent in the current literature and are instead surpassed by sandwich or competitive binding techniques, which require additional sample preparation and reagents. In this work, we develop a method to characterize the interaction between the charged small molecule serotonin (176 Da) and an aptamer with SPR using streptavidin−biotin capture and a high-ionic-strength buffer. Additionally, other methods, such as serotonin immobilization and thiol-coupling of the aptamer, were investigated for comparison. These techniques give insight into working with small molecules and allow for quickly adapting a binding affinity assay into a direct SPR sensor.

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Section: Resultsmentioning

confidence: 99%

Investigation of Charged Small Molecule–Aptamer Interactions with Surface Plasmon Resonance

Froehlich

Haynes

2023

Anal. Chem.

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“…Another aptamer with high specificity for B. melitensis cells (B46 aptamer) was used to prepare SPR sensor chips for the sensitive determination of Brucella in magnetic purification eluted samples. This method can rapidly detect B. melitensis contamination in 1 mL milk samples by SPR, with LOD values as low as 27 ± 11 cells [ 72 ].…”

Section: Surface Plasmon Resonance For Bacteria Detectionmentioning

confidence: 99%

Optical Methods for Label-Free Detection of Bacteria

et al. 2022

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Pathogenic bacteria are the leading causes of food-borne and water-borne infections, and one of the most serious public threats. Traditional bacterial detection techniques, including plate culture, polymerase chain reaction, and enzyme-linked immunosorbent assay are time-consuming, while hindering precise therapy initiation. Thus, rapid detection of bacteria is of vital clinical importance in reducing the misuse of antibiotics. Among the most recently developed methods, the label-free optical approach is one of the most promising methods that is able to address this challenge due to its rapidity, simplicity, and relatively low-cost. This paper reviews optical methods such as surface-enhanced Raman scattering spectroscopy, surface plasmon resonance, and dark-field microscopic imaging techniques for the rapid detection of pathogenic bacteria in a label-free manner. The advantages and disadvantages of these label-free technologies for bacterial detection are summarized in order to promote their application for rapid bacterial detection in source-limited environments and for drug resistance assessments.

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“…Surface plasmon resonance (SPR) biosensing attracted considerable interest in recent decades and is a technique that allows label-free detection of various analytes and real-time monitoring of biomolecular events. Ongoing efforts have been made to develop biosensors for disease biomarkers such as nucleic acids (DNA, RNA, microRNA), proteins, antibodies, bacteria, cells, and others [ 3 , 4 , 5 ]. The conventional SPR method requires one binding component to be immobilized on a sensor chip while the other component in the solution is run over the sensor surface [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Strategies for Surface Design in Surface Plasmon Resonance (SPR) Sensing

2023

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Surface plasmon resonance (SPR) comprises several surface-sensitive techniques that enable the trace and ultra-trace detection of various analytes through affinity pairing. Although enabling label-free, sensitive detection and real-time monitoring, several issues remain to be addressed, such as poor stability, non-specific adsorption and the loss of operational activity of biomolecules. In this review, the progress over sensor modification, immobilization techniques and novel 2D nanomaterials, gold nanostructures and magnetic nanoparticles for signal amplification is discussed. The advantages and disadvantages of each design strategy will be provided together with some of the recent achievements.

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Surface plasmon resonance aptasensor for Brucella detection in milk

Cited by 24 publications

References 39 publications

Investigation of Charged Small Molecule–Aptamer Interactions with Surface Plasmon Resonance

Investigation of Charged Small Molecule–Aptamer Interactions with Surface Plasmon Resonance

Optical Methods for Label-Free Detection of Bacteria

Strategies for Surface Design in Surface Plasmon Resonance (SPR) Sensing

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