Surface modification of POSS‐nanocomposite biomaterials using reactive oxygen plasma treatment for cardiovascular surgical implant applications

Abstract

In this study, central composite design (CCD) was used to develop predictive models to optimize operating conditions of plasma surface modification. It was concluded that out of the two process variables, power and duration of plasma exposure, the latter was significantly affecting the surface energy (γ(s) ), chemistry, and topography of polyhedral oligomeric silsesquioxane-poly(carbonate-urea)urethane (POSS-PCU) films. On the basis of experimental data, CCD was used to model the γ(s) using a quadratic modeling of the process variables to achieve optimum surface energy to improve the interaction between endothelial cells (ECs). It was found that optimal water θ for EC adhesion and retention, which was reported 55° from supporting literature (equivalent to γ(s) = 51 mN/m), was easily achievable using the following experimental conditions: (1) power output at 30 W for 75 Sec, (2) 90 W for 40 Sec, and (3) 90 W for 55 Sec in oxygen. In vitro cell culture and metabolic activity studies on optimized films [as in (1)] demonstrate increased adhesion, coverage, and growth of human umbilical vein endothelial cells that were confluent over a shorter time period (<24 H) than controls. Such materials enhanced the EC response and promoted endothelialization on optimized films, thus demonstrating their use as bypass graft materials.

Regeneration of chronic tympanic membrane perforation using 3D collagen with topical umbilical cord serum

“…To create an ideal scaffold for inducing skin regeneration, it should possess excellent biocompatibility, suitable micro-pore structure (e.g., mean pore size of 100-300 m with >90% porosity), controllable biodegradability, and proper mechanical properties [37,38]. Kim et al reported the efficacy of 3D porous chitosan scaffolds for TM regeneration.…”

Functionalized silica nanoparticles as a carrier for Betamethasone Sodium Phosphate: Drug release study and statistical optimization of drug loading by response surface method

“…The "adj R 2 " value corrects the R 2 value for the drug loading percentage and the number of terms. A high value of the The importance and effect of the factors can be determined by the sign and value of the coefficients [32,33]. Thus, the negative coefficient of A (pH) and B (time) indicates an opposite influence on DL%, while the positive coefficient of C (drug/silica) reveals the linear effect.…”

Multifaceted prospects of nanocomposites for cardiovascular grafts and stents

“…50 In a work done by Solouk et al 51 an in vitro study was carried out on human umbilical vein endothelial cells (HUVECs). The nanocomposite efficacy, safety, and compatibility with in vitro cell cultures was investigated.…”

“…In a recent work done by Solouk et al 51 central composite design (CCD) was utilized for developing predictive models. A CCD is an experimental design, mainly used in response surface methodology, for building a second order (quadratic) model and a response variable without requiring the use of a complete three-level factorial experiment.…”

“…From this study, it was observed that among the two process variables (power and duration of plasma exposure), plasma exposure was found to appreciably affect the surface energy (γ s ), chemistry, and topography of POSS-PCU films. 51 Based upon the experimental data, CCD was implemented to model the γ s . This was done by quadratic modeling of process variables for achieving optimum surface energy thereby enhancing the interaction between ECs.…”