Regeneration of chronic tympanic membrane perforation using 3D collagen with topical umbilical cord serum

Searching for a rat model of chronic tympanic membrane perforation: Healing delayed by mitomycin C/dexamethasone but not paper implantation or iterative myringotomy

“…at least eight weeks patency). While this is a different outcome to previous studies with topical mitomycin C, Seonwoo et al [13] only made observations for 1 week and Jang et al [15] for 6 weeks. Further animal experiments are warranted to clarify the efficacy of this mitomycin C/dexamethasone technique in order to resolve opposing views developing in the literature [29,30].…”

“…Dexamethasone is a glucocorticoid with antiinflammatory and immunosuppressant effects [28]. Despite previous reports on the high success rate of using mitomycin C with steroid [13][14][15] in the creation of a chronic TMP animal model, we were unable to replicate the previous reported efficacy of this method. One TMP out of ten stayed patent for seven out of eight weeks, however, this near success rate of 10% is unsatisfactory from a reproducibility point of view.…”

“…Cui et al [14] tested topical mitomycin C (0.2 mg/ml) with hydrocortisone (5 mg/ml) in rats having a success rate of 87% after eight weeks. A recent study [15] applying topical hydrocortisone with ciprofloxacin antibiotic and mitomycin C (0.2 mg/ml) on guinea pigs achieved a success rate of 80% at six weeks.…”

Effect of polycaprolactone/collagen/hUCS microfiber nerve conduit on facial nerve regeneration

“…Human umbilical cord serum (hUCS) contains various growth factors (GFs), such as trans-forming GF-beta, epidermoid GF, acidic and fibroblast GF, platelet-derived GF, hepatocyte GF, vitamin A, substance P, insulin-like growth factor (IGF)-1, nerve growth factor (NGF), fibronectin, and serumanti-proteases, such as ␣-2-macroglobulin [12,13]. Previously, we have reported on the excellent effects of polycaprolactone/collagen nanofiber (PCL/CoNF) combined with hUCS for bone regeneration and tracheal regeneration [14,15].…”

Tracheal regeneration using polycaprolactone/collagen-nanofiber coated with umbilical cord serum after partial resection

“…Umbilical cord serum has many growth factors, and it has been used in studies for regenerative treatment in corneal epithelium or tympanic membrane study [17].…”