2007
DOI: 10.1166/jnn.2007.18116
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Surface Modification of γ-Fe2O3@SiO2 Magnetic Nanoparticles for the Controlled Interaction with Biomolecules

Abstract: Modifying the surface of magnetic nanoparticles (MNPs) to allow for controlled interaction with biomolecules enables their implementation in biomedical applications such as contrast agents for magnetic resonance imaging, labels in magnetic biosensing or media for magnetically assisted bioseparation. In this paper, self-assembly of trialkoxysilanes is used to chemically functionalize the surface of γ-Fe2O3@SiO2 core-shell particles. First, the silane deposition procedure was optimized using infrared analysis i… Show more

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Cited by 28 publications
(13 citation statements)
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“…The strong bands at 634 and 583 cm À1 and a weak band at 443 cm À1 refer to Fe-O vibration of g-Fe 2 O 3 nanoparticles found in both catalyst samples. 36,37 The FT-IR results are in agreement with the XRD and XPS for the characterization of the maghemite phase of g-Fe 2 O 3 nanoparticles.…”
Section: Resultssupporting
confidence: 76%
“…The strong bands at 634 and 583 cm À1 and a weak band at 443 cm À1 refer to Fe-O vibration of g-Fe 2 O 3 nanoparticles found in both catalyst samples. 36,37 The FT-IR results are in agreement with the XRD and XPS for the characterization of the maghemite phase of g-Fe 2 O 3 nanoparticles.…”
Section: Resultssupporting
confidence: 76%
“…When SAV is immobilized on magnetic silica particles without arms, its specific activity for a small probe still can reach about 75% of the free form. 17 Hence, these results supported the effectiveness of the fluorometric assays.…”
supporting
confidence: 62%
“…Many biotin derivatives are effective probes to characterize the immobilization of SAV on solid supports. 6,[15][16][17][18] N-biotin-N 0 -(1-naphthyl)-ethylenediamine (BNE-DA) is successful for homogenous assay of SAV. [4][5][6][7] Herein, we tested the application of BNEDA as a probe for characterizing the immobilization of SAV on MSPs.…”
mentioning
confidence: 99%
“…Different approaches have been used for this purpose, depending on the experimental question addressed. Some methods quantify the total protein content, such as the Bradford and BCA assays [209][210][211] and UV/vis absorption measurements. 212 The protein amount can be measured by the determination of bound proteins or as a difference in the supernatant.…”
Section: Methods Advantages Disadvantagesmentioning
confidence: 99%