Surface modificationviaplasmid-mediated pLAMA3-CM gene transfection promotes the attachment of gingival epithelial cells to titanium sheetsin vitroand improves biological sealing at the transmucosal sites of titanium implantsin vivo

Wang, Jia; He, Xiao-Tao; Xu, Xinyue; Yin, Yuan; Li, Xuan; Bi, Chun‐Sheng; Hong, Yonglong; Chen, Fa‐Ming

doi:10.1039/c9tb01715a

Cited by 16 publications

(22 citation statements)

References 62 publications

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“…Representative images and the number of adherent cells indicated that Ad-LAMA3 treatment accelerates cellular attachment, consistent with the findings of a recent study. 28 However, we did not report any significant difference in day 1 in all of the groups regarding the proportion of wound healing. This is because HD depolymerization had not been fully activated.…”

Section: Discussioncontrasting

confidence: 57%

“…8 , 26 , 27 More research evidence posits that the modification of LAMA3 to the implant surface through a gene-delivery vector promotes the biological sealing of the peri-implant epithelium. 21 , 28 Presently, we utilized the adenovirus vector to overexpress the LAMA3 gene due to its large size. Future studies should be conducted to identify the most pivotal functional fragment of the LAMA3 gene involved in the formation of HDs.…”

Section: Discussionmentioning

confidence: 99%

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Adenovirus-Mediated LAMA3 Transduction Enhances Hemidesmosome Formation and Periodontal Reattachment during Wound Healing

Zhang

Zhen-xuan

et al. 2020

Molecular Therapy - Methods & Clinical Development

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A robust dento-epithelial junction prevents external pathogenic factors from entering connective tissue and could be crucial for periodontal reattachment after periodontal surgery. The junctional epithelium (JE) is attached to the tooth surface through the hemidesmosome (HD) and internal basal lamina, where the primary component is laminin-332. Destruction of the JE leads to the loss of periodontal attachment. Traditional treatments are effective in eliminating local inflammation of the gingiva; however, few directly promote periodontal reattachment and HD formation. Here, we designed a gene-therapy strategy using the adenovirus-mediated human laminin-332 α3 chain (LAMA3) gene (Ad-LAMA3) transduced into a human-immortalized epidermal cell line (HaCaT) to study the formation of HD in vitro . Ad-LAMA3 promoted early adhesion and fast migration of HaCaT cells and increased expression of LAMA3 and type XVII collagen (BP180) significantly. Furthermore, HaCaT cells could facilitate formation of mature HDs after LAMA3 overexpression. In vivo experiments demonstrated that the JE transduced with Ad-LAMA3 could increase expression of LAMA3 and BP180 and “biological sealing” between the tooth and gingival epithelium. These results suggested that adenovirus-mediated LAMA3 transduction is a novel therapeutic strategy that promotes the stability and integration of the JE around the tooth during wound healing.

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Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Adenovirus-Mediated LAMA3 Transduction Enhances Hemidesmosome Formation and Periodontal Reattachment during Wound Healing

Zhang

Zhen-xuan

et al. 2020

Molecular Therapy - Methods & Clinical Development

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“…Atsuta et al reported that laminin332 induces cell migration during PIE formation after Ti implant placement in rats (Atsuta et al, 2005a,b). Wang et al (2019) indicated that the biological sealing around the transmucosal sites of implant could be promoted by the release of plasmid pLAMA3-CM (the C-terminal globular domain of LAMA3) from a titanium surface. These studies also showed that the leading edge of mucosal wound of sulcular epithelium and the underlying connective tissue express laminin332 during wound healing.…”

Section: Discussionmentioning

confidence: 99%

Surfce Functionalized via AdLAMA3 Multilayer Coating for Re-epithelization Around Titanium Implants

Zhang

et al. 2020

Front. Bioeng. Biotechnol.

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The peri-implant epithelium (PIE) forms a crucial seal between the oral environment and the implant surface. Compared with the junctional epithelium (JE), the biological sealing of PIE is fragile, which lacks hemidesmosomes (HDs) and internal basal lamina (extracellular matrix containing laminin332, IBL) on the upper part of the interface. In the study, we aim to prepare a coating with good biocompatibility and ability to immobilize the recombinant adenovirus vector of LAMA3 (AdLAMA3) for promoting the re-epithelization of PIE. The titanium surface functionalized with AdLAMA3 was established via layer-by-layer assembly technique and antibody-antigen specific binding. The biological evaluations including cell adhesion and the re-epithelization of PIE were investigated. The results in vitro demonstrated that the AdLAMA3 coating could improve epithelial cell attachment and cell spreading in the early stage. In vivo experiments indicated that the AdLAMA3 coating on the implant surface has the potential to accelerate the healing of the PIE, and could promote the expression of laminin α3 and the formation of hemidesmosomes. This study might provide a novel approach and experimental evidence for the precise attachment of LAMA3 to titanium surfaces. The process could improve the re-epithelization of PIE.

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“…Chitosan has many applications in this field, as it can be used to coat surfaces in order to increase their biocompatibility and bioactivity [ 130 ]. Chitosan can also be conjugated with other substances, including AgNPs [ 131 , 132 ], hyaluronic acid [ 131 , 133 , 134 ], poly(dopamine) and hydroxyapatite [ 135 ], collagen [ 136 ], silica [ 137 ], and poly(acrylic acid) [ 138 ]. These substances, alongside chitosan, can prevent the surface adhesion of bacteria [ 131 , 132 , 133 , 134 , 138 ], increase the proliferation of cells [ 137 , 138 ], and improve osseointegration [ 134 ], soft tissue integration [ 135 ], and peri-implant tissue attachment [ 136 ].…”

Section: Peri-implantitis and Implant Failuresmentioning

confidence: 99%

Nanoparticles in Dentistry: A Comprehensive Review

2021

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In recent years, nanoparticles (NPs) have been receiving more attention in dentistry. Their advantageous physicochemical and biological properties can improve the diagnosis, prevention, and treatment of numerous oral diseases, including dental caries, periodontal diseases, pulp and periapical lesions, oral candidiasis, denture stomatitis, hyposalivation, and head, neck, and oral cancer. NPs can also enhance the mechanical and microbiological properties of dental prostheses and implants and can be used to improve drug delivery through the oral mucosa. This paper reviewed studies from 2015 to 2020 and summarized the potential applications of different types of NPs in the many fields of dentistry.

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Surface modificationviaplasmid-mediated pLAMA3-CM gene transfection promotes the attachment of gingival epithelial cells to titanium sheetsin vitroand improves biological sealing at the transmucosal sites of titanium implantsin vivo

Abstract: Modification of the transmucosal site of an implant by plasmid-mediated pLAMA3-CM gene transfection is a potential method for future clinical applications.

Cited by 16 publications

References 62 publications

Adenovirus-Mediated LAMA3 Transduction Enhances Hemidesmosome Formation and Periodontal Reattachment during Wound Healing

Adenovirus-Mediated LAMA3 Transduction Enhances Hemidesmosome Formation and Periodontal Reattachment during Wound Healing

Surfce Functionalized via AdLAMA3 Multilayer Coating for Re-epithelization Around Titanium Implants

Nanoparticles in Dentistry: A Comprehensive Review

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