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A robust dento-epithelial junction prevents external pathogenic factors from entering connective tissue and could be crucial for periodontal reattachment after periodontal surgery. The junctional epithelium (JE) is attached to the tooth surface through the hemidesmosome (HD) and internal basal lamina, where the primary component is laminin-332. Destruction of the JE leads to the loss of periodontal attachment. Traditional treatments are effective in eliminating local inflammation of the gingiva; however, few directly promote periodontal reattachment and HD formation. Here, we designed a gene-therapy strategy using the adenovirus-mediated human laminin-332 α3 chain (LAMA3) gene (Ad-LAMA3) transduced into a human-immortalized epidermal cell line (HaCaT) to study the formation of HD in vitro . Ad-LAMA3 promoted early adhesion and fast migration of HaCaT cells and increased expression of LAMA3 and type XVII collagen (BP180) significantly. Furthermore, HaCaT cells could facilitate formation of mature HDs after LAMA3 overexpression. In vivo experiments demonstrated that the JE transduced with Ad-LAMA3 could increase expression of LAMA3 and BP180 and “biological sealing” between the tooth and gingival epithelium. These results suggested that adenovirus-mediated LAMA3 transduction is a novel therapeutic strategy that promotes the stability and integration of the JE around the tooth during wound healing.

Twelve metal coordination compounds with two triazole derivatives, namely, {[Mn(HATr)2](ClO4)2}n (1), [Mn(HATr)3]Cl(ClO4) (2), [Co3(ATr)6(H2O)6](ClO4)6·4.5H2O (3), [Co(HATr)3]Cl(ClO4) (4), [Co2Cl2(HATr)2(H2O)2(CH3OH)2]Cl2·2H2O (5), [Ni3(ATr)6(H2O)6](ClO4)6·4.5H2O (6), [Ni(HATr)3]Cl(ClO4) (7), [Ni2Cl2(HATr)2(H2O)4](ClO4)2·4H2O (8), [Ni2(HATr)2(H2O)6](ClO4)4·2H2O (9), {[Zn(HATr)2](ClO4)2}n (10), [Zn(HATr)3]Cl(ClO4) (11), and {[Cd4(HATr)8](CdCl4)Cl2(ClO4)4}n (12), when HATr = 3-hydrazino-4-amino-1,2,4-triazole and ATr = 4-amino-1,2,4-triazole, were prepared under diverse conditions and structurally characterized. Compounds 1, 10 and 12 exhibit one-dimensional zigzag chain structures; 2, 4, 7, and 11 possess mononuclear structures; 3 and 6 display trinuclear structures, while 5, 8, and 9 feature binuclear structures. Hydrogen bonds link these compounds into three-dimensional structures. The thermal stability and energetic properties also were determined.

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This paper presents a novel guidance law considering the seeker dynamics for manoeuvring targets to achieve short homing time guidance using the Lyapunov method. Based on linear and nonlinear kinematics, a Lyapunov-based guidance law is synthesised to compensate for the seeker’s first-order lag. The closed-form solution of the proposed guidance system is also derived analytically. To implement the proposed guidance law, a Kalman filter algorithm is presented to extract the line-of-sight rate and its higher order derivative. Numerical simulations are carried out to demonstrate the effectiveness of the proposed guidance law under various conditions. Monte Carlo simulations are also performed to test the robustness against measurement noise.

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