Surfce Functionalized via AdLAMA3 Multilayer Coating for Re-epithelization Around Titanium Implants

Abstract

The peri-implant epithelium (PIE) forms a crucial seal between the oral environment and the implant surface. Compared with the junctional epithelium (JE), the biological sealing of PIE is fragile, which lacks hemidesmosomes (HDs) and internal basal lamina (extracellular matrix containing laminin332, IBL) on the upper part of the interface. In the study, we aim to prepare a coating with good biocompatibility and ability to immobilize the recombinant adenovirus vector of LAMA3 (AdLAMA3) for promoting the re-epithelization of PIE. The titanium surface functionalized with AdLAMA3 was established via layer-by-layer assembly technique and antibody-antigen specific binding. The biological evaluations including cell adhesion and the re-epithelization of PIE were investigated. The results in vitro demonstrated that the AdLAMA3 coating could improve epithelial cell attachment and cell spreading in the early stage. In vivo experiments indicated that the AdLAMA3 coating on the implant surface has the potential to accelerate the healing of the PIE, and could promote the expression of laminin α3 and the formation of hemidesmosomes. This study might provide a novel approach and experimental evidence for the precise attachment of LAMA3 to titanium surfaces. The process could improve the re-epithelization of PIE.

Laminin 332-functionalized coating to regulate the behavior of keratinocytes and gingival mesenchymal stem cells to enhance implant soft tissue sealing

“…Moreover, LN332 is deposited into the wound bed by keratinocytes at the leading edge of the epithelium after an injury [ 7 ]. The deposited LN332 repairs the BM and re-establishes the epithelial anchorage to the BM via integrin α6β4 in HDs [ 6 , 34 ]. After implantation surgery, the soft tissue sealing is accompanied by LN332 and integrin α6β4-mediated keratinocyte adhesion, migration, proliferation and PIE attachment to the implant surface via the formation of HDs [ 33 , 34 ].…”

“…The same structures have been reported to exist between the peri-implant epithelium (PIE) and the implant surface [ 5 ]. LN332 is deposited in the wound bed by keratinocytes following implant placement, which mediates keratinocytes adhesion and migration [ 6 , 7 ]. Furthermore, HDs and IBL predominantly reside at the apical portion of the interface between the implant and PIE, which may be associated with insufficient LN332 secretion and insufficient integrin α6β4 expression [ 8 ].…”

Nonthermal Plasma Brush Treatment on Titanium and Zirconia To Improve Periabutment Epithelium Formation

“…LAMA3 coating on the implant surface had the potential to improve the re-epithelization of PIE, thus accelerating the healing time. 41 Multiple peptide coating techniques enhanced epithelial attachment and the establishment of PIE. 42,43 In the present study, NTAPB enhanced the biological behavior of the surfaces by facilitating the formation of IBL and HDs, suggesting the treatment of NTAPB for Ti and ZrO 2 abutment activation to enhance the formation of the periabutment epithelial tissue.…”

Junctional epithelium and hemidesmosomes: Tape and rivets for solving the “percutaneous device dilemma” in dental and other permanent implants

“…For example, a recombinant adenovirus vector for LAMA3 has proven effective in upregulating peri-implant HD expression in rats when delivered on surfaces or as an injectable ( Fig. 5 c) [ 381 , [416] , [417] , [418] ]. Functionalization of surfaces with a cell adhesion peptide motif from a globular domain of laminin332 activates HD formation in vitro [ 382 , [419] , [420] , [421] , [422] ] and in murine [ 423 ] and minipig animal models ( Fig.…”