Surface Markers in Non‐phagocytic Hairy Cell Leukemia

Abstract

By phase contrast and scanning electron microscopy hairy cells were demonstrated in the peripheral blood, bone marrow and spleen of a patient with hairy cell leukemia. Immunofluorescent tests revealed IgD on the surface of 92 per cent, IgG on 76 per cent, IgM on 12 per cent and albumin on 95 per cent of the cells from the spleen. After overnight culture, IgG and albumin were detected on 4 and 6 per cent of the cells respectively, while the number of IgD and IgM positive cells persisted. Fifty‐two per cent of the hairy cells formed rosettes with erythrocytes sensitized with IgG antibodies (EA), whereas 70 per cent formed rosettes after trypsin and protease treatment. The hairy cells did not form rosettes with erythrocytes sensitized with IgM antibodies and complement (EAC), or with sheep and mouse erythrocytes. Cryostat sections of spleen strongly adsorbed EA, whereas no adsorption occurred with EAC or sheep erythrocytes. The hairy cells did not phagocytize latex particles or ingest a strain of yellow staphylococci. The results suggest that hairy cells from this patient probably were of B‐lymphocyte origin.

The Expression of a Human B‐Lymphocyte Antigen on Cells in Different Types of Leukaemia

“…B-cell antigen not identical with Ia-like antigen was also reported by Balch et at. (2, 3) 26,29,30,31,38). In the two cases presented here, the cell surface marker studies suggest a near relation of the hairy cells to B-lymphocytes.…”

Acquired Angioedema and Hypocomplementemia in a Patient with Myelofibrosis

“…A transient increase in Clq paralleled an increase in total hemolytic activity. Nearly normal values were found on days 8 and 15. However, after day 22 the concentration of Clq and the total hemolytic activity returned to values observed before treatment.…”

“…The antinuclear antibody test was also negative. Serum levels of IgG, IgM and IgA were normal as was the distribution of mononuclear cell subpopulations evaluated by various surface markers (8). X-ray examinations of the gastrointestinal tract, lungs, the spine and pelvis were normal.…”

THE MITOGENIC PROPERTIES OF LIPOTEICHOIC ACID FROM STAPHYLOCOCCUS AUREUS

“…Active T lymphocytes (E-active) were demonstrated according to Smith et al (25). Cells with receptors for the Fc part of IgG (EA-rosetting cells) and cells with receptors for complement (EAC-rosetting cells) were counted with a rosette assay as previously described (16). Membranebound immunoglobulins (Ig) on B lymphocytes were detected by a direct immunofluorescence technique (2 I).…”