THE MITOGENIC PROPERTIES OF LIPOTEICHOIC ACID FROM <i>STAPHYLOCOCCUS AUREUS</i>

Abstract--Human neutrophils (PMNs) which have been incubated with lipoteichoic acid (LTA) from group A streptococci generated large amounts of superoxide (O~-chemiluminescence and hydrogen peroxide when challenged with anti-LTA antibodies. Cytochalasin B further enhanced Oy generation. The onset of O2 generation by the LTA-anti-LTA complexes was much faster than that induced by BSA-anti-BSA complexes. LTA-treated PMNs generated much less 02 when challenged with BSA complexes, suggesting that LTA might have blocked, nonspecifically, some of the Fe receptors on PMNs. PMNs treated with LTA-anti-LTA complexes further interacted with bystander nonsensitized PMNs resulting in enhanced O~-generation, suggesting that small numbers of LTA-sensitized PMNs might recruit additional PMNs to participate in the generation of toxic oxygen species. Protelolytic enzyme treatment of PMNs further enhanced the generation of 02 by PMNs treated with LTAanti-LTA. Superoxide generation could also be induced when PMNs and anti-LTA antibodies interacted with target cells (fibroblasts, epithelial cells) pretreated with LTA. This effect was also further enhanced by pretreatment of the target cells with proteases. PMNs incubated with LTA released lysosomal enzymes following treatment with anti-LTA antibodies. The amounts of phosphatase, /3-glucoronidase, Nacetylglucosaminidase, mannosidase, and lysozyme release by LTA-anti-LTA complexes were much smaller than those released by antibody or histone-opsonized streptococci, suggesting that opsonized particles are more efficient lysosomal enzyme releasers. However, since the amounts of O~-generated by the LTA complexes equaled those generated by the opsonized particles, it is assumed that the signals for triggering a respiratory burst and lysosomal enzyme secretion might be different.

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Lipoteichoic acid-antilipoteichoic acid complexes induce superoxide generation by human neutrophils

Ginsburg

Fligiel

Ward

et al. 1988

Inflammation

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THE MITOGENIC PROPERTIES OF LIPOTEICHOIC ACID FROM STAPHYLOCOCCUS AUREUS

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Lipoteichoic acid-antilipoteichoic acid complexes induce superoxide generation by human neutrophils

Lipoteichoic acid-antilipoteichoic acid complexes induce superoxide generation by human neutrophils

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