Surface marker characteristics and Epstein-Barr virus studies of two established North American Burkitt's lymphoma cell lines.

Epstein, Alan L.; Henle, Werner; Henle, Gertrude; Hewetson, John F.; Kaplan, Henry S.

doi:10.1073/pnas.73.1.228

Cited by 56 publications

(11 citation statements)

References 37 publications

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“…At the same time it is fair to add that the importance of these distinctions has been doubted (Dorfman, 1977). BURKITT LYMPHOMA In spite of clinical , epidemiological, immunological (Epstein et al, 1976), andvirological (Zur Hausen, 1975;Andersson et al, 1976;Ziegler et al, 1976) differences between endemic African (Burkitt, 1958) and sporadic African and non-African (O'Conor et al, 1965;Burkitt, 1967) Burkitt lymphomas, their morphological-cytological expressions are very similar (Berard et al, 1969). This tumour has been considered as the most classic example of an undifferentiated ML because of its uniform and monomorphic cellular population without evidence of maturation.…”

Section: Malignant Lymphomas Made Up Exclusively Ormentioning

confidence: 99%

Morphology of lymphatic cells and of their derived tumours.

Rilke

Pilotti

Carbone³

et al. 1978

J Clin Pathol

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Section: Malignant Lymphomas Made Up Exclusively Ormentioning

confidence: 99%

Morphology of lymphatic cells and of their derived tumours.

Rilke

Pilotti

Carbone³

et al. 1978

J Clin Pathol

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“…Ultrastructural, cytochemical, and immunologic studies of the biopsy specimens from patients with diffuse histiocytic lymphomas (DHL) have shown that although some of these malignancies appear to be truly histiocytic by virtue of their lyso- zyme and nonspecific esterase content, others bear monoclonal surface immunoglobulins or are devoid of all distinguishing characterist i c~.~,~~-~~*~~ Some investigators have thus questioned the "histiocytic" nature of these lymphomas and have postulated that they represent a heterogeneous groups of malignancies, the majority of which are derived from the B-lyrnpho~yte.~,~~ In 1974, we reported the successful establishment in continuous culture of cells from the malignant pleural and peritoneal effusions of three patients with DHL. 17 Since then, cell lines from seven additional patients with this diagnosis have been established in continuous cell culture. The neoplastic nature of the first five DHL cell linesl9 and recently of cell lines SU-DHL-6 to 10 (unpublished data) was convincingly shown by intracranial heterotransplantation in the nude, athymic mouse, and for all the cell lines by karyotypic analysis using quinacrine and Giemsa banding technique^.^^ In an attempt to characterize further the malignant cell populations from these ten DHL patients, the cell lines were studied for the presence of surface markers, Epstein-Barr virus (EBV) genomes, cytochemistry, immunoglobulin and lysozyme production, and phagocytic activity.…”

mentioning

confidence: 99%

Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines

Epstein¹,

Levy²,

Kim³

et al. 1978

Cancer

161

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Ten consecutive diffuse histiocytic lymphoma (DHL) cell lines established in our laboratory were studied for the presence of Epstein-Barr virus (EBV) genomes, lysozyme, nonspecific esterase and other cytochemical reactions, phagocytic activity, cytoplasmic immunoglobulin light and heavy chains, and surface receptors to sheep erythrocytes, complement, and the Fc fragment of immunoglobulin. In agreement with previous studies performed on biopsy specimens, our results indicate that the diffuse histiocytic lymphomas, as a histopathologic entity, represent a heterogeneous group of neoplasms, the majority of which are B-lymphocyte in origin. The cell lines appear to fall into three categories based on the following criteria: 1) presence of monoclonal cytoplasmic immunoglobulins (B-lymphocytic type, 6/10 cell lines); 2) presence of non-specific esterase, phagocytic activity, andor lysozyme (histiocytic type, 2/10 cell lines); and 3) absence of all lymphoid and histiocytic cell characteristics (null cell type, 2/10 cell lines). Despite the fact that many of the lymphoma patients had positive serologies to EBV antigens, all of the DHL cell lines were negative for the presence of EBV genomes. Both of the two B-lymphocytic type and one of the two histiocytic type lines tested were susceptible to infection with EBV, as indicated by synthesis of early antigen and also, in a small proportion of the infected cells, of viral capsid antigen. These prototypic DHL cell lines may permit the development of new criteria for the differential diagnosis and treatment of this highly malignant and diverse group of lymphomas.

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“…In the category of lymphoblastic lymphomas all Burkitt type lymphoma cells show surface immunoglobulins in suspension (Binder et al, 1975 ;Epstein et al, 1976;Mann et al, 1976) but only a minority of these tumors actually produce and secrete immunoglobulins (Epstein, 1970;Sherr and Uhr, 1971;Van Furth et al, 1972) and this is also the experience from our immunomorphological studies. The T-cell nature of the convoluted type was assessed by spontaneous rosette formation (Lukes and Collins, 1975;Mann et al, 1975;Stein et al, 1976) and consequently immunoglobulin containing cells are missing in these neoplasms.…”

Section: Discussionmentioning

confidence: 65%