Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines

Abstract

Ten consecutive diffuse histiocytic lymphoma (DHL) cell lines established in our laboratory were studied for the presence of Epstein-Barr virus (EBV) genomes, lysozyme, nonspecific esterase and other cytochemical reactions, phagocytic activity, cytoplasmic immunoglobulin light and heavy chains, and surface receptors to sheep erythrocytes, complement, and the Fc fragment of immunoglobulin. In agreement with previous studies performed on biopsy specimens, our results indicate that the diffuse histiocytic lymphomas, as a histopathologic entity, represent a heterogeneous group of neoplasms, the majority of which are B-lymphocyte in origin. The cell lines appear to fall into three categories based on the following criteria: 1) presence of monoclonal cytoplasmic immunoglobulins (B-lymphocytic type, 6/10 cell lines); 2) presence of non-specific esterase, phagocytic activity, andor lysozyme (histiocytic type, 2/10 cell lines); and 3) absence of all lymphoid and histiocytic cell characteristics (null cell type, 2/10 cell lines). Despite the fact that many of the lymphoma patients had positive serologies to EBV antigens, all of the DHL cell lines were negative for the presence of EBV genomes. Both of the two B-lymphocytic type and one of the two histiocytic type lines tested were susceptible to infection with EBV, as indicated by synthesis of early antigen and also, in a small proportion of the infected cells, of viral capsid antigen. These prototypic DHL cell lines may permit the development of new criteria for the differential diagnosis and treatment of this highly malignant and diverse group of lymphomas.

Clinical and biological relevance of single-nucleotide polymorphisms and acquired somatic mutations of the BCL6 first intron in follicular lymphoma

“…Nuclear extracts were prepared by the method of Schreiber et al 22 and were obtained from 2 t(14;18) þ higher grade transformed FL cell lines (DHL16, RL4). 23,24 Binding reactions were performed in a total volume of 10 ml containing 2 ml of extract, 2 ml of 5 Â binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl (pH 7.5) and 0.25 mg/ml poly(dI-dC)/ poly(dI-dC); Promega, Madison, WI) and 1 ml of double stranded, 32 P-end labeled probes. Incubations were carried out for 20 min at 201C and the resulting complexes were resolved on 4% nondenaturating polyacrylamide gels in 0.5 Â Tris-Borate EDTA.…”

Delayed expression of apoptosis in human lymphoma cells undergoing low-dose taxol-induced mitotic stress

“…DoHH2 was a kind gift from Dr JC Kluin-Nelemans [Leiden, The Netherlands; (Kluin-Nelemans et al, 1991)], and SU-DHL-4 was provided by Professor FE Cotter (Epstein et al, 1978). DoHH2 was routinely maintained in RPMI 1640 supplemented with 5% FCS.…”

Microarray Analysis of B-Cell Lymphoma Cell Lines with the t(14;18)

“…Stanford University diffuse histiocytic lymphoma (SUDHL) cell lines SUDHL-4 and SUDHL-6 25 were generously provided by Dr. Lynn Sorbara, National Cancer Institute, National Institutes of Health, Bethesda, MD. Karpas 422, 26 Ontario Cancer Institute (OCI)-LY1, 27 and NCEB-1 28 were kindly provided by Dr. Neil Berinstein, University of Toronto, Ontario, Canada.…”

“…75 The authors of this study also speculated that a structural loss of HLA class I and II gene expression might contribute to escape of aggressive B-cell lymphomas from immune surveillance. All four of the DLBCL cell lines used for this study were derived from extranodal lymphomas, 25,27 and one of the cell lines (SUDHL-6) has been reported to have a 6p deletion. 30 In summary, this study demonstrates the utility of the combination of cDNA microarray analysis and quantitative real-time RT-PCR for rapid assessment of the transcriptional changes that characterize t(14;18)-positive lymphoma cell lines, and also for the identification of novel genes that could act in concert with deregulated BCL-2 in the genesis and progression of t(14;18)-positive NHLs.…”