Surface Immobilization of Bioactive Molecules on Polyurethane for Promotion of Cytocompatibility to Human Endothelial Cells

Abstract

Cytocompatible polymers are of increasing importance for tissue engineering scaffolds and implanted devices. Polyurethane (PU) membranes were modified by grafting polymerization of methacrylic acid (MAA) initiated by UV light and further covalent immobilization of gelatin or arginine‐glycine‐aspartic (RGD) peptide using 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride as a condensing agent. The immobilization of gelatin or RGD peptide was confirmed by FTIR attenuated total reflection (ATR) spectroscopy and X‐ray photoelectron spectroscopy (XPS). The results for endothelial cells cultured in vitro proved that the PU membrane modified with gelatin or RGD peptide had better cytocompatibility than the control PU or the PMAA grafted PU membrane. ATR‐FTIR spectra of PU‐graft‐PMAA (a), PU‐graft‐PMAA‐graft‐RGD (b), and their subtraction result (c). The PMAA grafting degree is 21.56 × 10−2 mg/cm2.magnified imageATR‐FTIR spectra of PU‐graft‐PMAA (a), PU‐graft‐PMAA‐graft‐RGD (b), and their subtraction result (c). The PMAA grafting degree is 21.56 × 10−2 mg/cm2.

Clear castable polyurethane elastomer for fabrication of microfluidic devices

“…For example, poly(methacrylic acid) has been grafted onto polyurethane surface by UV irradiation followed by immobilization of gelatin or RGD peptide for better cytocompatibility. 22 …”

In Vivo Biocompatibility of Sulfonated PEO‐grafted Polyurethanes for Polymer Heart Valve and Vascular Graft

“…Thus, a variety of approaches have been developed to enhance the biocompatibility of PUs by either surface or bulk modifications (2,3). Methods include (i) biological modification with endothelial cell seeding (4); (ii) utilization of anticoagulant agents, such as heparin (5); and (iii) physical or chemical treatments, including poly(ethylene glycol) (6), phospholipid polymer (7), and sulfonated poly(ethylene oxide) (PEO) (8).…”

Endothelial Cells Adhesion on Modified Polyurethane Surface as the Way to Fabricate a Novel Material for Cardiosurgery

“…The change in the cell shape was expected as the positive premise of the cells anchorage to the surface via integrin-mediated mechanism [63][64][65][66] . However, the density of HUVEC should be higher.…”

“…However, the density of HUVEC should be higher. It was shown that it is possible to obtain a tight monolayer of HUVEC on a PU grafted with gelatin 64 and on modified titanium surface 67 . Besides, obtaining a tight cell coating is crucial because of thrombogenicity of collagen.…”