Surface functionalization by poly-acrylic acid plasma-polymerized films for microarray DNA diagnostics

Surface Treatments and Functional Coatings for Biocompatibility Improvement and Bacterial Adhesion Reduction in Dental Implantology

“…Different precursors and plasma excitation methods, process parameters and reactor geometries influence the plasma polymerization process and lead to a diversity of plasma-chemical reactions and thus to different film properties [58]. The low pressure plasma processes are the most widely used: microwave (2.45 GHz) [59] or capacitively coupled radio frequency (13.56 MHz) [59][60][61][62][63][64][65] activated [60], in planar parallel electrode plates geometry [63][64][65], in steel chamber [59,[63][64][65][66] or glass tubes [62,67,68] reactors, applying a continuous [61,67] or pulsed plasma discharge [59,[63][64][65]69].…”

“…This goal could be achieved through a high degree of cross-linking, but the consequent chains high fragmentation at high discharge power determinates a strong decrease of -COOH groups density. Notwithstanding, it was demonstrated that a finely tuned pulsed RF plasma discharge allows obtaining a high surface density of carboxylic groups (10 15 -10 16 -COOH/cm 2 ), stable in aqueous solutions, able to covalently graft NH 2 terminated c-DNA probes, complementary to Listeria monocytogenes oligonucleotides, for microarray diagnostics [63] and proteins (Protein A) if deposited on porous silicon matrices [64]. Moreover, when applied to polypropylene hernia-repair meshes [65], PPAAc can be successfully loaded with silver nanoparticles that were shown to be moderately released in physiological media.…”

Effect of plasma surface functionalization on preosteoblast cells spreading and adhesion on a biomimetic hydroxyapatite layer formed on a titanium surface

“…Intense bands at 1119 and 1021, 564 cm −1 , and a weak band at 975 cm −1 can be observed in (b; HAp/Ti), (c; COOH/HAp/Ti) and (d; NH 2 /HAp/Ti), which correspond to the P O stretching vibration modes, and the bands at 605 and 564 cm −1 correspond to the O P O bending mode [22]. The peak at 1713 cm −1 in (c; COOH/HAp/Ti) corresponds to the carboxyl group of PPAAc, and the peak at 3200-3350 cm −1 and 1630 cm −1 in (d; NH 2 /HAp/Ti) corresponds to the amine group on the PPAAm thin film present on the HAp/Ti substrate [23,24]. These results show that the chemical surface functionality of interest was present on the modified HAp/Ti substrates, respectively.…”

A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves

“…In order to reach a thickness of 100 nm, the following parameters are selected: (a) D.C.% = 10; t on = 10 ms; t off = 90 ms; (b) Power RF = 200 W; (c) deposition time 15 min. Before deposition an Ar etching step has been performed, in order to clean and activate the surface [29]. …”