Surface-enhanced Raman spectroscopy of the catecholamine neurotransmitters and related compounds

Lee, Nam Soo; Hsieh, You Zung.; Paisley, R. F.; Morris, Michael D.

doi:10.1021/ac00156a014

Cited by 100 publications

(76 citation statements)

References 26 publications

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“…Dopamine beta hydroxylase, an enzyme involved into the synthesis of the neurotransmitters dopamine and norepinephrine, has been shown to be present in DCs [83]. Amino acid peaks found in DCs are also present in spectra of dopamine and norepinephrine [84] suggesting the presence of these neurotransmitters in DCs and their contribution to SERS spectra. To confirm the validity of this hypothesis, UDCs and DCs were fluorescently immune-stained for dopamine and the results are shown in Fig.…”

Section: Functionalised Sers Nanoparticle-probesmentioning

confidence: 99%

Gold nanoparticles explore cells: Cellular uptake and their use as intracellular probes

Huefner

Septiadi

Wilts

et al. 2014

Methods

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Understanding uptake of nanomaterials by cells and their use for intracellular sensing is important for studying their interaction and toxicology as well as for obtaining new biological insight. Here, we investigate cellular uptake and intracellular dynamics of gold nanoparticles and demonstrate their use in reporting chemical information from the endocytotic pathway and cytoplasm. The intracellular gold nanoparticles serve as probes for surface-enhanced Raman spectroscopy (SERS) allowing for biochemical characterisation of their local environment. In particular, in this work we compare intracellular SERS using non-functionalised and functionalised nanoparticles in their ability to segregate different but closely related cell phenotypes. The results indicate that functionalised gold nanoparticles are more efficient in distinguishing between different types of cells. Our studies 2 pave the way for understanding the uptake of gold nanoparticles and their utilisation for SERS to give rise to a greater biochemical understanding in cell-based therapies.

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Section: Functionalised Sers Nanoparticle-probesmentioning

confidence: 99%

Gold nanoparticles explore cells: Cellular uptake and their use as intracellular probes

Huefner

Septiadi

Wilts

et al. 2014

Methods

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“…Since the first discovery of surface enhanced Raman scattering ͑SERS͒ of pyridine on the electrochemically roughened Ag electrode by Fleischmann et al in 1974, 1 SERS has attracted substantial interest because of its potential applications in trace analysis, [2][3][4][5] nanoscience, [6][7][8][9][10] and biological science. [11][12][13][14] Currently, there are two separated mechanisms to describe the overall SERS effect: electromagnetic effect ͑EM͒ and chemical effect ͑CM͒.…”

Section: Introductionmentioning

confidence: 99%

Surface enhanced Raman scattering of p-aminothiophenol self-assembled monolayers in sandwich structure fabricated on glass

Wang

Chen

Dong

et al. 2006

The Journal of Chemical Physics

106

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A sandwich structure consisting of Ag nanoparticles (NPs), p-aminothiophenol (p-ATP) self-assembled monolayers (SAMs), and Ag NPs was fabricated on glass and characterized by surface enhanced Raman scattering (SERS). The SERS spectrum of a p-ATP SAM in such sandwich structure shows that the electromagnetic enhancement is greater than that on Ag NPs assembled on glass. The obtained enhancement factors (EF) on solely one sandwich structure were as large as 6.0±0.62×104 and 1.2±0.62×107 for the 7a and 3b(b2) vibration modes, respectively. The large enhancement effect of p-ATP SAMs is likely a result of plasmon coupling between the two layers of Ag NP (localized surface plasmon) resonance, creating a large localized electromagnetic field at their interface, where p-ATP resides. Moreover, the fact that large EF values (∼1.9±0.7×104 and 9.4±0.7×106 for the 7a- and b2-type vibration modes, respectively) were also obtained on a single sandwich structure of Au NPs∕p-ATPSAMs∕Ag NPs in the visible demonstrates that the electromagnetic coupling does not exist only between Ag NPs but also between Au and Ag NPs. The lower EF values on Au-to-Ag NPs compared to those on Ag-to-Ag NPs demonstrate that the Au-to-Ag coupling must be less effective than the Ag-to-Ag coupling for the induction of SERS in the visible.

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“…Here we use EC-SERS detection of dopamine as an example [31]. The SERS spectrum in the aromatic ring stretching region of dopamine in a pH 7.2 buffer is shown in the inset of Figure 9.11a.…”

Section: Ec-sers As a Methods To Improve The Detection Sensitivity Of mentioning

confidence: 99%

Electrochemical SERS and its Application in Analytical, Biophysical and Life Science

Ren

Cui

et al. 2010

Surface Enhanced Raman Spectroscopy

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Raman spectroscopy, as a vibrational spectroscopy, can record fingerprint spectra from electrodes and provide much insight into a variety of surface and interfacial processes at the molecular level: for example, qualitatively determining surface bonding, conformation and orientation. Raman spectroscopy invariably uses lasers from the ultraviolet (UV) to the near infrared (NIR). More importantly, the technique can be applied in situ to investigate solid-liquid, solid-gas and solid-solid interfaces of both fundamental and practical importance. The technique can be used flexibly to study porous electrode materials of high surface area, to which many surface techniques are not applicable. Therefore, Raman spectroscopy is among the most promising methods for use in electrochemistry. The major disadvantage of Raman spectroscopy is its very low detection sensitivity. However, surface-enhanced Raman scattering (SERS) can improve the sensitivity significantly by several orders of magnitude for roughened surfaces of many metals including noble and transition metals.This chapter will first introduce some fundamental background and features of electrochemical surface-enhanced Raman scattering (EC-SERS), followed by a detailed description of the experimental setup for electrochemical Raman spectroscopy and preparation of SERS substrates. The emphasis will be on how to obtain reliable information of very sensitive bio-related systems. Some examples, varying from a model molecule of benzene to real biomolecules, such as dopamine, NADH, DNA and cytochrome c (cyt c), will be shown to demonstrate how to apply EC-SERS for bio-related application. Finally, prospects and further developments of EC-SERS application in bio-related systems will be discussed. Electrochemical Surface-Enhanced Raman SpectroscopyThe first SERS spectra were obtained from an electrochemical cell when Fleischmann et al. tried to roughen the Ag electrode in order to increase the surface area and hence the number of adsorbed pyridine molecules on the laser spot [1]. This phenomenon was soon demonstrated to be due to the surface enhancement effect

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Surface-enhanced Raman spectroscopy of the catecholamine neurotransmitters and related compounds

Cited by 100 publications

References 26 publications

Gold nanoparticles explore cells: Cellular uptake and their use as intracellular probes

Gold nanoparticles explore cells: Cellular uptake and their use as intracellular probes

Surface enhanced Raman scattering of p-aminothiophenol self-assembled monolayers in sandwich structure fabricated on glass

Electrochemical SERS and its Application in Analytical, Biophysical and Life Science

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