2019
DOI: 10.3390/ma13010149
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Surface Enhanced CdSe/ZnS QD/SiNP Electrochemical Immunosensor for the Detection of Mycobacterium Tuberculosis by Combination of CFP10-ESAT6 for Better Diagnostic Specificity

Abstract: In this study, an electrochemical immunosensor was introduced for the detection of tuberculosis (TB) via utilization of a modified electrode containing a quantum dot (CdSe/ZnS QD) and functionalized silica nanoparticles (SiNPs) on screen-printed carbon electrode (SPCE) CdSe/ZnS QD/SiNPs/SPCE, by employing indirect enzyme-linked immunosorbent assay (ELISA). Here, the fabricated electrode was linked to the biocatalytic action of enzyme catalase through antigen–antibody binding for the detection of the antigen (C… Show more

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Cited by 25 publications
(24 citation statements)
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“…The results also show that the electroactive surface area of SPCE/MPA-Fe 3 O 4 /NCC/CTAB was increased by approximately 54.5% compared to the bare SPCE. This increment shows the ability of the developed electrochemical for providing a wide area platform [32] for DNA immobilization; thus, it can influence the sensitivity of detection [18,38]. Furthermore, the linearity of the plots has suggested that the electron transfer occurred was due to the diffusion-controlled electron transfer process [27,39].…”
Section: Electrochemical Characterizationmentioning
confidence: 88%
“…The results also show that the electroactive surface area of SPCE/MPA-Fe 3 O 4 /NCC/CTAB was increased by approximately 54.5% compared to the bare SPCE. This increment shows the ability of the developed electrochemical for providing a wide area platform [32] for DNA immobilization; thus, it can influence the sensitivity of detection [18,38]. Furthermore, the linearity of the plots has suggested that the electron transfer occurred was due to the diffusion-controlled electron transfer process [27,39].…”
Section: Electrochemical Characterizationmentioning
confidence: 88%
“…Bakhori et al, for the first time, developed an electrochemical immunosensor based on CdSE/ZnS quantum dots (QD) and silica nanoparticles (SiNPS) for Mycobacterium tuberculosis detection [ 41 ]. Screen-printed carbon electrodes were modified with CdSE/ZnS (QD) and SiNPs to amplify the detection signal as well as to increase the selectivity of the sensor.…”
Section: Pathogen Detection With Electrochemical Biosensorsmentioning
confidence: 99%
“…Weiner Kaufmann, 2020Ag85A, PPE, and pfkB Multistage DNA vaccine, A39 Su et al, 2017Ag85C, MPT5, and HspX rBCG-CMX da Costa et al, 2017 Lipo-AE vaccine formulation with the PolyIC adjuvant Diogo et al, 2019 ESAT-6, CFP-10, TB10.3, AlaDH, Ag85B, PstS1, andHspX ELISPOT assay Chiappini et al, 2012 38kD, ESAT-6, and CFP-10 Multiple-antigen detection assay Dai et al, 2017 CFP-10 and ESAT-6 Electrochemical-based SPCE Bakhori et al, 2020 ESAT-6, 6-kDa early secretory antigenic target; CFP, culture filtrate protein; rBCG, recombinant bacille Calmette-Guérin; ELISPOT, enzyme-linked immunospot assay; CMX, Ag85C-MPT51-HspX fusion protein; SPCE, screen-printed carbon electrode. For further details on Mycobacterium tuberculosis (Mtb) proteins, see the database: http://www.tbdb.org and http://tuberculist.epfl.ch immune response to the Ag85B antigen, but a weak immune response to ESAT-6 (Diogo et al, 2019).…”
Section: Id93mentioning
confidence: 99%