DNA Electrochemical Biosensor Based on Iron Oxide/Nanocellulose Crystalline Composite Modified Screen-Printed Carbon Electrode for Detection of Mycobacterium tuberculosis

Abstract

Death from tuberculosis has resulted in an increased need for early detection to prevent a tuberculosis (TB) epidemic, especially in closed and crowded populations. Herein, a sensitive electrochemical DNA biosensor based on functionalized iron oxide with mercaptopropionic acid (MPA-Fe3O4) nanoparticle and nanocellulose crystalline functionalized cetyl trimethyl ammonium bromide (NCC/CTAB) has been fabricated for the detection of Mycobacterium tuberculosis (MTB). In this study, a simple drop cast method was applied to deposit solution of MPA-Fe3O4/NCC/CTAB onto the surface of the screen-printed carbon electrode (SPCE). Then, a specific sequence of MTB DNA probe was immobilized onto a modified SPCE surface by using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling mechanism. For better signal amplification and electrochemical response, ruthenium bipyridyl Ru(bpy)32+ was assigned as labels of hybridization followed by the characteristic test using differential pulse voltammetry (DPV). The results of this biosensor enable the detection of target DNA until a concentration as low as 7.96 × 10−13 M with a wide detection range from 1.0 × 10−6 to 1.0 × 10−12 M. In addition, the developed biosensor has shown a differentiation between positive and negative MTB samples in real sampel analysis.

Screen-printed electrode-based biosensors modified with functional nucleic acid probes and their applications in this pandemic age: a review

“…4A, a specific sequence of Mycobacterium tuberculosis (MTB) DNA probe was immobilized on the modified SPCE surface using the EDC/NHS coupling mechanism. 74 In this work, the immobilization platform was firstly incorporated by negatively charged 3-mercaptopropionic acid (MPA)-Fe 3 O 4 and positively charged nanocellulose crystalline/cetyl trimethyl ammonium bromide (NCC/CTAB) through ionic interaction as a matrix for conductivity enhancement, promoting electron transfer and subsequently acting to immobilize the DNA probe. Although the detection mechanisms are already known, the use of nanoparticles = polymer composites on the SPCE with Ru(bpy) 3 2+ as an electrochemical indicator in the detection of MTB assay is new.…”

A Comprehensive Review on Biopolymer Mediated Nanomaterial Composites and Their Applications in Electrochemical Sensors

“…Iron oxide/nanocellulose crystalline composite deposited on a screen-printed carbon electrode was used to detect Mycobacterium tuberculosis (TB) [113] . This iron oxide/nanocellulose composite has low discernment at 7.96 × 10 −13 M for concentrations in the 1.0 × 10 −6 to 1.0 × 10 −12 M range.…”