Surface engineering of titanium alloy substrates with multilayered biomimetic hierarchical films to regulate the growth behaviors of osteoblasts

Yang, Weihu; Xi, Xingfeng; Si, Yang; Song, Hang; Wang, Jiangfeng; Cai, Kaiyong

doi:10.1016/j.actbio.2014.05.033

Cited by 37 publications

(20 citation statements)

References 50 publications

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“…On the other hand, formation of the mineralized matrix by osteoblasts grown on OPA/HAp coating decreased formation of the mineralized matrix, which could be related to lower activity of ALP activity and consequent lack of inorganic phosphate. Our results agreed with the data published by Yang et al, 51 who verified increased mineralization of osteoblasts grown on HAp coatings after 7 and 14 days of culture. Ozawa and Kasugai 52 detected enhanced formation of mineralized nodules when they used rat bone marrow cells differentiated into osteoblasts grown on a HAp implant material as compared to clean Ti.…”

Section: Formation Of Mineralized Matrixsupporting

confidence: 93%

Different compact hybrid Langmuir–Blodgett‐film coatings modify biomineralization and the ability of osteoblasts to grow

et al. 2018

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Calcium phosphates (CaPs) are biomaterials widely used in tissue regeneration with outstanding biological performance. Although the tremendous improvements achieved in CaP's materials research over the years, their interaction with physiological environments still need to be fully understood. The aim of this study is to explore a biomimetic Langmuir-Blodgett (LB) membrane to template the growth of hydroxyapatite (HAp) coatings on Ti surfaces and the ability of these coatings in inducing biomineralization by osteoblasts cultured in vitro. Changing the phospholipids (i.e., dihexadecyl phosphate (DHP) or octadecylphosphonic acid (OPA)), we also tuned the surface Ca concentration. This structural feature gave rise to different LB-hybrid surfaces where the concentration of Ca in the OPA/HAp was higher than the concentration of Ca in DHP/HAp coating. The higher Ca amount on OPA/HAp coatings, allied to the physical-chemical features, lead to different responses on osteoblasts, stimulating or inhibiting the natural biomineralization. The OPA/HAp coating caused a delay in the osteoblast proliferation as indicated by the decrease in the cell viability at the 7th culture day. Improved cell differentiation triggered by the DHP/HAp coating resulted in higher osteoblast biomineralization. The present data underscore that besides both coatings being composed by HAp, the final interfacial composition and physical-chemical properties influence differently the osteoblast behavior. Although the best osteoblast's viability was found to OPA/HAp, our dataset attested that DHP/HAp induced mineralization more effectively than that. This unexpected finding highlight the importance of deeply understanding the biomaterial interface and suggest a promising approach to the design of biofunctional LB-based coatings with tunable properties. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2524-2534, 2018.

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Section: Formation Of Mineralized Matrixsupporting

confidence: 93%

Different compact hybrid Langmuir–Blodgett‐film coatings modify biomineralization and the ability of osteoblasts to grow

et al. 2018

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“…Thus, biomaterials such as titanium (Ti) and titanium alloys (such as titanium–aluminum–vanadium alloy [Ti6Al4V]) are the most commonly used materials for dental implant owing to their high biocompatibility and mechanical properties . Topography influences cell behaviors, such as adhesion, proliferation, shape, migration, survival, and differentiation . In addition, a number of studies have shown that osteoblastic differentiation of osteoblast lineage cells increases when cells are cultured on Ti and Ti6Al4V substrates with micro‐scale roughness compared with those cultured on smooth surfaces .…”

Section: Introductionmentioning

confidence: 99%

Effects of the micro–nano surface topography of titanium alloy on the biological responses of osteoblast

Yin

Zhang

Cai

et al. 2016

J Biomedical Materials Res

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In clinical applications, osseointegration is essential for the long-term stability of dental implants. Inspired by the hierarchical structure of natural bone, we applied the electrochemical etching (EC) technique to form a micro-nano structure on a titanium alloy (Ti6Al4V) substrate, called EC surface. Sand blasting and acid etching (SLA) and machined (M) methods were employed to generate micro and smooth textures, respectively, as the control groups. The surface topographies of the three substrates were characterized using scanning electron microscopy (SEM). Then, human osteoblast-like cells (MG63) were cultured on substrates, and adhesion, proliferation, morphology, alkaline phosphatase activity (ALP), and gene expression levels of Runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN), and type I collagen (COLIA 1) were analyzed. MG63 cells cultured on the EC Ti alloy substrates displayed better cell adhesion, significant proliferation, and a higher production level of ALP, gene expressions of RUNX2, OCN, OPN and COLIA 1 (p < 0.01 or p < 0.05) compared with those of SLA and M substrates. These results indicate that the micro-nano structure fabricated by electrochemical etching method is beneficial for the biological functions of MG63 cells and may be a promising candidate in dental implants. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 757-769, 2017.

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“…Hopefully, the surface modification of titanium and its alloys can optimize the biological performance of Ti-based biomaterials, and at the same time retain their favorable mechanical properties. [4][5][6][7][8][9][10] When implanted into a living body, the surface physicochemical properties of the biomaterials determine the interactions between them and the surrounding biological environment. [10][11][12] Recent efforts in this field have highlighted the importance of modifying the surface topography and composition of materials to achieve good biological performance of implants.…”

Section: Introductionmentioning

confidence: 99%

Tuning the surface microstructure of titanate coatings on titanium implants for enhancing bioactivity of implants

Wang

Lai

Zheng

et al. 2015

IJN

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Biological performance of artificial implant materials is closely related to their surface characteristics, such as microtopography, and composition. Therefore, convenient fabrication of artificial implant materials with a cell-friendly surface structure and suitable composition was of great significance for current tissue engineering. In this work, titanate materials with a nanotubular structure were successfully fabricated through a simple chemical treatment. Immersion test in a simulated body fluid and in vitro cell culture were used to evaluate the biological performance of the treated samples. The results demonstrate that the titanate layer with a nanotubular structure on Ti substrates can promote the apatite-inducing ability remarkably and greatly enhance cellular responses. This highlights the potential of such titanate biomaterials with the special nanoscale structure and effective surface composition for biomedical applications such as bone implants.

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Surface engineering of titanium alloy substrates with multilayered biomimetic hierarchical films to regulate the growth behaviors of osteoblasts

Cited by 37 publications

References 50 publications

Different compact hybrid Langmuir–Blodgett‐film coatings modify biomineralization and the ability of osteoblasts to grow

Different compact hybrid Langmuir–Blodgett‐film coatings modify biomineralization and the ability of osteoblasts to grow

Effects of the micro–nano surface topography of titanium alloy on the biological responses of osteoblast

Tuning the surface microstructure of titanate coatings on titanium implants for enhancing bioactivity of implants

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