Surface engineering of cardiovascular stent with endothelial cell selectivity for in vivo re-endothelialisation

Yu, Wei; Ji, Ying; Xiao, Linlin; Lin, Quankui; Xu, Jian; Ren, Keke; Ji, Jian

doi:10.1016/j.biomaterials.2012.12.036

Cited by 172 publications

(144 citation statements)

References 41 publications

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“…[3][4][5][6]39 Cell-resistant surface can be generated via hydrophilic macromolecule immobilization or hydrogel-like surface coating. [40][41][42][43][44][45] In this study, we fabricated the zwitterionic PMPC brush onto the hydrophobic IOL surface via SI-RAFT polymerization. Such zwitterionic PMPC brush modification can effectively reduce the initial adhesion of residual LECs or bacteria, and is prospective in reducing the PCO incidence in vivo.…”

mentioning

confidence: 99%

Bottom-up fabrication of zwitterionic polymer brushes on intraocular lens for improved biocompatibility

Han¹,

Xu²,

Tang³

et al. 2016

IJN

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mentioning

confidence: 99%

Bottom-up fabrication of zwitterionic polymer brushes on intraocular lens for improved biocompatibility

Han¹,

Xu²,

Tang³

et al. 2016

IJN

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“…46,47 Those findings were concordant to previous reports, demonstrating that specificity of functionalization can be augmented by targeted bioactive sequence selection and combinatory application thereof. 15,22,25,39,48 Validation in a functional rodent aortic transplantation model under physiological flow conditions and full exposure to the native blood 7 failed to show a significant biological effect despite showing in vivo persistence of the dECM functionalization. Although a trend toward increased in vivo endothelialization of the functionalized dAoGs compared to the nonfunctionalized controls could be observed, it failed to reach statistical significance within the number of animals subjected to our model system and due to observed large biological variability of the early endothelialization process, which was also evident in the control group.…”

Section: Evaluation Of Biological Effects Of Functionalization Of Decmentioning

confidence: 99%

“…21 In particular, a biological blood-graft interface with high endothelial cell (EC) specificity, while suppressing activation of platelet attachment, immune cell recruitment, and smooth muscle cell overproliferation, is crucial in the process of in vivo endothelialization. 22 In this regard, cell-specific surface functionalization can be achieved using short synthetic peptides with defined bioactive sequences. 15,23 Interestingly, custom-made peptides, which are derived from ECM proteins but encompass only defined cell adhesive motifs, offer a series of advantages compared to the use of native proteins: they are chemically defined structures, show higher stability, and are devoid of immunogenicity.…”

Section: Introductionmentioning

confidence: 99%

Customized Interface Biofunctionalization of Decellularized Extracellular Matrix: Toward Enhanced Endothelialization

Aubin

Mas‐Moruno

Iijima

et al. 2016

Tissue Engineering Part C: Methods

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Interface biofunctionalization strategies try to enhance and control the interaction between implants and host organism. Decellularized extracellular matrix (dECM) is widely used as a platform for bioengineering of medical implants, having shown its suitability in a variety of preclinical as well as clinical models. In this study, specifically designed, custom-made synthetic peptides were used to functionalize dECM with different cell adhesive sequences (RGD, REDV, and YIGSR). Effects on in vitro endothelial cell adhesion and in vivo endothelialization were evaluated in standardized models using decellularized ovine pulmonary heart valve cusps (dPVCs) and decellularized aortic grafts (dAoGs), respectively. Contact angle measurements and fluorescent labeling of custommade peptides showed successful functionalization of dPVCs and dAoGs. The functionalization of dPVCs with a combination of bioactive sequences significantly increased in vitro human umbilical vein endothelial cell adhesion compared to nonfunctionalized controls. In a functional rodent aortic transplantation model, fluorescent-labeled peptides on dAoGs were persistent up to 10 days in vivo under exposure to systemic circulation. Although there was a trend toward enhanced in vivo endothelialization of functionalized grafts compared to nonfunctionalized controls, there was no statistical significance and a large biological variability in both groups. Despite failing to show a clear biological effect in the used in vivo model system, our initial findings do suggest that endothelialization onto dECM may be modulated by customized interface biofunctionalization using the presented method. Since bioactive sequences within the dECM-synthetic peptide platform are easily interchangeable and combinable, further control of host cell proliferation, function, and differentiation seems to be feasible, possibly paving the way to a new generation of multifunctional dECM scaffolds for regenerative medicine.

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“…1 Tissue-engineered vascular grafting is a promising solution that would provide patients with an alternative source of vascular replacements, thereby circumventing the shortage of autologous cells and avoiding diseased vessels. 2 To better assist cell engraftment and proliferation, surrogate scaffolds must be bioengineered to be as similar as possible to the native histo-architecture of the damaged vessel.…”

Section: Introductionmentioning

confidence: 99%

Composite vascular repair grafts via micro-imprinting and electrospinning

Liu

Chen

et al. 2015

AIP Advances

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Composite vascular grafts formed by micro-imprinting and electrospinning exhibited improved mechanical properties relative to those formed by electrospinning alone. The three-layered composite grafts mimic the three-layered structure of natural blood vessels. The middle layer is made by micro-imprinting poly-p-dioxanone (PPDO), while the inner and outer layers are electrospun mixtures of chitosan and polyvinyl alcohol. The graft morphology is characterized with scanning electron microscopy. For constant graft thicknesses, the PPDO increases the mechanical strength. Cells cultivated on the vascular grafts adhere and proliferate better because of the natural, biological chitosan in the inner and outer layers. Overall, the composite scaffolds could be good candidates for blood vessel repair.

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Surface engineering of cardiovascular stent with endothelial cell selectivity for in vivo re-endothelialisation

Cited by 172 publications

References 41 publications

Bottom-up fabrication of zwitterionic polymer brushes on intraocular lens for improved biocompatibility

Bottom-up fabrication of zwitterionic polymer brushes on intraocular lens for improved biocompatibility

Customized Interface Biofunctionalization of Decellularized Extracellular Matrix: Toward Enhanced Endothelialization

Composite vascular repair grafts via micro-imprinting and electrospinning

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