Surface Changes of Mineral Trioxide Aggregate after the Application of Bleaching Agents: Electron Microscopy and an Energy-dispersive X-Ray Microanalysis

Tsujimoto, Masaki; Ookubo, Atsushi; Wada, Yoko; Matsunaga, Tsunenori; Tsujimoto, Yasuhisa; Hayashi, Yoshihiko

doi:10.1016/j.joen.2010.11.013

Cited by 29 publications

(42 citation statements)

References 21 publications

(13 reference statements)

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“…This fact is indicative of a good cell substrate interaction signifying that both calcium-silicate based materials provide a significantly better substrate for cell adhesion when they set in the presence of citric acid 6,23 . A possible explanation is that the acidic conditions of the citric acid induced the release of Ca-ions, and subsequently the relative concentration of Si increased 30 . Furthermore, the acid-etching effect leads to microstructural changes that could affect the adhesion and proliferation of cells on calcium silicate-based materials 1,10,19,27 .…”

Section: Discussionmentioning

confidence: 99%

Interaction of dental pulp stem cells with Biodentine and MTA after exposure to different environments

et al. 2016

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Objective:The aim of the present study was to evaluate and compare the cytotoxic effects of Biodentine and MTA on dental pulp stem cells (DPSCs) and to assess cell viability and adherence after material exposure to an acidic environment.Material and Methods:DPSCs were cultured either alone or in contact with either: Biodentine; MTA set for 1 hour; or MTA set for 24 hours. After 4 and 7 days, cell viability was measured using the MTT assay. Biodentine and MTA were also prepared and packed into standardized bovine dentin disks and divided into three groups according to the storage media (n=6/group): freshly mixed materials without storage medium (Group A); materials stored in saline (Group B); materials stored in citric acid buffered at pH 5.4 (Group C). After 24 hours, DPSCs were introduced in the wells and cell adherence, viability, and cellular morphology were observed via confocal microscopy after three days of culture. Cell viability was analyzed using repeated-measures analysis of variance test with Tukey's post hoc tests (α=0.05).Results:Biodentine expressed significantly higher cell viability compared with all other groups after 4 days, with no differences after 7 days. Notably, cell viability was significantly greater in 24-hour set MTA compared with 1-hour set MTA and control groups after 7 days. Material exposure to an acidic environment showed an increase in cell adherence and viability in both groups.Conclusions:Biodentine induced a significantly accelerated cell proliferation compared with MTA. Setting of these materials in the presence of citric acid enhanced DPSC viability and adherence.

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Section: Discussionmentioning

confidence: 99%

Interaction of dental pulp stem cells with Biodentine and MTA after exposure to different environments

et al. 2016

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“…In clinical conditions, the sodium perborate and 30% hydrogen peroxide mixture are replenished at every 3-7 days, and the entire procedure might require multiple visits depending on the severity of discoloration (15). Authors reported that both materials caused deterioration proportional to the concentration of hydrogen peroxide on the surface structure of fully hardened MTA after bleaching (14). A sodium perborate and 30% hydrogen peroxide mixture was prepared and applied on the materials; then, the assembly was incubated at 37°C for 7 days.…”

Section: Discussionmentioning

confidence: 99%

“…A sodium perborate and 30% hydrogen peroxide mixture was prepared and applied on the materials; then, the assembly was incubated at 37°C for 7 days. These surface defects were reported to result from an interaction between MTA and hydrogen peroxide within the bleaching agent that leads to the formation of bubbles (14,19). Bleaching agents have been reported to exhibit negative effects on the mechanical properties of restorative materials and restorations (17,18).…”

Section: Discussionmentioning

confidence: 99%

“…One technique suggested by Belobrov and Parashos includes the removal of MTA, confirmation of the formation of a dentin bridge and the application of sodium perborate for 1 week (11). Bleaching agents have been reported to exhibit deteriorative affects on the surface of MTA by a previous scanning electron microscopy (SEM) study (14), although no study has evaluated the effect of sodium perborate and hydrogen peroxide bleaching on the compressive strength of calcium silicate-based cements. Moreover, due to its superior sealing ability, MTA has been suggested as a coronal barrier before bleaching procedures to prevent cervical root resorption (12,13).…”

Section: Introductionmentioning

confidence: 99%

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The effect of bleaching agents on the compressive strength of calcium silicate‐based materials

2018

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The present study aimed to evaluate the effect of sodium perborate on the compressive strength of calcium silicate-based materials. ProRoot wMTA, MTA Plus, NeoMTA Plus and Biodentine discs with 5 mm thickness and 4 mm diameter were prepared. Thirty discs from each material were used for compressive strength testing and divided into two groups: control and bleaching (n = 15). The sodium perborate was mixed with 30% hydrogen peroxide in a creamy consistency and placed on the surface of the specimens. Specimens were tested in an Instron machine, and compressive strength values were recorded and compared. The data were analysed using one-way ANOVA and post hoc Tukey tests. Compressive strength of all tested materials significantly decreased after bleaching (P < 0.05). SEM examination revealed deterioration on materials' surfaces after bleaching. Application of sodium perborate and hydrogen peroxide reduced the compressive strength of ProRoot wMTA, MTA Plus, NeoMTA Plus and Biodentine.

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“…Bleaching agents have also been shown to affect the elemental distribution of MTA [ 64 ]. A decrease in calcium and an increase in silicon were observed, and this tendency was especially pronounced when higher concentrations of hydrogen peroxide were used.…”

Section: Bleaching Agentsmentioning

confidence: 99%

Reactivity and Environmental Factors

Camilleri

Dummer

2014

Mineral Trioxide Aggregate in Dentistry

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Hydrated mineral trioxide aggregate (MTA) is composed of un-hydrated cement particles, which acts as a core around which a reaction rim of hydration products is formed ( Fig. 2.5 ). When mixed with water, the tricalcium and dicalcium silicate react forming calcium silicate hydrate (honeycomb appearance) and calcium hydroxide (hexagonal plates) (Fig. 2.6 ), which is leached in solution. The presence of calcium hydroxide has been verifi ed by X-ray diffraction analysis as it produces a typical peak for portlandite at 18° 2 θ (Fig. 2.9 ), whilst the leaching of calcium has been measured by inductively coupled plasma. The particular microstructure of MTA and the presence of calcium hydroxide in solution make the material susceptible to environmental factors. The use of MTA brings it into contact with other materials, tissues and fl uids, and a number of changes occur within or on the surface of the material in various environmental conditions. Effect of Environmental Factors on the Set MaterialMTA has a variety of applications mostly in Endodontics. These various uses have been discussed [ 60 ] and are outlined in Chap. 6 . Dental Materials Glass IonomerCovering MTA with glass ionomer cement when it is used as a pulp capping agent results in physical changes to the materials at their interface ( Fig. 5.1a ). A sizable gap has been observed at the interface attributed to the movement of water out of MTA into the glass ionomer. This movement of water out of MTA can inhibit its hydration and increases its porosity [ 9 ]. There is no evidence that covering MTA with a glass ionomer has an effect on the glass ionomer cement itself with regard to setting time and setting reaction [ 5 , 36 ]. However, migration of strontium from glass ionomer to MTA has been observed [ 9 ], although its effects are unknown. Base MaterialsZinc oxide eugenol-based cements are used as temporary fi lling materials and come into contact with MTA when it is used for pulp capping. Zinc is a retarder of cement hydration [ 47 ], and zinc salts form calcium hydrozincate (Ca(Zn(OH) 3 H 2 O) 2 ), an insoluble hydroxide in alkaline solution that creates a coating on MTA particles. In addition, zinc

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Surface Changes of Mineral Trioxide Aggregate after the Application of Bleaching Agents: Electron Microscopy and an Energy-dispersive X-Ray Microanalysis

Cited by 29 publications

References 21 publications

Interaction of dental pulp stem cells with Biodentine and MTA after exposure to different environments

Interaction of dental pulp stem cells with Biodentine and MTA after exposure to different environments

The effect of bleaching agents on the compressive strength of calcium silicate‐based materials

Reactivity and Environmental Factors

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