Surface and protein analyses of normal human cell attachment on PIII-modified chitosan membranes

Saranwong, N.; Inthanon, Kewalin; Wongkham, Weerah; Wanichapichart, Pikul; Suwannakachorn, D.; Yu, L.D.

doi:10.1016/j.nimb.2011.01.107

Cited by 13 publications

(5 citation statements)

References 18 publications

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“…The formation of nanoscale texture by argon plasma treatment has facilitated the increase in hydrophilicity, cell attachment, growth and proliferation. Saranwong et al (2012) modified the chitosan with argon and nitrogen PIII for human skin fibroblast F1 544 cell attachment. The authors found that the argon plasma treatment showed enhanced cell attachment while nitrogen plasma had an inhibiting effect.…”

Section: Bioactive Molecules To Promote Cell Adhesion and Proliferationmentioning

confidence: 99%

Plasma treatments of dressings for wound healing: a review

Eswaramoorthy

McKenzie

2017

Biophys Rev

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This review covers the use of plasma technology relevant to the preparation of dressings for wound healing. The current state of knowledge of plasma treatments that have potential to provide enhanced functional surfaces for rapid and effective healing is summarized. Dressings that are specialized to the needs of individual cases of chronic wounds such as diabetic ulcers are a special focus. A summary of the biology of wound healing and a discussion of the various types of plasmas that are suitable for the customizing of wound dressings are given. Plasma treatment allows the surface energy and air permeability of the dressing to be controlled, to ensure optimum interaction with the wound. Plasmas also provide control over the surface chemistry and in cases where the plasma creates energetic ion bombardment, activation with long-lived radicals that can bind therapeutic molecules covalently to the surface of the dressing. Therapeutic innovations enabled by plasma treatment include the attachment of microRNA or antimicrobial peptides. Bioactive molecules that promote subsequent cell adhesion and proliferation can also be bound, leading to the recruitment of cells to the dressing that may be stem cells or patient-derived cells. The presence of a communicating cell population expressing factors promotes healing.

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Section: Bioactive Molecules To Promote Cell Adhesion and Proliferationmentioning

confidence: 99%

Plasma treatments of dressings for wound healing: a review

Eswaramoorthy

McKenzie

2017

Biophys Rev

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“…Currently, silk and chitosan 34,35 are the only two natural polymers that have been PIII‐treated, with silk being the only natural polymer on which covalent immobilization of a biomolecule has been demonstrated 29,30 . In particular, when rDV was immobilized on PIII‐treated silk, rDV‐PIII‐silk supported excellent blood interactions when rDV was present, but PIII‐silk was thrombogenic in the absence of rDV 30 .…”

Section: Introductionmentioning

confidence: 99%

Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium

Lau

Zhang

et al. 2023

J Biomedical Materials Res

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Strategies to promote rapid formation of functional endothelium are required to maintain blood fluidity and regulate smooth muscle cell proliferation in synthetic vascular conduits. In this work, we explored the biofunctionalization of silk biomaterials with recombinantly expressed domain V of human perlecan (rDV) to promote endothelial cell interactions and the formation of functional endothelium. Perlecan is essential in vascular development and homeostasis and rDV has been shown to uniquely support endothelial cell, while inhibiting smooth muscle cell and platelet interactions, both key contributors of vascular graft failure. rDV was covalently immobilized on silk using plasma immersion ion implantation (PIII), a simple one‐step surface treatment process which enables strong immobilization in the absence of chemical cross‐linkers. rDV immobilization on surface‐modified silk was assessed for amount, orientation, and bio‐functionality in terms of endothelial cell interactions and functional endothelial layer formation. rDV immobilized on PIII‐treated silk (rDV‐PIII‐silk) supported rapid endothelial cell adhesion, spreading, and proliferation to form functional endothelium, as evidenced by the expression of vinculin and VE‐cadherin markers. Taken together, the results provide evidence for the potential of rDV‐PIII‐silk as a biomimetic vascular graft material.

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“…The material and electrodes are immersed in gas plasma so that the surface modification is conformal around the material, ensuring that even complex three-dimensional structures can be treated . PIII treatment has been extensively explored for modification and functionalization of synthetic polymers − and has only recently been demonstrated on silk materials, to our knowledge the only natural polymer other than chitosan , to have undergone PIII treatment to date …”

Section: Introduction To Dry Surface Treatment Techniquesmentioning

confidence: 99%

Dry Surface Treatments of Silk Biomaterials and Their Utility in Biomedical Applications

Lau

Akhavan

Lord

et al. 2020

ACS Biomater. Sci. Eng.

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Silk-based materials are widely used in biomaterial and tissue engineering applications due to their cytocompatibility and tunable mechanical and biodegradation properties. Aqueous-based processing techniques have enabled the fabrication of silk into a broad range of material formats, making it a highly versatile material platform across multiple industries. Utilizing the full potential of silk in biomedical applications frequently requires modification of silk's surface properties. Dry surface modification techniques, including irradiation and plasma treatment, offer an alternative to the conventional wet chemistry strategies to modify the physical and chemical properties of silk materials without compromising their bulk properties. While dry surface modification techniques are more prevalent in textiles and sterilization applications, the range of modifications available and resultant changes to silk materials all point to the utility of dry surface modification for the development of new, functional silk biomaterials. Dry surface treatment affects the surface chemistry, secondary structure, molecular weight, topography, surface energy, and mechanical properties of silk materials. This Review describes and critically evaluates the effect of physical dry surface modification techniques, including irradiation and plasma processes, on silk materials and discusses their utility in biomedical applications, including recent examples of modulation of cell/protein interactions on silk biomaterials, in vivo performance of implanted biomaterials, and applications in material biofunctionalization and lithographic surface patterning approaches.

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Surface and protein analyses of normal human cell attachment on PIII-modified chitosan membranes

Cited by 13 publications

References 18 publications

Plasma treatments of dressings for wound healing: a review

Plasma treatments of dressings for wound healing: a review

Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium

Dry Surface Treatments of Silk Biomaterials and Their Utility in Biomedical Applications

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