Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium

Lau, Kieran; Fu, Lu; Zhang, Anyu; Akhavan, Behnam; Whitelock, John M.; Bilek, M.M.M.; Lord, Megan S.; Rnjak‐Kovacina, Jelena

doi:10.1002/jbm.a.37525

Cited by 5 publications

(1 citation statement)

References 59 publications

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“… 89 , 91 , 102 , 103 Perlecan domain V promotes laying down of an endothelium and has useful traits that promote incorporation of vascular grafts and improves the performance of coated implants in tissue repair. 18 , 104 , 105 The 80 kDa C‐terminal fragment of perlecan in degenerate IVDs may promote ingrowth of blood vessels into the degenerate IVD 106 and in chondroid metaplasia in degenerate IVDs. 68 …”

Section: Introductionmentioning

confidence: 99%

Diverse and multifunctional roles for perlecan (HSPG2) in repair of the intervertebral disc

Melrose,

Guilak

2024

JOR Spine

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Perlecan is a widely distributed, modular, and multifunctional heparan sulfate proteoglycan, which facilitates cellular communication with the extracellular environment to promote tissue development, tissue homeostasis, and optimization of biomechanical tissue functions. Perlecan‐mediated osmotic mechanotransduction serves to regulate the metabolic activity of cells in tissues subjected to tension, compression, or shear. Perlecan interacts with a vast array of extracellular matrix (ECM) proteins through which it stabilizes tissues and regulates the proliferation or differentiation of resident cell populations. Here we examine the roles of the HS‐proteoglycan perlecan in the normal and destabilized intervertebral disc. The intervertebral disc cell has evolved to survive in a hostile weight bearing, acidic, low oxygen tension, and low nutrition environment, and perlecan provides cytoprotection, shields disc cells from excessive compressive forces, and sequesters a range of growth factors in the disc cell environment where they aid in cellular survival, proliferation, and differentiation. The cells in mechanically destabilized connective tissues attempt to re‐establish optimal tissue composition and tissue functional properties by changing the properties of their ECM, in the process of chondroid metaplasia. We explore the possibility that perlecan assists in these cell‐mediated tissue remodeling responses by regulating disc cell anabolism. Perlecan's mechano‐osmotic transductive property may be of potential therapeutic application.

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Section: Introductionmentioning

confidence: 99%

Diverse and multifunctional roles for perlecan (HSPG2) in repair of the intervertebral disc

Melrose,

Guilak

2024

JOR Spine

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Tuning Recombinant Perlecan Domain V to Regulate Angiogenic Growth Factors and Enhance Endothelialization of Electrospun Silk Vascular Grafts

Jiang,

Yang,

Tan

et al. 2024

Adv Healthcare Materials

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Synthetic vascular grafts are used to bypass significant arterial blockage when native blood vessels are unsuitable, yet their propensity to fail due to poor blood compatibility and progressive graft stenosis remain an intractable challenge. Perlecan is the major heparan sulphate (HS) proteoglycan in the blood vessel wall with an inherent ability to regulate vascular cell activities associated with these major graft failure modes. Here we tune the ability of the engineered form of perlecan domain V (rDV) to bind angiogenic growth factors and support endothelial cell proliferation via the composition of its glycosaminoglycan chain. We show that the HS on rDV supports angiogenic growth factor signalling, including fibroblast growth factor 2 and vascular endothelial growth factor (VEGF)165, while both HS and chondroitin sulphate on rDV are involved in VEGF189 signalling. We also show that physisorption of rDV on emerging electrospun silk fibroin vascular grafts promote endothelialisation and patency in a murine arterial interposition model, compared to the silk grafts alone. Together, this study demonstrates the potential of rDV as a tuneable, angiogenic biomaterial coating which both potentiates growth factors and regulates endothelial cells.This article is protected by copyright. All rights reserved

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Electrospun polyhedral oligomeric silsequioxane-poly(carbonate-urea) urethane for fabrication of hemocompatible small-diameter vascular grafts with angiogenesis capacity

Zakeri,

Salehi,

Rahbarghazi

et al. 2024

International Journal of Biological Macromolecules

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Recombinant perlecan domain V covalently immobilized on silk biomaterials via plasma immersion ion implantation supports the formation of functional endothelium

Cited by 5 publications

References 59 publications

Diverse and multifunctional roles for perlecan (HSPG2) in repair of the intervertebral disc

Diverse and multifunctional roles for perlecan (HSPG2) in repair of the intervertebral disc

Tuning Recombinant Perlecan Domain V to Regulate Angiogenic Growth Factors and Enhance Endothelialization of Electrospun Silk Vascular Grafts

Electrospun polyhedral oligomeric silsequioxane-poly(carbonate-urea) urethane for fabrication of hemocompatible small-diameter vascular grafts with angiogenesis capacity

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