Dry Surface Treatments of Silk Biomaterials and Their Utility in Biomedical Applications

Lau, Kieran; Akhavan, Behnam; Lord, Megan S.; Bilek, Marcela M.M.; Rnjak‐Kovacina, Jelena

doi:10.1021/acsbiomaterials.0c00888

Cited by 25 publications

(14 citation statements)

References 155 publications

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“…Physical modifications of SF scaffolds include ultraviolet (UV) treatment, gas treatment and plasma treatment (Lau et al, 2020). UV irradiation of SF scaffolds can increase wettability and improve cell adhesion without obvious weight loss, crystallinity change and strength decrease (Li et al, 2012;Khosravi et al, 2018).…”

Section: Surface Modification Of Sf Hybrid Scaffolds 431 Physical Mod...mentioning

confidence: 99%

Silk fibroin scaffolds: A promising candidate for bone regeneration

Lin²,

Zhao³

et al. 2022

Front. Bioeng. Biotechnol.

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It remains a big challenge in clinical practice to repair large-sized bone defects and many factors limit the application of autografts and allografts, The application of exogenous scaffolds is an alternate strategy for bone regeneration, among which the silk fibroin (SF) scaffold is a promising candidate. Due to the advantages of excellent biocompatibility, satisfying mechanical property, controllable biodegradability and structural adjustability, SF scaffolds exhibit great potential in bone regeneration with the help of well-designed structures, bioactive components and functional surface modification. This review will summarize the cell and tissue interaction with SF scaffolds, techniques to fabricate SF-based scaffolds and modifications of SF scaffolds to enhance osteogenesis, which will provide a deep and comprehensive insight into SF scaffolds and inspire the design and fabrication of novel SF scaffolds for superior osteogenic performance. However, there still needs more comprehensive efforts to promote better clinical translation of SF scaffolds, including more experiments in big animal models and clinical trials. Furthermore, deeper investigations are also in demand to reveal the degradation and clearing mechanisms of SF scaffolds and evaluate the influence of degradation products.

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Section: Surface Modification Of Sf Hybrid Scaffolds 431 Physical Mod...mentioning

confidence: 99%

Silk fibroin scaffolds: A promising candidate for bone regeneration

Lin²,

Zhao³

et al. 2022

Front. Bioeng. Biotechnol.

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“…For both strategies, the spectra showed distinct signals of carbon, nitrogen, and oxygen with almost the same atomic concentrations of around 65, 30, and 5%, respectively (Figure b). No silicon signal was detected, indicating that the silicon wafer underneath the layer of nanoparticles was covered with layers of particles greater than the sensitivity depth of XPS, which is around 10 nm. − The origin of the oxygen content on the surface of the nanoparticles is suggested to be either from residual oxygen in the plasma chamber or from the exposure to atmosphere post plasma polymerization. Due to the relatively low base pressure (below 5 × 10 –5 Torr), oxidation reactions that take place postproduction are the more likely contributors to the presence of oxygen on the PPNs.…”

Section: Resultsmentioning

confidence: 99%

Surface-Active Plasma-Polymerized Nanoparticles for Multifunctional Diagnostic, Targeting, and Therapeutic Probes

Haidar

Baldry

Fraser

et al. 2022

ACS Appl. Nano Mater.

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Surface-functionalized polymeric nanoparticles have advanced the field of nanomedicine as promising constructs for targeted delivery of molecular cargo as well as diagnostics and therapeutics. Conventionally, the functionalization of polymeric nanoparticles incorporates tedious wet chemical methods that require complex, multistep protocols. Surface-active plasma-polymerized nanoparticles (PPNs) produced by a dry, low-pressure plasma process can be easily functionalized with multiple ligands in a simple step. However, plasma polymerization remains limited by the challenge of efficient collection of PPNs from low-pressure plasma reactors. Here, we demonstrate a simple method to overcome this limitation by delaying the inflow of the polymer-forming precursor gas, acetylene, into a nitrogen and argon plasma discharge. We provide evidence that this cutting-edge development in the plasma polymerization method drastically enhances the collection yield of nanoparticles by 2.5-fold, compared to the simultaneous inflow of the gases. COMSOL Multiphysics simulations support our experimental data and provide insights into the role of pressure gradients in regulating the forces controlling the collection of the particles. Surface characterization data revealed that changing the sequence of the precursor gas inflow had no significant effect on the physicochemical properties of the nanoparticles, as critically important for theranostic applications. A model, green fluorescent protein, was successfully conjugated to the surface of the PPNs via a reagent-free, one-step incubation process that immobilized the biomolecule while retaining its biological activity. Cytotoxicity of the particles was assessed by a lactate dehydrogenase (LDH) assay at concentrations of up to 5 × 105 nanoparticles per cell. Despite their high concentrations, the nanoparticles were remarkably well tolerated by the cells, demonstrating their superb potential for in vivo cellular uptake. This study advances previous research on plasma-polymerized nanoparticles, introducing a low-waste synthesis method that achieves higher yields. This sustainable technology has important implications for the production of multifunctional nanoparticles for drug delivery, tumor targeting, and medical imaging.

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“…The XPS survey spectra presented in Figure 1c show the presence of carbon and oxygen elements in the shellac coatings. XPS has a sampling depth of around 10 nm, [30][31][32][33] and thus no signals from the substrate were detected. The C, O, and Si atomic concentrations for the SH10 sample are 78.46%, 19.56%, and 1.98%, while those measured for the SH50 sample are 80.97%, 17.03% and 2.00%, respectively.…”

Section: Fabrication and Characterization Of Shellac Coatingsmentioning

confidence: 99%

Shellac: A Bioactive Coating for Surface Engineering of Cardiovascular Devices

Yang

Yong

Yue

et al. 2022

Adv Materials Inter

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materials due to the surface-induced clotting and thrombus formation that can cause device failure. [1,2] The proliferation of smooth muscle cells (SMCs) on the surfaces of foreign materials may lead to restenosis upon implantation of cardiovascular devices such as stents. [3] The intimal hyperplasia and thrombosis are the main reasons that lead to low retrievable rate of inferior vena cava filters (IVCs). [4] It is a major challenge to create a safe bloodcontacting surface balancing cytocompatibility and antithrombogenic effects. [2,[5][6][7][8][9][10] In addition, the bacterial infection of cardiovascular devices such as catheters and artificial blood vessels must be avoided, as it may also cause implant failures, while being life-threatening in extreme cases. [11] Shellac (also known as lac) is a natural resin that can potentially be used as a bioactive material to modify the surfaces of blood-contacting devices. Shellac is secreted by shellac insects that parasitize on branches of certain legumes by sucking the sap. [12] It is composed of polyester and single ester with polyhydroxypolybasic acids such as aleuritic acid, jalaric acid, and laccijalaric acid with the chemical structure depicted in Figure 1a. [13] The hydrophobic (aleuritic acid) and hydrophilic (cyclic terpene acid) components of shellac are linked with each other through ester bonds, providing it with an amphiphilic nature. [13] Shellac

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Dry Surface Treatments of Silk Biomaterials and Their Utility in Biomedical Applications

Cited by 25 publications

References 155 publications

Silk fibroin scaffolds: A promising candidate for bone regeneration

Silk fibroin scaffolds: A promising candidate for bone regeneration

Surface-Active Plasma-Polymerized Nanoparticles for Multifunctional Diagnostic, Targeting, and Therapeutic Probes

Shellac: A Bioactive Coating for Surface Engineering of Cardiovascular Devices

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