Silk fibroin scaffolds: A promising candidate for bone regeneration

Wu, Hao; Lin, Kaili; Zhao, Chong; Wang, Xudong

doi:10.3389/fbioe.2022.1054379

Cited by 13 publications

(9 citation statements)

References 162 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SF was chosen owing to its outstanding characteristics, including structural adaptability, controllable biodegradability, and minimal immunologic responses when used in living organisms [22]. The β-sheet formation of SF confers superior mechanical properties compared with other natural polymers, including collagen and chitosan, with an ultimate tensile strength in the range of several hundred megapascals [23]. However, the use of high-molecular-weight SF requires harsh solubilization conditions, limiting its practicality as a biocompatible material.…”

Section: Resultsmentioning

confidence: 99%

Silver nitrate-assisted photo-crosslinking for enhancing the mechanical properties of an alginate/silk fibroin-based 3D scaffold

Moon,

Choi,

Cha

et al. 2024

Biofabrication

View full text Add to dashboard Cite

Scaffolds play a pivotal role in tissue engineering and serve as vital biological substitutes providing structural support for cell adhesion and subsequent tissue development. An ideal scaffold must possess mechanical properties suitable for tissue function and exhibit biodegradability. Although synthetic polymer scaffolds offer high rigidity and elasticity owing to their reactive side groups, which facilitate tailored mechanical and rheological properties, they may lack biological cues and cause persistent side effects during degradation.To address these challenges, natural polymers have garnered attention owing to their inherent bioactivity and biocompatibility. However, natural polymers such as silk fibroin (SF) and tyramine-modified alginate–tyramine (AT) have limitations, including uncontrolled mechanical properties and weak structural integrity. In this study, we developed a blend of SF and AT as a printable biomaterial for extrusion-based 3D printing. The use of photocrosslinkable SF/AT inks facilitated the fabrication of complex scaffolds with high printability, thereby enhancing their structural stability. The incorporation of silver nitrate facilitated the tunability of mechanical and rheological behaviors. SF/AT scaffolds with varying stiffness in the physiologically relevant range for soft tissues (51–246 kPa) exhibited excellent biocompatibility, indicating their promising potential for diverse applications in tissue engineering.

show abstract

Section: Resultsmentioning

confidence: 99%

Silver nitrate-assisted photo-crosslinking for enhancing the mechanical properties of an alginate/silk fibroin-based 3D scaffold

Moon,

Choi,

Cha

et al. 2024

Biofabrication

View full text Add to dashboard Cite

show abstract

“…[91,93] In addition, cross-linking agents like methanol or ethanol stabilize the scaffold structure. [94,95] Post-printing treatments, such as freeze-drying or annealing, may enhance the stability of SF scaffolds. [96,97] Optimizing these fabrication parameters is crucial for successfully creating SF-based fibrous scaffolds with the requisite mechanical properties and biocompatibility for tissue engineering applications.…”

Section: D Bioprintingmentioning

confidence: 99%

Fabrication of Silk Fibroin‐Derived Fibrous Scaffold for Biomedical Frontiers

Rahman,

Dip,

Nur

et al. 2024

Macro Materials & Eng

View full text Add to dashboard Cite

Silk fibroin (SF), a natural protein derived from silkworms, has emerged as a promising biomaterial due to its biocompatibility, biodegradability, degradation rate, and tunable mechanical properties. This review delves into the intrinsic attributes of SF that make it an attractive candidate for scaffold development in tissue engineering and regenerative medicine. The distinctiveness of this comprehensive review resides in its detailed exploration of recent advancements in the fabrication techniques of SF‐based fibrous scaffolds, namely electrospinning, freeze‐drying, and 3D printing. An in‐depth analysis of these fabrication techniques is conducted to illustrate their versatility in customizing essential scaffold characteristics, such as porosity, fiber diameter, and mechanical strength. The article meticulously discusses process parameters, advantages, and challenges of each fabrication technique, highlighting the innovative advancements made in the respective field. Furthermore, the review goes beyond fabrication techniques to provide an overview of the latest biomedical applications and research endeavors utilizing SF‐derived scaffolds. From nerve regeneration and wound healing to drug delivery, bone regeneration, and vascular tissue engineering, the diverse applications underscore the versatility of SF in adopting various biomedical challenges. Finally, the article emphasizes the need for standardized characterization techniques, scalable manufacturing processes, and long‐term in vivo studies.

show abstract

“…[83] It has good mechanical properties, supports cell adhesion and can be manipulated to produce different scaffold morphologies, including porous meshes, hydrogels and microparticles. [84] As such, it has been fairly used for bone tissue engineering, [85,86,87] while its use in 3D in vitro OS models has only recently been reported. [88,89,90] Alginate: Alginate is a polysaccharide obtained from brown seaweed and is an interesting biomaterial for 3D OS models due to its similarities to the glycosaminoglycans (GAGs) found in the bone TME.…”

Section: Natural Biomaterialsmentioning

confidence: 99%

From Spheroids to Bioprinting: A Literature Review on Biomanufacturing strategies of 3D In vitro Osteosarcoma Models

Domingues,

Silva,

Sanjuan-Alberte

2024

Preprint

View full text Add to dashboard Cite

Osteosarcoma (OS) is a rare primary malignant bone cancer affecting mainly young individuals. Treatment typically consists of chemotherapy and surgical tumor resection, which has undergone few improvements since the 1970s. This therapeutic approach encounters several limitations attributed to the tumor’s inherent chemoresistance, marked heterogeneity and metastatic potential. Therefore, the development of in vitroplatforms that closely mimic the OS pathophysiology is crucial to understand tumor progression and discover effective anticancer therapeutics. Contrary to 2D monolayer cultures and animal models, 3Din vitro platforms show promise in replicating the 3D tumor macrostructure, cell-cell and cell-extracellular matrix interactions. This review provides an overview of the biomanufacturing strategies employed in developing 3D in vitro OS models, highlighting their role in replicating different aspects of OS and improving OS anticancer research and drug screening. A variety of 3D in vitro models are explored, including both scaffold-free and scaffold-based models, encompassing cell spheroids, hydrogels, and innovative approaches like electrospun nanofibers, microfluidic devices and bioprinted constructs. By examining the distinctive features of each model type, this review offers insights into their potential transformative impact on the landscape of OS research and therapeutic innovation, addressing the challenges and future directions of 3D in vitro OS modeling.

show abstract

Silk fibroin scaffolds: A promising candidate for bone regeneration

Cited by 13 publications

References 162 publications

Silver nitrate-assisted photo-crosslinking for enhancing the mechanical properties of an alginate/silk fibroin-based 3D scaffold

Silver nitrate-assisted photo-crosslinking for enhancing the mechanical properties of an alginate/silk fibroin-based 3D scaffold

Fabrication of Silk Fibroin‐Derived Fibrous Scaffold for Biomedical Frontiers

From Spheroids to Bioprinting: A Literature Review on Biomanufacturing strategies of 3D In vitro Osteosarcoma Models

Contact Info

Product

Resources

About