Suppressive Activity of Histamine H1 Receptor Antagonists, Desloratadine and Levocetirizine, on the Production of Periostin from Nasal Epithelial Cells In vitro

Abstract

Background: Periostin, a 90-kDa endogenous extracellular matrix protein, is well known to be involved in the development and persistence of allergic rhinitis. Although histamine H1 receptor antagonists are recommended as first choice of agents in the treatment of allergic rhinitis, the influence of the agents on periostin production is not well understood. The present study was undertaken to examine the influence of histamine H1 receptor antagonists on periostin production from nasal epithelial cells after IL-4 stimulation in vitro. Methods: Human nasal epithelial cells (HNEpC) at a concentration of 1 × 10 5 cells/ml were stimulated with 10.0 ng/ml IL-4 in combination with either desloratadine (DLT), loratadine (LT), levocetirizine (LCT) or cetirizine (CT). After 48 h, culture supernatants were collected and assayed for periostin levels by ELISA. The influence of LCT on transcription factor, STAT6, activation and periostin mRNA expression were also examined by ELISA and real-time RT-PCR, respectively. Results: Treatment of cells with DLT, LT, LCT and CT suppressed the ability of HNEpC to produce periostin in response to IL-4 stimulation in dose-dependent manner. The minimum concentration that caused significant suppression is 0.01 µM for DLT, 0.05 µM for LT, 0.05 µM for LCT and 0.1 µM for CT. Treatment of HNEpC with LCT at more than 0.05 µM also suppressed STAT6 activation and periostin mRNA expression induced by IL-4 stimulation. Conclusion: The present results strongly suggest that histamine H1 receptor antagonists favorably modify the clinical conditions of allergic rhinitis through the suppression of periostin production from nasal epithelial cells after IL-4 stimulation.

Pharmacological Benefits of Triphala: A Perspective for Allergic Rhinitis

“…The figure was created by BioRender.com. addition, periostin, a novel marker of respiratory inflammation, and thioredoxin, an antioxidant enzyme, were found to be moderated by quercetin, resulting in an improvement in AR clinical condition (Irie et al, 2016;Edo et al, 2018). The effects of quercetin were also revealed in the clinical setting in which the combination of herbs [quercetin, Perilla frutescens (L.) Britton, and vitamin D3] known as "Lertal ® " was effective in relieving the overall symptoms of AR and reducing the use of anti-allergic drugs without additional adverse effects (Thornhill and Kelly, 2000).…”

“…Alleviates nasal inflammation in a mouse model of OVA-induced AR (Fan et al, 2019) Alleviation of nasal symptoms caused by inflammatory cytokines and autacoids Inhibits of pro-inflammatory cytokines and histamine release (Kim et al, 2006) Reduction of oxidative stress in AR and may suppress exacerbation of AR-related symptoms Suppresses AR-related mediators both in vitro and in vivo (Ebihara et al, 2018;Edo et al, 2018) Suppresses pro-inflammatory cytokines and inflammatory inducers (Mlcek et al, 2016) Reduces markers of respiratory inflammation (Irie et al, 2016;Edo et al, 2018) Inhibits IL-33-mediated ILC2 activation and subsequent Th2 cytokine release via downregulation of the MyD88/NF-κB signaling pathway in ovalbumin-induced asthma in mice (Wang et al, 2018) Antioxidant (Roidoung et al, 2016) Vitamin C…”