Suppression of miR-93-5p inhibits high-risk HPV-positive cervical cancer progression via targeting of BTG3

Li, Jie; Chu, Zhaoping; Han, Hua; Zhang, Yuan; Tian, Fei; Zhang, Junqin; Huang, Xianghua

doi:10.1007/s13577-018-00225-1

Cited by 22 publications

(20 citation statements)

References 38 publications

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“…The results indicated that in SW480 cells, miR‐93‐5p regulated PD‐L1 specifically and negatively, which was previously found in pancreatic cancer (Cioffi et al, 2017); moreover, we also noted that transfection of miR‐93‐5p mimics decreased the level of PD‐L1, and this effect was abolished with the administration of inhibitors; in PD‐L1‐negative patients, miR‐93‐5p expression was significantly elevated, suggesting the key role of miR‐93‐5p targeting PD‐L1 . miR‐93‐5p, a member of the miR‐106b‐25 gene cluster located on chromosome 7q22, is not only an oncogene in many tumors (Li et al, 2018, 2019) but is also a tumor suppressor in certain tumors (Shyamasundar et al, 2016; Xiang et al, 2017). The results of this study suggested that miR‐93‐5p expression was downregulated in the tumor tissues and had a significant association with the major clinicopathological features and survival of patients.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐93‐5p expression in tumor tissue and its tumor suppressor function via targeting programmed death ligand‐1 in colorectal cancer

Chen

Wang

Lin

et al. 2020

Cell Biology International

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This work aimed to investigate miR-93-5p expression in tumor tissue and its in vitro effects in colorectal cancer (CRC) by targeting programmed death ligand-1 (PD-L1). MiR-93-5p and PD-L1 expression was detected in CRC and adjacent normal tissues by quantitative real-time polymerase chain reaction and immunohistochemistry. The correlation between miR-93-5p and PD-L1 was validated by a dual-luciferase reporter assay. HCT116 and SW480 cells were divided into blank, miR-NC, miR-93-5p mimics, miR-93-5p inhibitor, PD-L1 small interfering RNA (siRNA) and miR-93-5p inhibitor + PD-L1 siRNA groups, and wound-healing and transwell assays were performed to detect cell migration and invasion, respectively. Protein expression was measured by western blotting. The secretion of cytokines was detected in the CRC cell/T coculture models. MiR-93-5p was downregulated in CRC tissues with upregulated PD-L1. In PD-L1-negative patients, miR-93-5p expression was increased compared with that in PD-L1-positive patients. MiR-93-5p and PD-L1 expression levels were associated with the tumor differentiation, lymphatic metastasis, TNM, Duke's stage, and prognosis of CRC. PD-L1 siRNA weakened the migration and invasion abilities via decreased expression of matrix metalloproteinase-1 (MMP-1), -2, and -9, and these effects were abolished by the miR-93-5p inhibitor. Additionally, anti-PD-L1 upregulated the expressions of interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α), and interferon γ (IFN-γ) in the coculture of T cells with CRC cells, but downregulated the expressions of IL-1β, IL-10, and TGF-β. However, these changes were partially reversed by miR-93-5p inhibition. miR-93-5p is expected to be a novel target for CRC treatment since it decreases the migration and invasion, as well as the immune evasion, of CRC cells via targeting PD-L1. Abbreviations: CRC, colorectal cancer; ELISA, enzyme-linked immunosorbent assay; HE, hematoxylin and eosin; ITIM, immunoreceptor tyrosinebased inhibitory motif; miRNA, microRNA; PD-L1, programmed death ligand-1 Role of miR-93-5p in CRC via targeting PD-L1Y.-L. Chen et al.

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Section: Discussionmentioning

confidence: 99%

MicroRNA‐93‐5p expression in tumor tissue and its tumor suppressor function via targeting programmed death ligand‐1 in colorectal cancer

Chen

Wang

Lin

et al. 2020

Cell Biology International

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“…12 These studies suggest that the abnormal expression of miRNAs may affect the malignant progression of CC. It has been reported that miR-145-5p is down-regulated in a variety of human cancers, such as breast cancer 13 and bladder cancer 14 , and plays an inhibitory role in tumor growth. In addition, miR-145 has been confirmed to have the potential serving as a candidate biomarker for human diagnosis.…”

Section: Introductionmentioning

confidence: 99%

<p>miR-145-5p Regulates the Proliferation, Migration and Invasion in Cervical Carcinoma by Targeting KLF5</p>

Cao¹,

Pan²,

Tang³

et al. 2020

OTT

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Objective: Cervical carcinoma (CC) is a serious threat to women's health and few effective therapeutic methods have been discovered. The purpose of this study is to explore the underlying mechanism of miR-145-5p in CC. Methods: Bioinformatics methods were employed to analyze the gene expression data of CC from TCGA database. qRT-PCR was used to detect the expression of miR-145-5p and KLF5 in CC cells, and Western blot was employed for the examination of KLF5 protein level. The targeted relationship between miR-145-5p and KLF5 was verified by a dualluciferase reporter assay. Moreover, CCK-8, wound healing assay and transwell invasion assay were used to analyze the effects of miR-145-5p overexpression or KLF5 silencing on the proliferation, migration and invasion of CC cells. Results: miR-145-5p was shown to be down-regulated in CC tissues and cells, while KLF5 was up-regulated. miR-145-5p could bind to the complementary sequence within the wild type KLF5 3ʹUTR rather than the mutant one. In addition, miR-145-5p could effectively down-regulate KLF5, in turn inhibiting the proliferation, migration and invasion of CC cells. Conclusion: miR-145-5p regulates the proliferation, migration and invasion of CC cells by targeting KLF5.

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“…3 The symptoms of CC normally occur at the advanced stage, leading to the vitiation of the optimum treatment time and high mortality, particularly in developing countries. 4,5 In addition, smoking, age, race and secondary malignancies caused by invasiveness are critical risk factors of poor prognosis. 6 Therefore, the discovery of novel biomarkers is imperative for the therapy of CC.…”

Section: Introductionmentioning

confidence: 99%

Retracted Article: Long non-coding RNA NEAT1 accelerates cell progression in cervical cancer by regulating the miR-889-3p/E2F7 axis through the activation of the PI3K/AKT pathway

2019

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Suppression of miR-93-5p inhibits high-risk HPV-positive cervical cancer progression via targeting of BTG3

Cited by 22 publications

References 38 publications

MicroRNA‐93‐5p expression in tumor tissue and its tumor suppressor function via targeting programmed death ligand‐1 in colorectal cancer

MicroRNA‐93‐5p expression in tumor tissue and its tumor suppressor function via targeting programmed death ligand‐1 in colorectal cancer

<p>miR-145-5p Regulates the Proliferation, Migration and Invasion in Cervical Carcinoma by Targeting KLF5</p>

Retracted Article: Long non-coding RNA NEAT1 accelerates cell progression in cervical cancer by regulating the miR-889-3p/E2F7 axis through the activation of the PI3K/AKT pathway

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