Supported Hybrid Bilayer Membranes as Rugged Cell Membrane Mimics

Plant, Anne L.

doi:10.1021/la981662t

Cited by 324 publications

(326 citation statements)

References 36 publications

(85 reference statements)

Supporting

Mentioning

316

Contrasting

Unclassified

Order By: Relevance

“…[5][6][7] Only noncovalent interaction forces hold the lipids in these membranes together; thus, these bilayer systems are intrinsically unstable and collapse or desorb when removed from an aqueous environment and exposed to the ambient atmosphere or ultra-high-vacuum conditions. [8][9][10][11][12] This is a major limitation for applications of PSLBs in sensors and other types of devices, as well as implantable biomaterial surfaces. Additionally, many surface-sensitive analytical techniques such as X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) that operate under vacuum and provide detailed chemical characterization information cannot be used to analyze PSLBs.…”

Section: Introductionmentioning

confidence: 99%

Ultra-High Vacuum Surface Analysis Study of Rhodopsin Incorporation into Supported Lipid Bilayers

et al. 2008

View full text Add to dashboard Cite

Planar supported lipid bilayers that are stable under ambient atmospheric and ultra-high-vacuum conditions were prepared by cross-linking polymerization of bis-sorbylphosphatidylcholine (bisSorbPC). X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) were employed to investigate bilayers that were cross-linked using either redox-initiated radical polymerization or ultraviolet photopolymerization. The redox method yields a more structurally intact bilayer; however, the UV method is more compatible with incorporation of transmembrane proteins. UV polymerization was therefore used to prepare cross-linked bilayers with incorporated bovine rhodopsin, a light-activated, G-protein-coupled receptor (GPCR). A previous study (Subramaniam, V.; Alves, I. D.; Salgado, G. F. J.; Lau, P. W.; Wysocki, R. J.; Salamon, Z.; Tollin, G.; Hruby, V. J.; Brown, M. F.; Saavedra, S. S. J. Am. Chem. Soc. 2005, 127, 5320-5321) showed that rhodopsin retains photoactivity after incorporation into UV-polymerized bis-SorbPC, but did not address how the protein is associated with the bilayer. In this study, we show that rhodopsin is retained in supported bilayers of poly(bis-SorbPC) under ultra-high-vacuum conditions, on the basis of the increase in the XPS nitrogen concentration and the presence of characteristic amino acid peaks in the ToF-SIMS data. Angle-resolved XPS data show that the protein is inserted into the bilayer, rather than adsorbed on the bilayer surface. This is the first study to demonstrate the use of ultra-high-vacuum techniques for structural studies of supported proteolipid bilayers.

show abstract

Section: Introductionmentioning

confidence: 99%

Ultra-High Vacuum Surface Analysis Study of Rhodopsin Incorporation into Supported Lipid Bilayers

et al. 2008

View full text Add to dashboard Cite

show abstract

“…4B. It comprises an electrolyte resistance Rs, a lipid bilayer capacitance Cdl, which consisted of the bare GC electrode (CGC) and PC membrane (Cm), 19,20 …”

Section: Characterization Of Sblm and Au Nanoparticles Hybrid Sblm Onmentioning

confidence: 99%

Detection of Cytochrome c at Biocompatible Nanostructured Au-lipid Bilayer-modified Electrode

Liu

Wei

2008

ANAL. SCI.

View full text Add to dashboard Cite

“…A variety of non-fouling materials have been synthesized during the past decades and some of them have been successfully applied worldwide, such as poly(ethylene glycol) (PEG) and poly(ethylene oxide) (PEO) [9][10][11][12]. Supported phospholipid membrane has also shown good validity in preventing protein adsorption [13][14][15]. With its self-assembly property, flexibility and two-dimensional fluidity, phospholipid membranes have promising applications in the study of membrane proteins or ion channels [16].…”

Section: Introductionmentioning

confidence: 99%

Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry

Zhang

Chen

Jin

2011

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

a b s t r a c tWe describe highly sensitive, label-free, real time detection of the biomarker CA19-9 using microarray biosensors based on total internal reflection imaging ellipsometry (TIRIE), in which anti-CA19-9 is immobilized onto the PEGylated phospholipid membrane. Strong resistance against non-specific adsorption of PEGylated lipid membrane allows direct serum assay without the blocking agent. We find that PEG density has an influence on antibody assembly and only a proper amount of PEG is appropriate for antigen binding. Two different methods for antibody assembly are compared and finally protein A is preferred due to its higher protein binding efficiency. The estimated detection limit of CA19-9 is 18.2 U/ml, which is lower than the cut-off value for normal level (37 U/ml). Regression analysis between the results of microarray biosensor and electrochemiluminescence immunoassay (ECLIA) suggests a high correlation (R = 0.989). The capability for real-time monitoring of tumor marker with high sensitivity and selectivity in clinically relevant samples opens up substantial possibilities for early cancer diagnosis and treatment.

show abstract

Supported Hybrid Bilayer Membranes as Rugged Cell Membrane Mimics

Cited by 324 publications

References 36 publications

Ultra-High Vacuum Surface Analysis Study of Rhodopsin Incorporation into Supported Lipid Bilayers

Ultra-High Vacuum Surface Analysis Study of Rhodopsin Incorporation into Supported Lipid Bilayers

Detection of Cytochrome c at Biocompatible Nanostructured Au-lipid Bilayer-modified Electrode

Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry

Contact Info

Product

Resources

About