Detection of Cytochrome c at Biocompatible Nanostructured Au-lipid Bilayer-modified Electrode

Liu, Yaxiong; Wei, Wanzhi

doi:10.2116/analsci.24.1431

Cited by 17 publications

(12 citation statements)

References 24 publications

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“…Thus, the biosensor is rather selective and sensitive to cyt c. The LOD calculated as signal to noise ratio S/N = 3 (10 nM) is approximately 5 times better as compared with that of a recently reported biosensor [16], which however did not utilize a specific receptor. The LOD of the capacitive sensor reported in [14] is comparable with that reported in the present work but that sensor was not selective to cyt c and it demonstrates a much better LOD (1 nM) for polylysine.…”

Section: Resultsmentioning

confidence: 67%

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Electrochemical Impedance Spectroscopy for Assessing the Recognition of Cytochrome c by Immobilized Calixarenes

et al. 2011

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We developed a biosensor based on supported lipid films (sBLM) with incorporated calixarenes for detection of cytochrome c (cyt c) using electrochemical impedance spectroscopy (EIS). Calix[6]arene (CALIX) was incorporated into soybean phosphatidylcholine (SBPC) liposomes in different molar ratios (SBPC:CALIX = 10 : 1, 30 : 1 and 100 : 1). The lipid layer containing calixarenes has been formed on the surface of a gold electrode previously covered with an octadecanethiol monolayer. Both the sBLM capacitance and the electron transfer resistance showed noticeable modification at a cyt c concentration as low as 10 nM. However, a much higher concentration of free lysine (> 30 mM) was necessary to induce a similar effect. Nonspecific interaction of cyt c with sBLM without CALIX was negligible.

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Section: Resultsmentioning

confidence: 67%

“…A further improvement of the LOD has been reported by Lu and Wei [16] who used a similar system of SBPC and LA on GC, but the lipid layer has been modified by gold nanoparticles. This resulted in a substantial improvement of the LOD which brought to 50 nM.…”

Section: Introductionmentioning

confidence: 88%

Electrochemical Impedance Spectroscopy for Assessing the Recognition of Cytochrome c by Immobilized Calixarenes

et al. 2011

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“…Later on, Liu and Wei described the detection of cyt c at biocompatible gold nanoparticle supported bilayer lipid membrane (sBLM) modified with anionic sites (Liu and Wei, 2008). They have deposited Au nanoparticles electrochemically through the sBLM modified with lauric acid to build the anionic sites modified nanoscale electrode array to detect cyt c. The detection limit of this electrode was 50 nM for cyt c (S/N = 3) and the linear range of cyt c concentration spans between 1.0X 10 -7 and 3.2X10 -6 M. Similarly, Zhao et al, have also constructed a selfassembled gold nanoparticles multilayer architecture on a gold electrode based on the recognition between complementary oligo-DNA strands (Zhao et al, 2008).…”

Section: Dna Based Cyt C Biosensorsmentioning

confidence: 99%

Recent advances in cytochrome c biosensing technologies

Manickam

Kaushik

Karunakaran³

et al. 2017

Biosensors and Bioelectronics

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This review is an attempt, for the first time, to describe advancements in sensing technology for cytochrome c (cyt c) detection, at point-of-care (POC) application. Cyt c, a heme containing metalloprotein is located in the intermembrane space of mitochondria and released into bloodstream during pathological conditions. The release of cyt c from mitochondria is a key initiative step in the activation of cell death pathways. Circulating cyt c levels represents a novel in-vivo marker of mitochondrial injury after resuscitation from heart failure and chemotherapy. Thus, cyt c detection is not only serving as an apoptosis biomarker, but also is of great importance to understand certain diseases at cellular level. Various existing techniques such as enzyme-linked immunosorbent assays (ELISA), Western blot, high performance liquid chromatography (HPLC), spectrophotometry and flow cytometry have been used to estimate cyt c. However, the implementation of these techniques at POC application is limited due to longer analysis time, expensive instruments and expertise needed for operation. To overcome these challenges, significant efforts are being made to develop electrochemical biosensing technologies for fast, accurate, selective, and sensitive detection of cyt c. Presented review describes the cutting edge technologies available in the laboratories to detect cyt c. The recent advancements in designing and development of electrochemical cyt c biosensors for the quantification of cyt c are also discussed. This review also highlights the POC cyt c biosensors developed recently, that would prove of interest to biologist and therapist to get real time informatics needed to evaluate death process, diseases progression, therapeutics and processes related with mitochondrial injury.

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“…Recently, amperometric sensors for the direct determination of cyt c with good detection limits were reported (Zhao et al, 2008;Liu and Wei, 2008). It plays a key role in the biological respiratory chain, whose function is to transfer electrons between cytochrome c reductase (CcR) (complex III) and cytochrome c oxidase (CcO) (complex IV).…”

Section: Cytochrome C Oxidase and Reductase-based Cytochrome C Biosenmentioning

confidence: 99%

Enzymatic Biosensors

Karunakaran¹,

Thangamuthu²,

Sethy

2015

Biosensors and Bioelectronics

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Detection of Cytochrome c at Biocompatible Nanostructured Au-lipid Bilayer-modified Electrode

Cited by 17 publications

References 24 publications

Electrochemical Impedance Spectroscopy for Assessing the Recognition of Cytochrome c by Immobilized Calixarenes

Electrochemical Impedance Spectroscopy for Assessing the Recognition of Cytochrome c by Immobilized Calixarenes

Recent advances in cytochrome c biosensing technologies

Enzymatic Biosensors

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