Supplemented tissue culture medium 199 is a better medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human tubal fluid

The effects of different types of media on in vitro maturation outcomes of human germinal vesicle oocytes retrieved in intracytoplasmic sperm injection cycles

“…One study in patients with polycystic ovary syndrome (PCOS) found TCM-199 culture medium to be superior to HTF-supplemented medium for oocyte maturation, fertilization, and subsequent embryo development; they reported findings similar to those of the present study regarding the maturation rate (56.9% vs. 55.5%), fertilization rate (39.4% vs. 53%), and embryo quality (41.7% vs. 28.5%). They concluded that HTF medium is not an appropriate medium for the maturation of oocytes obtained from PCOS patients in non-stimulated cycles [ 8 ]. As well, de Araujo et al [ 8 ] reported that TCM 199 was better than HTF medium in the IVM of oocytes obtained from PCOS patients in terms of the maturation rate (82% vs. 56.9%), fertilization rate (70% vs. 39.4%), and embryo quality (81.3% vs. 41.7%).…”

“…They concluded that HTF medium is not an appropriate medium for the maturation of oocytes obtained from PCOS patients in non-stimulated cycles [ 8 ]. As well, de Araujo et al [ 8 ] reported that TCM 199 was better than HTF medium in the IVM of oocytes obtained from PCOS patients in terms of the maturation rate (82% vs. 56.9%), fertilization rate (70% vs. 39.4%), and embryo quality (81.3% vs. 41.7%). In contrast, Kim et al [ 10 ] demonstrated that TCM-199 culture medium was unsuitable in comparison with Sage and blastocyst media.…”

“…For IVM success, the choice of a suitable culture medium plays an important role, since low oocyte maturation and embryo development rates following IVM are considered major complications [ 5 6 7 ]. Various media for IVM have been proposed in different clinical or research studies, including human tubal fluid (HTF) [ 8 ], culture medium 199 [ 9 10 ], and blastocyst medium [ 10 ]. Additionally, there are two commercially available IVM media, MediCult (Origio, Jyllinge, Denmark) and Sage IVM media (Cooper Surgical, Trumbull, CT, USA), which are often used with reasonable fertilization, implantation, and pregnancy rates [ 11 ].…”

Factors Influencing the In Vitro Maturation (IVM) of Human Oocyte

“…Common media mainly include the tissue culture medium 199 (TCM-199), human tubal fluid (HTF), Ham’s-F10 medium, glucose-free medium (P1), Chang’s medium, and blastocyst medium [ 37 , 38 , 39 ]. Based on existing literature, TCM-199 is the most commonly used non-tailor-made medium for human oocyte IVM [ 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ].…”

“…Based on existing literature, TCM-199 is the most commonly used non-tailor-made medium for human oocyte IVM [ 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ]. It has also been reported that the performance of TCM-199 in human oocyte IVM is superior to HTF with respect to maturation rate, fertilization rate, and embryo quality [ 39 ]. However, another study revealed that the glucose-free P1 medium is superior to TCM-199 (which contains a high glucose concentration) for IVM of human granulosa cell-free oocytes [ 9 ].…”