The effects of different types of media on in vitro maturation outcomes of human germinal vesicle oocytes retrieved in intracytoplasmic sperm injection cycles

Abstract

ObjectiveOptimizing in vitro maturation (IVM) media to achieve better outcomes has been a matter of interest in recent years. The aim of this prospective clinical trial was to investigate the effects of different media on the IVM outcomes of immature oocytes at the germinal vesicle (GV) stage.MethodsA total of 400 immature oocytes at the GV stage with normal morphology were retrieved from 320 infertile women aged 31±4.63 years during stimulated intracytoplasmic sperm injection (ICSI) cycles. They were divided into groups of homemade IVM medium (I, n=100), cleavage medium (II, n=100), blastocyst medium (III, n=100), and Sage IVM medium (IV, n=100) and cultured for 24 to 48 hours at 37℃. ICSI was performed, and the rates of fertilization and embryo formation were compared across the four groups.ResultsIn the 400 retrieved GV oocytes, the total maturation rates showed significant differences in groups I to IV (55%, 53%, 78%, and 68%, respectively, p<0.001). However, there were no significant differences in the fertilization, embryo formation, or arrest rates of metaphase II oocytes across these groups. In all groups, GV maturation was mostly completed after 24 hours, with fewer oocytes requiring 48 hours to mature (p<0.01). Moreover, the rate of high-quality embryos was higher in group IV than in the other groups (p=0.01).ConclusionThe quality of the IVM medium was found to affect clinical IVM outcomes. Additionally, blastocyst medium may be a good choice in IVM/ICSI cycles as an alternative IVM medium.

Growth Hormone Promotes in vitro Maturation of Human Oocytes

“…Obviously, the 200 ng/ml was the optimal concentration. In this study, the maturation rate of IVM in the control group was 35%, which was close to the literature report of 38% (32). The maturation rate of 200 ng/ml GH group reached 70% which was double of the control group, and this indicated that GH could remarkably promote nucleus maturation of human oocytes.…”

Immature Oocyte for Fertility Preservation

“…The complex culture media as the basic IVM media have been used in research or in clinical purposes of human immature oocyte culture for IVM (60). Recently, commercialized IVM media such as SAGE (Coopersurgical) IVM medium and Medi-Cult IVM medium have been used in several IVF centers as they have the advantage of being certified as IVF quality controlled (61–63). However, no IVM medium is superior to other media.…”

“…However, no IVM medium is superior to other media. You could choose an IVF media as a convenient basic media such as blastocyst media (61, 63). Serum albumin and gonadotropins are typically supplemented to the IVM medium.…”

Comprehensive molecular features of polycystic ovary syndrome revealed by transcriptome analysis of oocytes and cumulus cells

“…In vitro maturation of germinal vesicle oocytes was achieved using SAGE IVM media kit (ART-1600). Briefly, germinal vesicle oocytes surrounded by CCs, retrieved during IVF cycles, were washed twice with SAGE washing medium and then cultured in SAGE IVM medium with 75 IU HMG (human menopausal gonadotropin) for approximately 24 h under paraffin oil at 37°C in high humidified atmosphere of 6% CO2 in air 85 . Oocyte maturation was assessed by the presence of the first polar body using the inverted microscope.…”