Superswelling Microneedle Arrays for Dermal Interstitial Fluid (Prote)Omics

Laszlo, Elise; Crescenzo, Grégory De; Nieto-Argüello, Alfonso; Banquy, Xavier

doi:10.1002/adfm.202106061

Cited by 31 publications

(32 citation statements)

References 106 publications

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“…Recently, Zheng et al reported the collection of 13 μL of dermal ISF from the pig skin ex vivo in 20 min using MNs formulated with methacrylated hyaluronic acid and maltose. 41 Laszlo et al have designed superswelling acrylate-based MN arrays to rapidly collect 15 μL of dermal ISF in the excised pig skin in less than 1 h. 29 Chang et al have reported that 2.3 μL of ISF in the mouse in 10 min could be extracted by the cross-linked MeHA-MN patch. 42 He et al developed a point-of-care testing (POCT)platform based on hydrogel microneedle patches, which can extract 7.5 mg of skin ISF from the mimetic skin model.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Microneedles (MNs) that consist of an array of dozens of needles with a height of 50–1500 μm, a tip diameter of 1–100 μm, and a bottom width of 25–500 μm, can effectively avoid the stimulation of dermal nerve endings for pain-free delivery. − Recently, MNs-based transdermal delivery has been designed for various biomedical applications such as the treatment of neuropathic pain, eye diseases, hair regrowth, , obesity, , diabetes mellitus, − cancer, − influenza vaccination research, and contraception . However, little effort has been devoted to the MNs-related ISF diagnosis, which was mainly limited by the unsatisfied sampling capacity, sampling kinetics, as well as the low abundance of biomarkers in the ISF. − A MNs-based platform for the longitudinal sampling of cells and ISF was developed with the limitation on detecting targets with high natural abundance in the skin ISF . Sulaiman et al have first reported an MNs detection system for sampling and detecting specific nucleic acid biomarkers from ISF, and the reports were accomplished on human skin ex vivo models for the low swelling rate of MNs .…”

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confidence: 99%

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A Sample and Detection Microneedle Patch for Psoriasis MicroRNA Biomarker Analysis in Interstitial Fluid

Qiao

et al. 2022

Anal. Chem.

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Skin interstitial fluid (ISF) containing a great variety of molecular biomarkers derived from cells and subcutaneous blood capillaries has recently emerged as a clinically potential component for early diagnosis of a wide range of diseases; however, the minimally invasive sampling and detection of cell-free biomarkers in ISF is still a key challenge. Herein, we developed microneedles (MNs) that consist of gelatin methacryloyl (GelMA) and graphene oxide (GO) for the enrichment and sensitive detection of multiple microRNA (miRNA) biomarkers from skin ISF. The GO-GelMA MNs exhibited robust mechanical properties, fast sampling kinetics, and large swelling capacity, which enabled collecting ISF volume high to 21.34 μL in 30 min, facilitating effective miRNA analysis. It preliminarily realized the sensitive detection of three types of psoriasis-related miRNAs biomarkers either on the patch itself or in solution after release from the hydrogel by combining catalytic hairpin assembly signal amplification reaction. The automated and minimally invasive ISF miRNA detection technology of GO-GelMA MNs has great potential to monitor cell-free clinically informative biomarkers for personalized diagnosis and prognosis.

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Section: ■ Results and Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A Sample and Detection Microneedle Patch for Psoriasis MicroRNA Biomarker Analysis in Interstitial Fluid

Qiao

et al. 2022

Anal. Chem.

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“…[27] In addition, ISF was non-coagulable and had the potential to achieve continuous monitoring. [28] Omic analysis could identify biomarkers, Elise et al [29] designed the MNs made of superabsorbent acrylate-based hydrogel and used them for ISF sampling to explore the proteome. After proteomic analysis, the researchers detected 176 clinically relevant biomarkers in the collected samples, indicating the potential of ISF as an important source of biomarkers for disease monitoring and diagnosis.…”

Section: The Composition Of Isfmentioning

confidence: 99%

Microneedle‐Based Technology: Toward Minimally Invasive Disease Diagnostics

Zhang

et al. 2022

Adv Materials Technologies

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biomarkers by extracting ISF and can also be used as electrodes to detect blood glucose, tumor biomarkers and pH value in body fluids in real time.MNs are composed of a series of microsized needles and are widely used in transdermal drug delivery systems (TDDS) due to the advantages of high efficiency, safety and convenience. In recent years, MNs have been frequently used to deliver drugs, genes, proteins, RNAs and vaccines, and have achieved amazing therapeutic effects. [5] Additionally, MNs are also widely studied for blood sampling, signal monitoring and biosensor. [6] The new coronavirus COVID-19 is raging around the world. Researchers had developed a fingertip-sized MNs to deliver the vaccine. The results showed that the vaccine could produce SARS-CoV-2 specific antibodies in mice that could neutralize the virus. [7] Zhou et al. [8] loaded the anticancer drug curcumin into the MNs, and verified the antitumor effects of MNs in the melanoma model. In addition, MNs had also been used in clinical practice, and researchers had evaluated the efficacy and safety of MNs administration based on the effects of clinical trials. The results indicated that MNs showed similar or more efficient results compared with conventional treatment methods. [9] Interstitial fluid (ISF) is an alternative source to detect traditional biomarkers such as blood and urine. [10] Recently, a new method has emerged, that is, using MNs to extract ISF and analyze biomarkers in ISF. [11] The materials for preparing the MNs are usually metals or polymers. The tips length of MNs is about 50-900 µm, which can penetrate the skin stratum corneum to extract ISF. [12] However, ISF has received limited attentions from researchers due to the difficulty of extracting ISF. It is very necessary to develop a minimally invasive technique to sample ISF for clinical monitoring and diagnosis.ISF can be collected by techniques such as micro dialysis or open flow perfusion, these techniques not only require local anesthesia and professional operations but also take a certain amount of time, and there is a potential risk of infection. [13] It is a promising technique to extract ISF by inserting MNs into skin to form micropores. The average depth of the dermis is generally 500-2000 µm. [14] The tips of MNs are generally short, and they can penetrate the superficial layer of the skin but cannot reach the nerves and blood vessels in the dermis, which can painlessly extract ISF. [15] The biggest challenge of extracting ISF for analysis is insufficient sampling, which greatly limits The traditional way of disease diagnosis is to detect biomarkers in serum, blood, urine, and saliva, but it often faces wound infection and pain, and usually requires professional medical personnel to operate, which is unacceptable for most people. As a result, minimally invasive detection of biomarkers has attracted wide attention and is expected to become a new method for patient monitoring and disease diagnosis. Interstitial fluid (ISF) acts as a medium between cells and the circulatory ...

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“…The developed GelMA MN patch exhibits an efficient extraction of ISF. Laszlo et al (2021) proposed two hydrogel-based MN arrays for dermal ISF proteomics sampling. The developed MN patches could restore their initial shape even after multiple mandatory washing steps to erase un-crosslinked polymers.…”

Section: Microneedles For Transdermal Samplingmentioning

confidence: 99%

Microneedle-Based Device for Biological Analysis

Zada

Yang

et al. 2022

Front. Bioeng. Biotechnol.

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The collection and analysis of biological samples are an effective means of disease diagnosis and treatment. Blood sampling is a traditional approach in biological analysis. However, the blood sampling approach inevitably relies on invasive techniques and is usually performed by a professional. The microneedle (MN)-based devices have gained increasing attention due to their noninvasive manner compared to the traditional blood-based analysis method. In the present review, we introduce the materials for fabrication of MNs. We categorize MN-based devices based on four classes: MNs for transdermal sampling, biomarker capture, detecting or monitoring analytes, and bio-signal recording. Their design strategies and corresponding application are highlighted and discussed in detail. Finally, future perspectives of MN-based devices are discussed.

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Superswelling Microneedle Arrays for Dermal Interstitial Fluid (Prote)Omics

Cited by 31 publications

References 106 publications

A Sample and Detection Microneedle Patch for Psoriasis MicroRNA Biomarker Analysis in Interstitial Fluid

A Sample and Detection Microneedle Patch for Psoriasis MicroRNA Biomarker Analysis in Interstitial Fluid

Microneedle‐Based Technology: Toward Minimally Invasive Disease Diagnostics

Microneedle-Based Device for Biological Analysis

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